Abstract: Regulators of G-protein signaling are important negative regulatory factors in G protein signaling pathway, which plays an important role in the realization of mycelial development, sporulation, secondary metabolites, pigment synthesis, pathogenicity and sexual reproduction regulation of fungi. In recent years, with the deepening of research on RGS of plant pathogenic filamentous fungi, a large number of academic reports have been produced. However, systematic comparative analysis of RGS in model fungi and phytopathogenic filamentous fungi has not been reported. In this study, the structure, classification and function of RGS of model fungi and plant pathogenic filamentous fungi were compared and analyzed. At the same time, through SMART conservative domain, secondary structure composition and genetic relationship analysis, RGS proteins in plant pathogenic filamentous fungi and model fungi were confirmed to have conservative RGS domain and similar secondary structure composition, also RGS proteins were divided into 6 categories according to sequence homology, and RGS proteins with different domains were clustered, respectively. The results showed that RGS proteins had certain conservativeness in functional performance in different fungi. In addition, the number and types of RGS in plant pathogenic filamentous fungi were found to be more than that of model organisms. The results show that RGS proteins have certain uniqueness in the functions in different fungi. The above research provides an important theoretical basis for further research on the mechanism of RGS in plant pathogenic filamentous fungi and the relationship between RGS in plant pathogenic filamentous fungi and other model organisms.