菊花花色嵌合花瓣的离体培养及植株再生
Culture in vitro and plant regeneration from petals of flower color chimera in chrysanthemum
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摘要: 以菊花粉妆'粉色和其发生嵌合花色的花瓣为试材,研究了6-BA 和NAA 不同浓度配比对花瓣离体再生的 影响,建立了花瓣外植体的再生体系,观察分析了花色嵌合花瓣再生植株的花色变异情况。结果表明:菊花品种 粉妆'花瓣再生的最适配方为MS +6-BA2.0 mg/ L + NAA1.0 mg/ L,再生芽分化率达93.3%,单个外植体平均再生 不定芽数为10.0 个,采用1/2MS 培养基,生根率和移栽成活率达100.0%。花瓣再生植株的花色发生广泛变异,粉 色花瓣再生植株的花色呈现从白色到黄色多色变异,且粉色占的比例最高,但没有花色嵌合体出现;而嵌合花色的 花瓣再生植株发生变异的花色更为丰富,从白色到橙红色均有分布,且与亲本相同或相似的花色占的比例最高,部 分植株还可以保持花色嵌合性状,但嵌合的花色仅为白色与粉色的嵌合,嵌合花色形成率为4%;因此,菊花品种 粉妆'经过组织培养可以部分保持嵌合花色。Abstract: A research work was conducted to evaluate the effects of 6-BA in combination with NAA on in vitro propagation of chrysanthemum Fenzhuang'petal explants to establish the regeneration system of chrysanthemum petals, and to investigate the flower color variation in plants regenerated from petals of flower color chimera. MS medium supplemented with 2.0 mg/ L6-BA +1.0 mg/ LNAA was found to be optimum combination concentration for shoot bud regeneration. The differentiation rate of regenerating buds was 93.3%, and average number of 10 regenerating buds per explant was observed at above combination concentration. The rooting rate and transplanting survival rate was 100.0% recorded on 1/2 MS medium. Extensive color variation in flower color was observed in petal explants regenerated plants. The regenerated plant (grown from pink petals) flower color ranged from white to yellow with the highest rate of pink color flowering plants, but there was no flower color chimera. More flower color variation from white to salmon, with highest rate of parental plants flower color was observed in plants regenerated from petals of flower color chimera. It was found that some plants can keep flower color chimerism, but only the chimeric colors of white and pink with 4% of chimeric color formation rate were achieved. Thus, tissue culture method can partly keep chimeric color for chrysanthemum Fenzhuang'.