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铜绿假单胞菌与长枝木霉对杂交竹梢枯病的协同增效生防研究

谯天敏 张静 朱天辉

谯天敏, 张静, 朱天辉. 铜绿假单胞菌与长枝木霉对杂交竹梢枯病的协同增效生防研究[J]. 北京林业大学学报, 2015, 37(2): 113-120. doi: 10.13332/j.cnki.jbfu.2015.02.012
引用本文: 谯天敏, 张静, 朱天辉. 铜绿假单胞菌与长枝木霉对杂交竹梢枯病的协同增效生防研究[J]. 北京林业大学学报, 2015, 37(2): 113-120. doi: 10.13332/j.cnki.jbfu.2015.02.012
QIAO Tian-min, ZHANG Jing, ZHU Tian-hui. Synergistic biocontrol mechanism of Pseudomonas aeruginosa and Trichoderma longibrachiatum on Arthrinium phaeospermum[J]. Journal of Beijing Forestry University, 2015, 37(2): 113-120. doi: 10.13332/j.cnki.jbfu.2015.02.012
Citation: QIAO Tian-min, ZHANG Jing, ZHU Tian-hui. Synergistic biocontrol mechanism of Pseudomonas aeruginosa and Trichoderma longibrachiatum on Arthrinium phaeospermum[J]. Journal of Beijing Forestry University, 2015, 37(2): 113-120. doi: 10.13332/j.cnki.jbfu.2015.02.012

铜绿假单胞菌与长枝木霉对杂交竹梢枯病的协同增效生防研究

doi: 10.13332/j.cnki.jbfu.2015.02.012
基金项目: 

国家自然科学基金项目(5DKA21207-13)。

详细信息
    责任作者:

    第一作者: 谯天敏,博士,副研究员。 主要研究方向:林木病害及生防教学。 Email: 525636693@qq.com 地址:625014 四川省雅安市雨城区新康路46号四川农业大学林学院。 责任作者: 朱天辉,教授,博士生导师。主要研究方向:林木病害及生防教学。 Email: zhuth1227@126.com 地址:同上。

    第一作者: 谯天敏,博士,副研究员。 主要研究方向:林木病害及生防教学。 Email: 525636693@qq.com 地址:625014 四川省雅安市雨城区新康路46号四川农业大学林学院。 责任作者: 朱天辉,教授,博士生导师。主要研究方向:林木病害及生防教学。 Email: zhuth1227@126.com 地址:同上。

Synergistic biocontrol mechanism of Pseudomonas aeruginosa and Trichoderma longibrachiatum on Arthrinium phaeospermum

  • 摘要: 铜绿假单胞菌和长枝木霉均对杂交竹梢枯病菌具有明显的拮抗作用。通过室内抗生素、紫外诱变和杀菌剂胁迫培养,获得铜绿假单胞菌和长枝木霉突变菌株,此突变体在形态特征、平板抑菌活性上与原始菌株差异不显著,且具有长期稳定的抗药性。田间喷施突变菌株菌悬液或孢子悬液可以使其成功定殖到杂交竹叶表面。铜绿假单胞菌和长枝木霉的亲和性研究显示,二者浓度低于108 cfu/mL,均无明显抑制作用,具有高度亲和性,这有利于2菌株在杂交竹梢枯病生物防治中协同作用的发挥。利用铜绿假单胞菌悬液和长枝木霉孢悬液进行梢枯病菌田间协同防治试验,结果表明:预先接种拮抗菌防效最高,其次是同时接种,后施拮抗菌防效最低,说明铜绿假单胞菌和长枝木霉均有良好的防病作用。另外,2菌株的联合使用在3种处理中防效均最优,尤其是在先接种病原菌的处理中,协同防效得到很大提高,说明二者联合使用有良好的梢枯病治理作用。

     

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  • 收稿日期:  2014-05-08

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