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布氏白僵菌侵染杨干象幼虫体壁的扫描电镜及透射电镜观察

曹庆杰 迟德富 宇佳 冉亚丽

曹庆杰, 迟德富, 宇佳, 冉亚丽. 布氏白僵菌侵染杨干象幼虫体壁的扫描电镜及透射电镜观察[J]. 北京林业大学学报, 2015, 37(5): 96-101. doi: 10.13332/j.1000-1522.20140483
引用本文: 曹庆杰, 迟德富, 宇佳, 冉亚丽. 布氏白僵菌侵染杨干象幼虫体壁的扫描电镜及透射电镜观察[J]. 北京林业大学学报, 2015, 37(5): 96-101. doi: 10.13332/j.1000-1522.20140483
CAO Qing-jie, CHI De-fu, YU Jia, RAN Ya-li. SEM and TEM observations of Beauveria brongniartii(Sacc.)Petch infecting body wall of Cryptorhynchus lapathi L. (Coleoptera: Curculionidae) larvae.[J]. Journal of Beijing Forestry University, 2015, 37(5): 96-101. doi: 10.13332/j.1000-1522.20140483
Citation: CAO Qing-jie, CHI De-fu, YU Jia, RAN Ya-li. SEM and TEM observations of Beauveria brongniartii(Sacc.)Petch infecting body wall of Cryptorhynchus lapathi L. (Coleoptera: Curculionidae) larvae.[J]. Journal of Beijing Forestry University, 2015, 37(5): 96-101. doi: 10.13332/j.1000-1522.20140483

布氏白僵菌侵染杨干象幼虫体壁的扫描电镜及透射电镜观察

doi: 10.13332/j.1000-1522.20140483
基金项目: 

“十二五”国家科技支撑计划项目(2012BAD19B07-04)。

详细信息
    作者简介:

    曹庆杰,博士生。主要研究方向: 森林害虫防治。Email: 767635662@qq.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 迟德富,教授,博士生导师。主要研究方向:森林害虫防治。Email: chidefu@126.com 地址: 同上。

    曹庆杰,博士生。主要研究方向: 森林害虫防治。Email: 767635662@qq.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 迟德富,教授,博士生导师。主要研究方向:森林害虫防治。Email: chidefu@126.com 地址: 同上。

SEM and TEM observations of Beauveria brongniartii(Sacc.)Petch infecting body wall of Cryptorhynchus lapathi L. (Coleoptera: Curculionidae) larvae.

  • 摘要: 为了解布氏白僵菌对杨干象幼虫的侵染过程,利用扫描电镜与透射电镜研究了布氏白僵菌对杨干象幼虫体壁的侵染过程及超微结构变化。扫描电镜结果显示:接种12 h后,分生孢子成功附着于杨干象幼虫体壁不同区域,如气孔、感器周围与节间膜部位;接种24 h后,最早观察到布氏白僵菌以芽管或菌丝的形式穿透杨干象幼虫体壁,无附着孢产生;接种90、96 h后,由虫体内穿出的菌丝在虫体表面开始大量繁殖。透射电镜结果显示:菌丝在穿透过程中伴随着机械压力与组织溶解现象。接种第24 h时,菌丝穿入表皮;到第36 h时,大量菌丝侵入到皮细胞层中;到第48 h时,菌丝侵入皮细胞层后使皮细胞层发生变形,菌丝周围出现电子密度很低的光晕;到第60 h,表皮层与皮细胞层分离,菌丝周围的细胞器溶解,细胞核遭到破坏,菌丝周围出现絮状溶解物,这种现象在国内外均未见报道,证实了菌丝侵入杨干象幼虫体内过程中有酶的参与。到第72 h时,细胞内排列松弛变成空泡;第84 h时,菌丝段以无隔、1个隔或2个隔的方式在血腔内大量繁殖;到第96 h,菌丝从表皮中穿出。

     

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  • 收稿日期:  2014-12-29

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