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欧美杨脱水素PdDHN2b的克隆与表达分析

郭鹏 邢鑫 张万筠 姜健

郭鹏, 邢鑫, 张万筠, 姜健. 欧美杨脱水素PdDHN2b的克隆与表达分析[J]. 北京林业大学学报, 2015, 37(1): 22-36. doi: 10.13332/j.cnki.jbfu.2015.01.017
引用本文: 郭鹏, 邢鑫, 张万筠, 姜健. 欧美杨脱水素PdDHN2b的克隆与表达分析[J]. 北京林业大学学报, 2015, 37(1): 22-36. doi: 10.13332/j.cnki.jbfu.2015.01.017
GUO Peng, XING Xin, ZHANG Wan-jun, JIANG Jian. Cloning and characterization of PdDHN2b gene from Populus deltoides×Populus nigra.[J]. Journal of Beijing Forestry University, 2015, 37(1): 22-36. doi: 10.13332/j.cnki.jbfu.2015.01.017
Citation: GUO Peng, XING Xin, ZHANG Wan-jun, JIANG Jian. Cloning and characterization of PdDHN2b gene from Populus deltoides×Populus nigra.[J]. Journal of Beijing Forestry University, 2015, 37(1): 22-36. doi: 10.13332/j.cnki.jbfu.2015.01.017

欧美杨脱水素PdDHN2b的克隆与表达分析

doi: 10.13332/j.cnki.jbfu.2015.01.017
基金项目: 

国家自然科学基金项目(31170168)、国家星火计划项目(2013GA651006)、国家火炬计划项目(2012GH531899)、中央高校自主科研基金项目(DC120101143、DC120140472)、博士启动基金项目(20120914)。

详细信息
    作者简介:

    第一作者:郭鹏,博士,讲师。主要研究方向:抗逆分子生物学。Email: gp@dlnu.edu.cn 地址:116600辽宁省大连开发区辽河西路18号大连民族学院环境与资源学院。
    责任作者: 姜健,教授。主要研究方向:植物生理与生物技术。Email: jix@dlnu.edu.cn 地址:同上

    第一作者:郭鹏,博士,讲师。主要研究方向:抗逆分子生物学。Email: gp@dlnu.edu.cn 地址:116600辽宁省大连开发区辽河西路18号大连民族学院环境与资源学院。
    责任作者: 姜健,教授。主要研究方向:植物生理与生物技术。Email: jix@dlnu.edu.cn 地址:同上

Cloning and characterization of PdDHN2b gene from Populus deltoides×Populus nigra.

  • 摘要: 以欧美杨为材料,通过RT-PCR方法从欧美杨叶片中克隆到了抗旱脱水素DHN2b基因(命名为PdDHN2b),该基因开放阅读框为1 440 bp,编码379个氨基酸,绝大多数氨基酸组成属于亲水性氨基酸,无明显跨膜结构域。亚细胞定位表明PdDHN2b基因定位在细胞质或细胞膜上。荧光定量PCR分析表明:该基因在顶端中表达量最高,功能叶、根和茎中则无表达。同时该基因受NaCl、ABA和干旱等诱导表达。另外,构建了原核表达载体pET-PdDHN2b,并在大肠杆菌BL21中表达融合蛋白,诱导纯化后免疫小白鼠,制备抗体。SDS-PAGE电泳结果表明,融合蛋白分子量为58 ku。最后利用Ni-NTA纯化的PdDHN2b-his重组蛋白用于Western blot分析。

     

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出版历程
  • 收稿日期:  2013-12-24
  • 刊出日期:  2015-01-30

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