铜、镉胁迫对舞毒蛾排毒代谢酶的影响

张凯; 周艳涛; 王皙玮; 姜虹; 王嘉冰; 张文一; 严善春

doi:10.13332/j.1000-1522.20150293

铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

1.
1 东北林业大学林学院
2.
2 吉林省长春市园林植物保护站
3.
3 国家林业局森林病虫害防治总站

基金项目:

东北林业大学学术名师支持计划项目(010602071)。

详细信息

作者简介:
张凯。主要研究方向:昆虫化学生态学。Email:1020654381@qq.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 严善春,教授,博士生导师。主要研究方向:昆虫化学生态学。Email:yanshanchun@126.com 地址:同上。

张凯。主要研究方向:昆虫化学生态学。Email:1020654381@qq.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 严善春,教授,博士生导师。主要研究方向:昆虫化学生态学。Email:yanshanchun@126.com 地址:同上。

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

1.
1 College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P. R. China;
2.
2 Changchun Landscape Plant Protection Station, Changchun, Jilin, 130022, P. R. China;
3.
3 General Station of Forest Pest Management, State Forestry Administration, Shenyang, Liaoning, 110034, P. R. China

摘要: 为了探讨重金属铜、镉污染胁迫下舞毒蛾体内排毒代谢酶活性的变化,本文以3种不同质量分数的铜(Cu,400、700、1 000 mg/kg)、镉(Cd, 1.0、3.0、6.0 mg/kg)处理土壤,用处理后的土壤盆栽1年生小黑杨扦插苗,研究舞毒蛾体内排毒代谢酶活性的变化趋势。结果发现,Cu、Cd不同浓度处理的杨树对舞毒蛾3龄、4龄幼虫体内排毒代谢酶活性有显著的干扰作用。与对照组相比,中、高质量分数Cu、Cd处理组3龄幼虫体内SOD、ACP和AKP酶活性显著降低(P0.05),低质量分数Cu、Cd处理组下降不显著(P0.05)。而POD和GSTs酶活性变化恰恰相反,中、高质量分数Cu、Cd处理组舞毒蛾3龄幼虫体内POD和GSTs酶活性显著升高(P0.05),低质量分数Cu、Cd处理升高不显著(P0.05)。中、高质量分数Cu、Cd处理下,4龄幼虫体内SOD、POD、ACP、AKP和GSTs酶活性和对照组相比显著降低(P0.05),低质量分数重金属处理和对照组相比下降不显著(P0.05)。以上结果显示,中、高质量分数Cu、Cd胁迫能显著干扰舞毒蛾幼虫的排毒代谢,且随着幼虫由3龄到4龄的发育,最终均表现为显著的抑制作用;低质量分数Cu、Cd胁迫对舞毒蛾幼虫排毒代谢干扰作用不显著。
- 重金属胁迫 /
- 小黑杨 /
- 舞毒蛾 /
- 排毒代谢酶
Abstract: In order to investigate the changes of detoxification metabolic activity in gypsy moth (Lymantria dispar) under the heavy metal pollution stress, we measured the detoxifying metabolism in gypsy moth body feeding on one-year potted seedlings of Populus simonii×P. nigra after treating the soil with three concentrations of copper (Cu, 400, 700, 1 000 mg/kg) and cadmium (Cd, 1.0, 3.0, 6.0 mg/kg). The results showed that Cu and Cd had a significant impact on the detoxification metabolic enzyme in the third and fourth instar larvae. The activities of SOD, ACP and AKP enzymes in vivo in the third instar larvae at medium and high concentrations of Cu and Cd were lower than the control group (P0.05),but at the low concentrations of Cu and Cd, the difference was not obvious (P0.05). The change of activities of POD and GSTs was just the opposite. The activities of POD and GSTs enzymes in the third instar larvae at medium and high concentrations of Cu and Cd increased significantly (P0.05), but not obvious (P0.05) at the low concentrations of Cu and Cd. The activities of SOD, POD, ACP, AKP and GSTs enzymes in the fourth instar larvae at medium and high concentrations of Cu and Cd decreased significantly (P0.05),but not obvious at the low concentrations of Cu and Cd treatment (P0.05). These results showed that the high levels of Cu and Cd stress could significantly interfere with the metabolic detoxification of gypsy moth larvae, and with the third instar larvae developing into the fourth instar, the heavy metal stress finally showed a significant inhibition to the development of larvae, while low levels of Cu and Cd stress had not significant interference with metabolic detoxification of the larvae.
- heavy metal stress /
- Populus simonii×P. nigra /
- Lymantria dispar /
- detoxifying metabolic enzyme

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铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

计量

铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

1. 1 东北林业大学林学院

2. 2 吉林省长春市园林植物保护站

3. 3 国家林业局森林病虫害防治总站

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Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

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铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

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铜、镉胁迫对舞毒蛾排毒代谢酶的影响

doi: 10.13332/j.1000-1522.20150293

1. 1 东北林业大学林学院 2. 2 吉林省长春市园林植物保护站 3. 3 国家林业局森林病虫害防治总站

English Abstract

Effects of copper and cadmium stress on the activities of detoxifying metabolic enzymes in Lymantria dispar

全文HTML

目录

1. 1 东北林业大学林学院

2. 2 吉林省长春市园林植物保护站

3. 3 国家林业局森林病虫害防治总站