高级检索
    夏溪, 龚睿, 奉树成, 张春英. 映山红亚属内品种间杂交F1代花色苷种类及定量分析[J]. 北京林业大学学报, 2022, 44(5): 105-114. DOI: 10.12171/j.1000-1522.20210060
    引用本文: 夏溪, 龚睿, 奉树成, 张春英. 映山红亚属内品种间杂交F1代花色苷种类及定量分析[J]. 北京林业大学学报, 2022, 44(5): 105-114. DOI: 10.12171/j.1000-1522.20210060
    Xia Xi, Gong Rui, Feng Shucheng, Zhang Chunying. Types and quantitative analysis of anthocyanins in F1 hybrid among varieties in Rhododendron subgenus Tsutsusi[J]. Journal of Beijing Forestry University, 2022, 44(5): 105-114. DOI: 10.12171/j.1000-1522.20210060
    Citation: Xia Xi, Gong Rui, Feng Shucheng, Zhang Chunying. Types and quantitative analysis of anthocyanins in F1 hybrid among varieties in Rhododendron subgenus Tsutsusi[J]. Journal of Beijing Forestry University, 2022, 44(5): 105-114. DOI: 10.12171/j.1000-1522.20210060

    映山红亚属内品种间杂交F1代花色苷种类及定量分析

    Types and quantitative analysis of anthocyanins in F1 hybrid among varieties in Rhododendron subgenus Tsutsusi

    • 摘要:
        目的  花色是植物的重要观赏性状,花色苷在花色呈现中起着决定性作用。对花色苷种类及其含量进行分析,可以为解析花瓣呈色的机理奠定基础。
        方法  本研究以映山红亚属3个杂交组合的双亲及其子代共36份样品为对象,利用超高效液相色谱四极杆飞行时间质谱仪定性定量检测花瓣中花色苷的组分,并分析花色苷在亲子代中的分离变化规律。
        结果  本研究中共检测到17种化合物,其中芍药色素3-O-半乳糖苷、飞燕草素3-半乳糖苷、锦葵素3-O-半乳糖苷和矮牵牛素3-半乳糖苷首次在映山红亚属植物中检测到。映山红亚属植物中的花青素以矢车菊素和芍药色素为主,且主要以阿拉伯糖苷的形式存在。其中矢车菊素3-阿拉伯糖苷、矢车菊素3-O-葡萄糖苷和芍药色素与映山红亚属花瓣的红色着色相关。飞燕草素3-阿拉伯糖苷、飞燕草素3-O-葡萄糖苷、锦葵素3-阿拉伯糖苷和锦葵素3-O-葡萄糖苷与映山红亚属花瓣的紫色着色相关,矮牵牛素3-O-阿拉伯糖苷与花瓣紫红色着色相关。花色苷的分离分析结果表明,矢车菊素类和芍药色素类糖苷表现出超亲遗传的特性,锦葵素3-阿拉伯糖苷、锦葵素3-O-葡萄糖苷、矮牵牛素3-O-阿拉伯糖苷表现出偏向父本遗传的特性。
        结论  本研究从花色苷种类和含量角度解析了映山红亚属花色呈色机制,同时也为花色育种的亲本选择提供了参考资料。

       

      Abstract:
        Objective  Color is the major ornamental characteristics of plants, anthocyanins are the key compounds of flower colors. The types and contents of anthocyanins can provide insight into the mechanism of petal color.
        Method  In this study, 36 samples of three pairs of Rhododendron subgenus Tsutsusi parents and their hybrids were subjected to anthocyanins profiling in petals and their separation characteristics using UPLC –Q –TOF –MS.
        Result  This study identified 17 compounds, including peonindin 3-O-galactoside, delphinidin 3-galactoside, malvidin 3-O-galactoside, and petunidin 3-galactoside, which were detected for the first time in Rhododendron subgenus Tsutsusi. A majority of anthocyanins in Rhododendron subgenus Tsutsusi are cyanidin, peonidin, and petunidin in the form of arabinosides. Cyanidin 3-arabinoside, cyanidin 3-O-glucoside and peonidin are responsible for the red-colored petals in Rhododendron subgenus Tsutsusi. Delphinidin 3-arabinoside, delphinidin 3-O-glucoside, malvidin 3-arabinoside, and malvidin 3-O-glucoside contribute to the petal’s purple color, while petunidin 3-O-arabinoside contributes to their purplish red color. The anthocyanin segregation analysis revealed that peonidin and cyanidin had transgressive inheritance, whereas malvidin 3-arabinoside, malvidin 3-O-glucoside, and petunidin 3-O-arabinoside had paternal inheritance.
        Conclusion  In this study, we demonstrate the coloring mechanism of Rhododendron subgenus Tsutsusi, which provides a reference for parent selection in breeding.

       

    /

    返回文章
    返回