铜、镉胁迫对舞毒蛾排毒代谢酶的影响

张凯; 周艳涛; 王皙玮; 姜虹; 王嘉冰; 张文一; 严善春

doi:10.13332/j.1000-1522.20150293

铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

1.
1 东北林业大学林学院
2.
2 吉林省长春市园林植物保护站
3.
3 国家林业局森林病虫害防治总站

基金项目:

东北林业大学学术名师支持计划项目(010602071)。

详细信息

作者简介:
张凯。主要研究方向:昆虫化学生态学。Email:1020654381@qq.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 严善春,教授,博士生导师。主要研究方向:昆虫化学生态学。Email:yanshanchun@126.com 地址:同上。

张凯。主要研究方向:昆虫化学生态学。Email:1020654381@qq.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 严善春,教授,博士生导师。主要研究方向:昆虫化学生态学。Email:yanshanchun@126.com 地址:同上。

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

1.
1 College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P. R. China;
2.
2 Changchun Landscape Plant Protection Station, Changchun, Jilin, 130022, P. R. China;
3.
3 General Station of Forest Pest Management, State Forestry Administration, Shenyang, Liaoning, 110034, P. R. China

摘要: 为了探讨重金属铜、镉污染胁迫下舞毒蛾体内排毒代谢酶活性的变化,本文以3种不同质量分数的铜(Cu,400、700、1 000 mg/kg)、镉(Cd, 1.0、3.0、6.0 mg/kg)处理土壤,用处理后的土壤盆栽1年生小黑杨扦插苗,研究舞毒蛾体内排毒代谢酶活性的变化趋势。结果发现,Cu、Cd不同浓度处理的杨树对舞毒蛾3龄、4龄幼虫体内排毒代谢酶活性有显著的干扰作用。与对照组相比,中、高质量分数Cu、Cd处理组3龄幼虫体内SOD、ACP和AKP酶活性显著降低(P0.05),低质量分数Cu、Cd处理组下降不显著(P0.05)。而POD和GSTs酶活性变化恰恰相反,中、高质量分数Cu、Cd处理组舞毒蛾3龄幼虫体内POD和GSTs酶活性显著升高(P0.05),低质量分数Cu、Cd处理升高不显著(P0.05)。中、高质量分数Cu、Cd处理下,4龄幼虫体内SOD、POD、ACP、AKP和GSTs酶活性和对照组相比显著降低(P0.05),低质量分数重金属处理和对照组相比下降不显著(P0.05)。以上结果显示,中、高质量分数Cu、Cd胁迫能显著干扰舞毒蛾幼虫的排毒代谢,且随着幼虫由3龄到4龄的发育,最终均表现为显著的抑制作用;低质量分数Cu、Cd胁迫对舞毒蛾幼虫排毒代谢干扰作用不显著。
- 重金属胁迫 /
- 小黑杨 /
- 舞毒蛾 /
- 排毒代谢酶
Abstract: In order to investigate the changes of detoxification metabolic activity in gypsy moth (Lymantria dispar) under the heavy metal pollution stress, we measured the detoxifying metabolism in gypsy moth body feeding on one-year potted seedlings of Populus simonii×P. nigra after treating the soil with three concentrations of copper (Cu, 400, 700, 1 000 mg/kg) and cadmium (Cd, 1.0, 3.0, 6.0 mg/kg). The results showed that Cu and Cd had a significant impact on the detoxification metabolic enzyme in the third and fourth instar larvae. The activities of SOD, ACP and AKP enzymes in vivo in the third instar larvae at medium and high concentrations of Cu and Cd were lower than the control group (P0.05),but at the low concentrations of Cu and Cd, the difference was not obvious (P0.05). The change of activities of POD and GSTs was just the opposite. The activities of POD and GSTs enzymes in the third instar larvae at medium and high concentrations of Cu and Cd increased significantly (P0.05), but not obvious (P0.05) at the low concentrations of Cu and Cd. The activities of SOD, POD, ACP, AKP and GSTs enzymes in the fourth instar larvae at medium and high concentrations of Cu and Cd decreased significantly (P0.05),but not obvious at the low concentrations of Cu and Cd treatment (P0.05). These results showed that the high levels of Cu and Cd stress could significantly interfere with the metabolic detoxification of gypsy moth larvae, and with the third instar larvae developing into the fourth instar, the heavy metal stress finally showed a significant inhibition to the development of larvae, while low levels of Cu and Cd stress had not significant interference with metabolic detoxification of the larvae.
- heavy metal stress /
- Populus simonii×P. nigra /
- Lymantria dispar /
- detoxifying metabolic enzyme

