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    覃责实, 石娟, 郝艳平, 崔骁芃, 李碧鹰. 基于氢稳定同位素技术对中国地区红脂大小蠹溯源追踪的可行性分析[J]. 北京林业大学学报, 2021, 43(5): 93-98. DOI: 10.12171/j.1000-1522.20190437
    引用本文: 覃责实, 石娟, 郝艳平, 崔骁芃, 李碧鹰. 基于氢稳定同位素技术对中国地区红脂大小蠹溯源追踪的可行性分析[J]. 北京林业大学学报, 2021, 43(5): 93-98. DOI: 10.12171/j.1000-1522.20190437
    Qin Zeshi, Shi Juan, Hao Yanping, Cui Xiaopeng, Li Biying. Feasibility analysis on species origin traceability by hydrogen stable isotope: sample case of Dendroctonus valens (Coleoptera: Scolytidae)[J]. Journal of Beijing Forestry University, 2021, 43(5): 93-98. DOI: 10.12171/j.1000-1522.20190437
    Citation: Qin Zeshi, Shi Juan, Hao Yanping, Cui Xiaopeng, Li Biying. Feasibility analysis on species origin traceability by hydrogen stable isotope: sample case of Dendroctonus valens (Coleoptera: Scolytidae)[J]. Journal of Beijing Forestry University, 2021, 43(5): 93-98. DOI: 10.12171/j.1000-1522.20190437

    基于氢稳定同位素技术对中国地区红脂大小蠹溯源追踪的可行性分析

    Feasibility analysis on species origin traceability by hydrogen stable isotope: sample case of Dendroctonus valens (Coleoptera: Scolytidae)

    • 摘要:
      红脂大小蠹是一种外来蛀干类害虫,它可以通过自然传播和人为传播途径进行远距离扩散。目前最为有效的防治手段就是及时进行监测和预警,但是由于缺乏快速追踪和溯源的手段,使得防治工作变得困难。
        目的  基于氢稳定同位素溯源技术,建立可用于追踪中国地区红脂大小蠹的溯源模型。
        方法  从全球降水氢同位素数据库中下载中国地区的栅格数据,利用ArcGIS 10.4.1构建中国大陆地区降水氢同位素区划图(坐标系统为Beijing 1954)。在5个样地累计诱捕红脂大小蠹成虫153头,利用稳定同位素质谱仪和元素分析仪测定样品的氢稳定同位素比值(δ2H)。利用R软件构建红脂大小蠹与降水δ2H值的标准曲线方程。
        结果  通过中国大陆地区降水氢同位素区划图得出:在东部季风区,降水δ2H值呈现出区域性变化,并大致表现为从东南向西北递减的梯度变化。5个样地的红脂大小蠹的δ2H值之间具有显著性差异。红脂大小蠹与降水的δ2H值呈正相关关系,并基于试验数据建立线性回归方程式:y = 3.166x + 86.050,经t检验、F检验、R2检验以及残差检验证明模型的显著性和匹配度都很高。
        结论  红脂大小蠹的δ2H值与其生境中的降水δ2H值呈现出高度相关的线性关系,且降水δ2H值在红脂大小蠹分布区具有可用于判别的规律。因此,基于本试验的数学模型可以实现中国地区红脂大小蠹的溯源追踪。

       

      Abstract:
      Dendroctonus valens (Coleoptera: Scolytidae) is an adventive trunk-boring pest, which can spread over long distances through natural and artificial transmission. Currently, the most effective means of prevention and control of this pest are timely monitoring and early warning. However, their implementation is usually hampered by the lack of feasible methods and tools for fast tracking and traceability.
        Objective  A traceability model that can be used to trace D. valens in China was established, based on hydrogen stable isotope technology.
        Method  The grid data of hydrogen stable isotopes of global precipitation were downloaded from the Online Isotopes in Precipitation Calculator (OIPC), and then, a zoning map of stable hydrogen isotopes of rainfall in mainland China was constructed using ArcGIS 10.4.1 (the coordinate system is Beijing 1954). The 153 D. valens adults captured in 5 regions were selected as experimental samples. The stable isotope mass spectrometer and elemental analyzer were used to measure the hydrogen stable isotope ratio (δ2H value) of the samples. Combined with the rainfall hydrogen isotope ratio of the sampling site, the data was simulated using R software.
        Result  From the zoning map of rainfall isotopes in the Chinese mainland, it can be clearly found that the δ2H isotope values of rainfall in the eastern monsoon region showed regional changes, and generally showed a gradient change from southeast to northwest. The δ2H stable isotope values of D. valens from the five geographical locations were significantly different. The δ2H stable isotope value of D. valens was positively correlated with the δ2H stable isotope value of rainfall. Based on the experimental data, a linear regression equation was established: y = 3.166x + 86.050. The t-test, F-test, R2 test and residual test proved that the model fit well.
        Conclusion  The hydrogen stable isotope characteristics of D. valens and the precipitation hydrogen stable isotope in its habitat present a highly correlated linear relationship, and the precipitation hydrogen stable isotope has regular changes that can be used for discrimination in the distribution area of D. valens. Therefore, based on the mathematical model of this experiment, the traceability of D. valens in China can be realized.

       

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