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4种检测柠条种子活力技术的比较分析

李加国 李松林 韩梅 汪晓峰

李加国, 李松林, 韩梅, 汪晓峰. 4种检测柠条种子活力技术的比较分析[J]. 北京林业大学学报, 2015, 37(1): 15-28. doi: 10.13332/j.cnki.jbfu.2015.01.020
引用本文: 李加国, 李松林, 韩梅, 汪晓峰. 4种检测柠条种子活力技术的比较分析[J]. 北京林业大学学报, 2015, 37(1): 15-28. doi: 10.13332/j.cnki.jbfu.2015.01.020
LI Jia-guo, LI Song-lin, HAN Mei, WANG Xiao-feng. Comparative analysis of four techniques for evaluating seed viability of Caragana korshinskii Kom.[J]. Journal of Beijing Forestry University, 2015, 37(1): 15-28. doi: 10.13332/j.cnki.jbfu.2015.01.020
Citation: LI Jia-guo, LI Song-lin, HAN Mei, WANG Xiao-feng. Comparative analysis of four techniques for evaluating seed viability of Caragana korshinskii Kom.[J]. Journal of Beijing Forestry University, 2015, 37(1): 15-28. doi: 10.13332/j.cnki.jbfu.2015.01.020

4种检测柠条种子活力技术的比较分析

doi: 10.13332/j.cnki.jbfu.2015.01.020
基金项目: 

“948”国家林业局引进项目(2011-- 4-- 54)、林业公益性行业科研专项(201104024)。

详细信息
    作者简介:

    第一作者:李加国。主要研究方向:种子生物学。Email:lijiaguo2011@sina.com 地址:100083 北京市清华东路35号北京林业大学生物科学与技术学院。
    责任作者: 汪晓峰,教授。主要研究方向:种子生物学。Email: wxf801@sina.com 地址:同上。

    第一作者:李加国。主要研究方向:种子生物学。Email:lijiaguo2011@sina.com 地址:100083 北京市清华东路35号北京林业大学生物科学与技术学院。
    责任作者: 汪晓峰,教授。主要研究方向:种子生物学。Email: wxf801@sina.com 地址:同上。

Comparative analysis of four techniques for evaluating seed viability of Caragana korshinskii Kom.

  • 摘要: 种子保存是当前植物种质资源保存的最有效、最安全的方式之一,快速、微量、准确检测种子库中种子活力的变化是当前植物种质设施保存技术研究的热点与重点。本文利用谷胱甘肽氧化还原电位(EGSSG/2GSH)、差示扫描量热分析(DSC)、电子自旋共振(EPR)和非损伤微检测(NMT)4种目前较为先进的方法,研究柠条种子在人工老化下的活力变化规律。结果表明,柠条种子在50 ℃、相对湿度(RH)为50%的老化条件下,其活力指数的下降速率要快于发芽率的下降速率;通过建立回归模型发现,在检测发芽率变化方面,4种检测方法的优越性依次为EPRNMTDSCEGSSG/2GSH;在检测活力指数变化方面,4种检测方法的优越性依次为NMTEGSSG/2GSH=EPRDSC。综合对检测柠条种子发芽率和活力指数拟合度情况,以及对种子非损伤的性质, 认为NMT最适合用来跟踪监测贮藏种子的活力。

     

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出版历程
  • 收稿日期:  2014-01-02
  • 刊出日期:  2015-01-30

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