[1]	GAO M, JIN L, GUO X. A study of hazards of soil heavy metals Cu stress on plants[J]. Open Journal of Nature Science, 2014, 2: 18-23.
[2]	HU M M, WANG Q, YANG Y Z. Accumulation, distribution and excretion of heavy metals in insects[J]. Chinese Agricultural Science Bulletin, 2012, 28(18):213-217.
[3]	DING P, ZHUANG P, LI Z, et al. Accumulation and detoxification of cadmium by larvae of Prodenia litura (Lepidoptera: Noctuidae) feeding on Cd-enriched amaranth leaves[J]. Chemosphere, 2013, 91(1): 28-34.
[4]	YANG S Y, HUANG Y J, ZHANG M, et al. Ecophysiological effects of heavy metals on insects[J]. Acta Entomologica Sinica, 2015,58(4):427-436.
[5]	WU Q X, XIA Q. Effect of heavy metal on antioxidant enzymes in insects[J].Journal of Environmental Entomology,2014,36(2): 247-251.
[6]	LANNONE M F, GROPPA M D, BENAVIDES M P. Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants[J]. Environmental and Experimental Botany, 2015, 109: 201-211.
[7]	WANG H, WU G X, YE G Y, et al. Accumulation of cuprum and cadmium and their effects on the antioxidant enzymes in Boettcherisca peregrine exposed to cuprum and cadmium[J]. Journal of Zhejiang University(Agric Life Sci),2006,32(1):77-81.
[8]	LI S H, ZHANG G J, GAO W J, et al. Plant growth, development and change in GSH level in safflower (Carthamus tinctorius L.) exposed to copper and lead[J]. Archives of Biological Sciences, 2015, 1: 6.
[9]	GAO H H. Defense mechanism of Sitobion avenae (Hemiptera: Aphididae) under the long-term exposure of cadmium and zinc[D]. Yangling: Northwest A F University, 2013.
[10]	胡蒙蒙, 王青, 杨益众. 重金属在昆虫体内的累积、分布与排泄[J]. 中国农学通报, 2012, 28(18): 213-217.
[11]	NIU Y Q, ZHENG J F, WANG H L. Progress in effect of lead on anti-oxidative damage enzyme of aquatic animal[J]. Environmental Science and Technology,2010(2):74-78.
[12]	杨世勇, 黄永杰, 张敏, 等. 重金属对昆虫的生态生理效应[J].昆虫学报, 2015, 58(4): 427-436.
[13]	LIU W G, ZHANG X D, HUANG L L, et al. Research progress on physiologic and ecologic characteristics of poplar[J].World Forestry Research, 2010,23(1):50-55.
[14]	吴启仙, 夏嫱. 重金属对昆虫抗氧化酶影响研究进展[J]. 环境昆虫学报, 2014, 36(2): 247-251.
[15]	王慧, 吴国星, 叶恭银, 等. 铜和镉在棕尾别麻蝇体内的累积及其对三种抗氧化酶活性的影响[J]. 浙江大学学报(农业与生命科学版), 2006, 32(1): 77-81.
[16]	Environmental quality standard for soils: GB15618[S]. Beijing: China Standard Press,1995.
[17]	MENG Z H, ZHOU J, ZHENG Y F. Ecological risk assessment of heavy metal elements in urban soil of Harbin[J]. Research of Soil and Water Conservation,2009,16(2):153-159.
[18]	高欢欢. 重金属Cd和Zn长期胁迫下麦长管蚜Sitobion avenae防御机制的研究[D]. 杨凌:西北农林科技大学, 2013.
[19]	LU Y F, YAN J X, LI S W, et al. Effects of the Larix gmelinii grown under different light intensities on the development and defensive enzyme activities of Lymantria dispar larvae[J]. Acta Ecologica Sinica, 2013,33(22):7125-7131.
[20]	MIRACˇU2IAC＇U2 D, BLAGOJEVIAC＇U2 D, PERIAC＇U2-MATARUGA V, et al. Cadmium effects on the fitness-related traitsand antioxidative defense of Lymantria dispar L. larvae[J].Environ Sci Pollut Res, 2013, 20: 209-218.
[21]	牛亚青, 郑济芳, 王晗莉. 重金属铅对水生动物抗氧化损伤酶的影响研究进展[J]. 环境科技, 2010(2):74-78.
