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    邓文红, 张风娟, 金幼菊. 反相高效液相色谱法测定植物组织中的水杨酸[J]. 北京林业大学学报, 2007, 29(1): 151-154. DOI: 10.13332/j.1000-1522.2007.01.028
    引用本文: 邓文红, 张风娟, 金幼菊. 反相高效液相色谱法测定植物组织中的水杨酸[J]. 北京林业大学学报, 2007, 29(1): 151-154. DOI: 10.13332/j.1000-1522.2007.01.028
    shu
    DENG Wen-hong, ZHANG Feng-juan, JIN You-ju. Determination of salicylic acid in the plant tissues using RP-HPLC[J]. Journal of Beijing Forestry University, 2007, 29(1): 151-154. DOI: 10.13332/j.1000-1522.2007.01.028
    Citation: DENG Wen-hong, ZHANG Feng-juan, JIN You-ju. Determination of salicylic acid in the plant tissues using RP-HPLC[J]. Journal of Beijing Forestry University, 2007, 29(1): 151-154. DOI: 10.13332/j.1000-1522.2007.01.028
    shu

    反相高效液相色谱法测定植物组织中的水杨酸

    Determination of salicylic acid in the plant tissues using RP-HPLC

      摘要
    • 摘要: 以五角枫叶片为实验材料，经预处理后用反相高效液相色谱测定了植物组织中水杨酸的含量.该方法摸索出了使水杨酸与其他酸性（或杂质）组分很好分离的液相色谱条件，建立了植物组织中水杨酸的定性定量方法.用该方法测定水杨酸标样的回收率高于95%，精密度和重现性均较好（相对标准偏差为0.42%）.用该方法对实际植物样品的分析也取得了较理想的结果.

       

      Abstract: Salicylic acid(SA) in the leaves of Acer mono was detected by reverse phase high performance liquid chromatography(RP-HPLC) after the tissues were pretreated.The analysis method for SA in the plant tissues was established.The recycle rate of this method was higher than 95%.Its relative standard deviation(RSD) was 0.42%.The results showed that SA was well separated from other acidic interferents, and the ideal quantitative data of SA in A.mono were obtained.

       

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