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欧美杨PdPP2C基因的克隆与功能分析

郭鹏 张士刚 邢鑫 姜健

郭鹏, 张士刚, 邢鑫, 姜健. 欧美杨PdPP2C基因的克隆与功能分析[J]. 北京林业大学学报, 2015, 37(2): 100-106. doi: 10.13332/j.cnki.jbfu.2015.02.018
引用本文: 郭鹏, 张士刚, 邢鑫, 姜健. 欧美杨PdPP2C基因的克隆与功能分析[J]. 北京林业大学学报, 2015, 37(2): 100-106. doi: 10.13332/j.cnki.jbfu.2015.02.018
GUO Peng, ZHANG Shi-gang, XING Xin, JIANG Jian. Cloning and function analysis of PdPP2C from Populus deltoides[J]. Journal of Beijing Forestry University, 2015, 37(2): 100-106. doi: 10.13332/j.cnki.jbfu.2015.02.018
Citation: GUO Peng, ZHANG Shi-gang, XING Xin, JIANG Jian. Cloning and function analysis of PdPP2C from Populus deltoides[J]. Journal of Beijing Forestry University, 2015, 37(2): 100-106. doi: 10.13332/j.cnki.jbfu.2015.02.018

欧美杨PdPP2C基因的克隆与功能分析

doi: 10.13332/j.cnki.jbfu.2015.02.018
基金项目: 

国家自然科学基金项目(31170168)、国家星火计划项目(2013GA651006)、国家火炬计划项目(2012GH531899)、中央高校自主科研基金项目(DC120101143)、博士启动基金项目(20120914)。

详细信息
    责任作者:

    第一作者: 郭鹏,博士,讲师。主要研究方向:抗逆分子生物学。Email: gp@dlnu.edu.cn 地址:辽宁省大连开发区辽河西路18号大连民族学院环境与资源学院。 责任作者: 姜健,教授。主要研究方向:植物生理与生物技术。Email: jix@dlnu.edu.cn 地址:同上。

    第一作者: 郭鹏,博士,讲师。主要研究方向:抗逆分子生物学。Email: gp@dlnu.edu.cn 地址:辽宁省大连开发区辽河西路18号大连民族学院环境与资源学院。 责任作者: 姜健,教授。主要研究方向:植物生理与生物技术。Email: jix@dlnu.edu.cn 地址:同上。

Cloning and function analysis of PdPP2C from Populus deltoides

  • 摘要: 欧美杨是中纬度地区最适合的短轮伐期工业用材集约经营树种之一,然而盐渍、干旱和低温严重影响了欧美杨的生长发育和产量。本文利用RT-PCR等技术克隆出欧美杨PP2C基因并进行了序列分析。PdPP2C基因开放阅读框为1 320 bp,编码439个氨基酸。蛋白质序列分析表明,PP2C蛋白N端保守性不强,C端有保守的蛋白磷酸酶区域。荧光定量PCR分析表明,该基因在欧美杨根、成熟叶、顶端叶和茎中都有表达且根中表达量最高。逆境胁迫分析表明,该基因受NaCl、ABA和干旱等诱导表达。干旱和盐胁迫处理下转PdPP2C基因拟南芥与野生型相比生长受到较少抑制,且叶绿素含量上升,丙二醛含量下降,表明PdPP2C基因作为一种蛋白磷酸酶提高了拟南芥的抗逆性。

     

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  • 收稿日期:  2014-01-08

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