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    王雪怡, 顾咏梅, 张雪梅, 姜廷波, 刘焕臻. 杨树HSF家族基因生物信息学与胁迫应答表达分析[J]. 北京林业大学学报, 2021, 43(2): 34-45. DOI: 10.12171/j.1000-1522.20200159
    引用本文: 王雪怡, 顾咏梅, 张雪梅, 姜廷波, 刘焕臻. 杨树HSF家族基因生物信息学与胁迫应答表达分析[J]. 北京林业大学学报, 2021, 43(2): 34-45. DOI: 10.12171/j.1000-1522.20200159
    shu
    Wang Xueyi, Gu Yongmei, Zhang Xuemei, Jiang Tingbo, Liu Huanzhen. Bioinformatics and stress response expression analysis of poplar HSF family genes[J]. Journal of Beijing Forestry University, 2021, 43(2): 34-45. DOI: 10.12171/j.1000-1522.20200159
    Citation: Wang Xueyi, Gu Yongmei, Zhang Xuemei, Jiang Tingbo, Liu Huanzhen. Bioinformatics and stress response expression analysis of poplar HSF family genes[J]. Journal of Beijing Forestry University, 2021, 43(2): 34-45. DOI: 10.12171/j.1000-1522.20200159
    shu

    杨树HSF家族基因生物信息学与胁迫应答表达分析

    Bioinformatics and stress response expression analysis of poplar HSF family genes

      摘要
    • 摘要:
        目的  探究小黑杨热激转录因子HSF在应答高温和高盐胁迫时发挥的关键作用。保守结构域和顺式作用元件预测等对杨树HSF转录因子家族基因进行生物信息学分析。本研究以小黑杨为材料，经过37 ℃高温胁迫半个月后观察其形态变化；将小黑杨在37 ℃下分别处理0、12、24、48 h，采用RT-qPCR对小黑杨组织中的PsnHSFs基因进行时空表达分析；将小黑杨于150 mmol/L NaCl胁迫分别处理0、24 h，通过RNA-seq分析PsnHSFs基因的相对表达量变化，并通过RT-qPCR进行验证。
        结果  通过结构特征和系统发育比较将29个HSF转录因子家族基因分成A、B和C三个亚家族，各亚家族分别包含18、10和1个HSF基因；HSF编码的氨基酸序列长度介于209 ~ 595之间，均为亲水性蛋白；其N端具有高度保守的DBD结构域，由三个保守基序构成；HSF基因启动子序列中包含DRE core、ABRE和TC-rich等多种顺式作用元件。小黑杨经37 ℃高温处理后其株高仅为对照的76.51%，叶片呈卷曲状，叶表面粗糙，叶面积显著减小且苗干多侧枝柔软无韧性。RT-qPCR与RNA-seq结果表明，PsnHSFs被高温、高盐胁迫诱导表达。高温处理后其株高仅为对照的76.51%，叶片呈卷曲状，叶表面粗糙，叶面积显著减小且苗干多侧枝柔软无韧性。RT-qPCR与RNA-seq结果表明，PsnHSFs被高温、高盐胁迫诱导表达。家族基因以及揭示HSF参与木本植物胁迫应答的分子机制调控具有参考意义。

       

      Abstract:
        Objective  This paper aims to investigate the key role of heat shock transcription factor HSF of Populus simonii × P. nigra in response to high temperature and high salt stress.
        Method  Bioinformatics analysis of poplar HSF transcription factor family genes was carried out through multi-sequence alignment, phylogenetic tree construction, analysis of protein physicochemical properties, conserved domains and cis-acting element prediction. In this study, P. simonii × P. nigra was used as a material, and its morphological changes were observed after 37 ℃ high temperature treatment for half a month and the PsnHSFs genes were analyzed for spatio-temporal expression after 37 ℃ high temperature treatment for 0, 12, 24 and 48 h. In addition, P. simonii × P. nigra seedlings were used for 150 mM NaCl stress treatment for 0 and 24 h, the relative expression level of PsnHSFs was analyzed by RNA-seq and verified by RT-qPCR.
        Result  The 29 HSF genes were divided into three subfamilies of A, B and C by structural characteristics and phylogenetic comparison, each subfamily contained 18, 10 and 1 genes. The sequence length of amino acid HSF encoding was between 209 and 595. The HSF proteins were hydrophilic proteins; the N-terminal had a highly conserved DBD domain composed of three conserved motifs. The promoter sequences of the HSF genes contained a variety of cis-acting elements such as DRE core, ABRE and TC-rich elements. After high temperature treatment, the plant height was only 76.51% of control. The leaf was curled and rough, the leaf area was significantly reduced, and the trees had multiple branches which were soft and inflexible. RT-qPCR and RNA-seq results showed that PsnHSFs were induced by high temperature and high salt stress.
        Conclusion  The growth and development of poplar was significantly affected by high temperature, the PsnHSFs genes of poplar play an important role in response to high temperature and high salt stress. This study provides a reference for understanding the HSF family genes in poplar and revealing the molecular mechanism of HSF involved in stress response in woody plants.

       

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