[22]	KONG X P, LI S W, CUI W C, et al. Effects of different light intensities on the development and defensive enzyme activities of Lymantria dispar[J]. Journal of Northeast Forestry University,2013,41(7):104-107.
[23]	刘文国, 张旭东, 黄玲玲, 等. 我国杨树生理生态研究进展[J]. 世界林业研究, 2010, 23(1):50-55.
[24]	YAN J M, LIAO Y Z, YAN S C, et al. Effects of methoxyfenozide on the activities of detoxifying enzymes and protective enzymes in Lymantria dispar (Lepidoptera: Lymantriidae)[J]. Journal of Northeast Forestry University,2010,38(11):112-114.
[25]	ROBERT L H, MELODY A K, DANIEL L R. Classification, genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia, Europe, and North America[J]. Journal of Invertebrate Pathology, 2014, 116:27-35.
[26]	FENG C F, YAN S C, LU Y F, et al. Effects of induced resistance of Larix gmelinii on the activities of detoxifying enzymes in Lymantria dispar[J]. Scientia Silvae Sinicae,2011,47(8):102-107.
[27]	中华人民共和国国家标准土壤环境质量标准:GB15618[S]. 北京: 中国标准出版社, 1995.
[28]	LI H, YAN S C, CAO C W. Effects of furan tebufenozide on activities of detoxifying enzymes of Lymantria dispa[J].Journal of Northeast Forestry University,2011,39(9):97-100.
[29]	孟昭虹, 周嘉, 郑元福. 哈尔滨市城市土壤重金属生态风险评价[J]. 水土保持研究, 2009, 16(2): 153-159.
[30]	WANG Y, LI L J, ZHANG Y P, et al. Accumulation of chromium in Oxya chinensis and its effects on the antioxidant system[J]. Journal of Agro-Environment Science,2010,29(12):2281-2286.
[31]	LIU Y M, YANG H M, ZHANG Y P, et al. Acute effects of Cd2+ and Cr6+ on detoxification enzymes and polyphenol oxidase in Oxya chinensis[J]. Journal of Agro-Environment Science, 2013,32(7):1321-1327.
[32]	鲁艺芳, 严俊鑫, 李霜雯, 等. 不同光照强度下兴安落叶松对舞毒蛾幼虫生长发育及防御酶的影响[J]. 生态学报, 2013, 33(22): 7125-7131.
[33]	孔祥鹏, 李霜雯, 崔伟婵, 等. 不同光照强度对舞毒蛾生长发育及防御酶活性的影响[J]. 东北林业大学学报, 2013, 41(7): 104-107.
[34]	HAO R Z, YU Y, WU J Y, et al. Research progress on mechanisms of metal resistance in trees[J]. Chinese Agricultural Science Bulletin, 2012,28(10):6-12.
[35]	ZHANG K, XU B, MENG Z J, et al. Effect of copper and cadmium on defensive protein activity in poplar leaves[J]. Journal of Northeast Forestry University,2014,42(11):43-46.
[36]	鄢杰明, 廖月枝, 严善春, 等. 甲氧虫酰肼对舞毒蛾解毒酶和保护酶活性的影响[J]. 东北林业大学学报, 2010, 38(11): 112-114.
[37]	冯春富, 严善春, 鲁艺芳, 等. 兴安落叶松诱导抗性对舞毒蛾幼虫解毒酶活性的影响[J]. 林业科学, 2011, 47(8): 102-107.
[38]	李慧, 严善春, 曹传旺. 呋喃虫酰肼对舞毒蛾解毒酶活力的影响[J]. 东北林业大学学报, 2011, 39(9): 97-100.
[39]	BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical biochemistry, 1976, 72(1): 248-254.
[40]	王跃, 李丽君, 张育平, 等. 铬在中华稻蝗(Oxya chinensis)体内的累积及对抗氧化系统的影响[J]. 农业环境科学学报, 2010, 29(12): 2281-2286.
[41]	刘耀明, 杨慧敏, 张育平, 等. 镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响[J]. 农业环境科学学报, 2013, 32(7): 1321-1327.
[42]	WU G Q, YI Y H. Effects of dietary heavy metals on the immune and antioxidant systems of Galleria mellonella larvae[J]. Comparative Biochemistry and Physiology, 2015,167:131-139.
[43]	郝瑞芝, 余洋, 武佳叶, 等. 树木对重金属的抗性机理研究进展[J]. 中国农学通报, 2012, 28(10): 6-12.
[44]	张凯, 徐波, 孟昭君, 等. 铜、镉胁迫对杨树叶片中防御蛋白活性的影响[J]. 东北林业大学学报, 2014, 42(11):43-46.

