Abstract: In this study, the shoots of Tamarix chinensis were treated with eight kinds of hormone combinations (two level NAA and four level 6-BA) and five types medium (MS, 1/2 MS, NT, B5 and WPM) and the growth and contents of secondary metabolite products were investigated in tamarix plantlet. The results showed that different hormone combinations and different medium had significant effects on the growth and accumulation of secondary products of T. chinensis seedlings. MS supplemented with 6-BA (1.0 mg/L) and NAA (0.05 mg/L) resulted in the optimal growth of T. chinensis. Under different treatments, the highest secondary product was phenol (136.84 - 353.58 mg/g), followed by triterpenoids (17.65 - 63.13 mg/g) and total flavonoids content (11.10 - 18.92 mg/g). When hormones 6-BA (1.0 mg/L) and NAA (0.05 mg/L) were added into different type medium, total phenolic yield of the 1/2 MS was the highest, while the total triterpenoids production in MS medium was the highest (up to 9.13 mg). Total flavonoids yield of 1/2 MS and MS medium was 3.25 and 2.50 mg, respectively. This study lays a foundation for the large-scale production of T. chinensis medicinal ingredients through the process of biological fermentation.