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    谭健晖, 王以红, 陈学政, 蔡玲, 朱高浦. 桉树无性繁殖衰退过程中的生理变化[J]. 北京林业大学学报, 2007, 29(3): 15-22. DOI: 10.13332/j.1000-1522.2007.03.003
    引用本文: 谭健晖, 王以红, 陈学政, 蔡玲, 朱高浦. 桉树无性繁殖衰退过程中的生理变化[J]. 北京林业大学学报, 2007, 29(3): 15-22. DOI: 10.13332/j.1000-1522.2007.03.003
    TAN Jian-hui, WANG Yi-hong, CHEN Xue-zheng, CAI Ling, ZHU Gao-pu. Physiological changes during the senescence process of vegetative propagations of eucalyptus[J]. Journal of Beijing Forestry University, 2007, 29(3): 15-22. DOI: 10.13332/j.1000-1522.2007.03.003
    Citation: TAN Jian-hui, WANG Yi-hong, CHEN Xue-zheng, CAI Ling, ZHU Gao-pu. Physiological changes during the senescence process of vegetative propagations of eucalyptus[J]. Journal of Beijing Forestry University, 2007, 29(3): 15-22. DOI: 10.13332/j.1000-1522.2007.03.003

    桉树无性繁殖衰退过程中的生理变化

    Physiological changes during the senescence process of vegetative propagations of eucalyptus

    • 摘要: 以桉树不同无性繁殖方法及不同繁殖代数的幼树叶片为材料,对其光合代谢、氮代谢、内源保护酶系统以及膜脂反应产物等生理指标进行分析和对比,探讨桉树不同繁殖方法及繁殖代数衰退过程中的变化规律和差异.结果表明:①除游离脯氨酸(PRO)含量外,不同无性繁殖方法及不同繁殖代数的叶绿素含量、可溶性蛋白质含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、质膜过氧化的产物丙二醛(MDA)含量等6个生理指标均具显著差异.②随着衰老的加剧和时间的延长,叶绿素含量、可溶性蛋白质含量和CAT活性呈下降的趋势;SOD活性总体上呈下降的趋势,但不同处理降到最低点的树龄不同;POD活性变化不一致,总体呈先下降再升高的趋势;而MDA含量、PRO含量呈先升高后降低再升高的趋势.③叶绿素含量、可溶性蛋白质含量、SOD活性、POD活性、MDA含量能稳定、准确地反映桉树抗衰退的能力,以上5个指标与桉树衰老具有明显的相关性.叶绿素含量、可溶性蛋白质含量、SOD活性与抗衰老呈正相关,数值越高,表明抗衰退的能力越强;POD活性、MDA含量与抗衰老呈负相关,数值越高,表明抗衰退的能力越弱;CAT活性、PRO含量与桉树衰老的关系无规律性变化,不宜采用其评价桉树抗衰退的强弱.④综合叶片的7个生理指标对桉树无性繁殖方法和繁殖代数进行评价,组培苗抗衰退能力强于扦插苗,随着繁殖代数的增加抗衰退的能力下降,扦插苗的衰退速度大于组培苗,组培1代抗衰退能力最强,组培8代次之,3年扦插最弱.建议生产上定期对繁殖材料进行复壮更新,确保无性繁殖的可靠性和稳定性.

       

      Abstract: The young trees of eucalyptus built by different vegetative propagation methods and varied generations were taken as the experimental materials.And their photosynthetic metabolism,nitrogen metabolism,endogenous protected enzyme system,membrane lipid reacting offspring,etc.were tested and compared to explore the rules and differences of variety on eucalyptus senescence with different propagation methods and generations.The results showed that:1) the indices had obvious differences in chlorophyll content,soluble protein content,SOD activity,POD activity,CAT activity,MDA content of different vegetative propagation methods and generations except PRO content.2) According to the research,the testing results showed that with the increase of senescence,the chlorophyll and soluble protein content of leaves decreased;the activity of SOD decreased in the mass and its minimum activity caused by different treatments would appear in different tree ages;the activity of CAT decreased and the activity of POD showed descending trend at first and then ascended;while the content of MDA showed the trend of rising-declining-rising;the content of dissociative proline increased at first and then decreased.3) Chlorophyll content,soluble protein content,SOD activity,POD activity and MDA content could explore the anti-senescence ability of eucalyptus stably and accurately;the five indices above had obvious correlations with the senescence of eucalyptus;chlorophyll content,soluble protein content and SOD activity had positive relationship with the anti-senescence ability of eucalyptus,ie the more the numerical values were,the stronger the anti-senescence ability was; while the POD activity and MDA content had negative relationship with the anti-senescence ability of eucalyptus,ie the more the values were,the weaker the anti-senescence ability was;CAD activity and PRO content were irrelated with the senescence of eucalyptus,thus both of them couldn't be used to evaluate the anti-senescence ability of eucalyptus.4) According to the above testing results by the seven physiological indices,the resistance to senescence of the first tissue culture generation was the strongest,the eighth tissue culture generation was second,and the third generation from cutting was the weakest.It is suggested that the materials of vegetative propagation would be renovated and renewed periodically during production to ensure the reliability and stability of vegetative propagation.

       

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