[1]	王雪怡, 顾咏梅, 张雪梅, 姜廷波, 刘焕臻. 杨树HSF家族基因生物信息学与胁迫应答表达分析 . 北京林业大学学报, 2021, 43(2): 34-45. doi: 10.12171/j.1000-1522.20200159
[2]	李欣悦, 王振越, 曹传旺. LdNPV对CO₂胁迫下舞毒蛾生长发育及生化酶活性影响 . 北京林业大学学报, 2019, 41(9): 139-146. doi: 10.13332/j.1000-1522.20180199
[3]	宋博, 李凤日, 董利虎, 周翼飞. 黑龙江西部地区人工小黑杨立木可加性生物量模型 . 北京林业大学学报, 2018, 40(11): 58-68. doi: 10.13332/j.1000-1522.20180062
[4]	刘鹏, 孙丽丽, 张琪慧, 曹传旺. 舞毒蛾小分子热激蛋白基因分析及对甲萘威胁迫的响应 . 北京林业大学学报, 2017, 39(1): 78-84. doi: 10.13332/j.1000-1522.20160179
[5]	王亚军, 邹传山, 王若茜, 林连男, 张国财. 3种植物次生代谢物质对舞毒蛾的杀虫活性分析 . 北京林业大学学报, 2017, 39(11): 75-81. doi: 10.13332/j.1000-1522.20170214
[6]	王晓佳, 王百田, 李德宁, 曹远博. 聚丙稀酰胺高吸附树脂与重金属离子的相互作用 . 北京林业大学学报, 2016, 38(3): 81-88. doi: 10.13332/j.1000-1522.20150362
[7]	孙丽丽, 刘鹏, 王志英, 梁斌, 曹传旺. 转LdOA1基因果蝇品系GSTs基因表达及对溴氰菊酯胁迫响应 . 北京林业大学学报, 2016, 38(6): 72-78. doi: 10.13332/j.1000-1522.20150418
[8]	赵慧, 王遂, 姜静, 刘桂丰. 酵母双杂交筛选与小黑杨PsnWRKY70相互作用的蛋白质 . 北京林业大学学报, 2016, 38(2): 44-51. doi: 10.13332/j.1000-1522.20150162
[9]	姜礅, 王杰, 姜虹, 张文一, 孟昭军, 严善春. 茉莉酸甲酯局部诱导长白落叶松对舞毒蛾生长发育的影响 . 北京林业大学学报, 2016, 38(6): 67-71. doi: 10.13332/j.1000-1522.20150493
[10]	曲赞霜, 钱婷婷, 侯聪, 张力杰, 魏志刚. 小黑杨碳酸酐酶家族基因表达特性分析 . 北京林业大学学报, 2015, 37(2): 94-99. doi: 10.13332/j.cnki.jbfu.2015.02.016
[11]	邹建美, 孙江, 戴伟, 姚志斌, 杨添, 刘娜丽. 北京近郊耕作土壤重金属状况评价分析 . 北京林业大学学报, 2013, 35(1): 132-138.
[12]	李志新, 赵曦阳, 杨成君, 汪广宇, 王福森, 张连飞, 张连才, 刘桂丰, 姜静. 转TaLEA基因小黑杨株系变异及生长稳定性分析 . 北京林业大学学报, 2013, 35(2): 57-62.
