Abstract: In order to develop the genetic transformation,tissue culture of Lespedeza cuneata was studied in this paper.The results indicated that the best time of seed disinfectant of 2.1% NaClO was 10 min,and the pollution percentage was zero under this sterilization condition.The optimal concentrations of primary differentiation were 6-BA 1.0-2.0 mg/L,NAA 0.01 mg/L and 2,4-D 0-0.01 mg/L,on which the differentiation index could reach 2-2.5.The optimal culture medium in secondary differentiation was MS medium containing original macroelements+6-BA 2.0 mg/L+IBA 0.5 mg/L+2,4-D 0.5 mg/L.IBA was more suitable than NAA for the rooting of plantlets.The optimum concentration of IBA was 0.5-1.0 mg/L,whereas that of NAA should be less than 0.1 mg/L,since high concentration of NAA had an inhibiting effect on rooting.In order to solve the serious problem of bud etiolation,experiments with different concentrations of NH₄NO₃,MgSO₄·7H₂O,CaCl₂·2H₂O,iron salt,sugar and types of lights were conducted.The results showed that the concentration changes of N,Mg and Fe had significant effects on inhibiting etiolation,and the optimal concentrations were NH₄NO₃ 1 600 mg/L,MgSO₄·7H₂O 300 mg/L,and iron salt 83.4 mg/L.Another three factors had no significant effects on etiolation.And there were no significant differences in the generation index for all of the six factors in the etiolation experiments.