[13]	李慧, 严善春, 曹传旺, 李奇. 呋喃虫酰肼对舞毒蛾生长发育和保护酶活性的影响 . 北京林业大学学报, 2012, 34(2): 96-102.
[14]	鄢杰明, 严善春, 曹传旺. 多杀菌素对舞毒蛾保护酶及几丁质酶的影响 . 北京林业大学学报, 2012, 34(1): 80-85.
[15]	张凯旋, 赵桂媛, 刘关君, 刘桂丰, 杨传平, 魏志刚. 小黑杨应拉木上下侧cDNA文库的比较分析 . 北京林业大学学报, 2011, 33(3): 8-13.
[16]	李菁, 骆有庆, 石娟, 周娇, 王先礼, 马凌云, 陈超. 利用植物源引诱剂监测与控制舞毒蛾 . 北京林业大学学报, 2011, 33(4): 85-90.
[17]	胡晓, 严善春, 鲁艺芳, 刘彤. 东北红豆杉次生代谢物对舞毒蛾拒食活性的影响 . 北京林业大学学报, 2011, 33(5): 151-154.
[18]	王爱霞, 张敏, 方炎明, 贾恒, 周楠楠. 行道树对重金属污染的响应及其功能型分组 . 北京林业大学学报, 2010, 32(2): 177-183.
[19]	赵桂媛, 魏志刚, 刘关君, 张凯旋, 刘桂丰, 杨传平. 策略构建小黑杨茎形成层全长cDNA文库 . 北京林业大学学报, 2010, 32(1): 52-56.
[20]	张茹琴, 唐明, 张峰峰, 黄继川. 酸碱度和重金属对3种外生菌根真菌生长的影响 . 北京林业大学学报, 2008, 30(2): 113-118.

点击查看大图

计量

文章访问数: 623
HTML全文浏览量: 50
PDF下载量: 12
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板

铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

计量

铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

1. 1 东北林业大学林学院

2. 2 吉林省长春市园林植物保护站

3. 3 国家林业局森林病虫害防治总站

English Abstract

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

全文HTML

目录

留言板

铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

计量

出版历程

铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

1. 1 东北林业大学林学院 2. 2 吉林省长春市园林植物保护站 3. 3 国家林业局森林病虫害防治总站

English Abstract

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

全文HTML

目录

1. 1 东北林业大学林学院

2. 2 吉林省长春市园林植物保护站

3. 3 国家林业局森林病虫害防治总站