高级检索
    牛春阳, 王峰, 李丹蕾, 陈俏丽, 张瑞芝. C14 族R2R3鄄MYB 基因调控杨树抗锈菌过敏性反应[J]. 北京林业大学学报, 2016, 38(7): 25-32. DOI: 10.13332/j.1000-1522.20150499
    引用本文: 牛春阳, 王峰, 李丹蕾, 陈俏丽, 张瑞芝. C14 族R2R3鄄MYB 基因调控杨树抗锈菌过敏性反应[J]. 北京林业大学学报, 2016, 38(7): 25-32. DOI: 10.13332/j.1000-1522.20150499
    NIU Chun-yang, WANG Feng, LI Dan-lei, CHEN Qiao-li, ZHANG Rui-zhi.. Impact of clade 14 R2R3-MYB genes on hypersensitive response of the poplar infected with Melampsora larici-populina.[J]. Journal of Beijing Forestry University, 2016, 38(7): 25-32. DOI: 10.13332/j.1000-1522.20150499
    Citation: NIU Chun-yang, WANG Feng, LI Dan-lei, CHEN Qiao-li, ZHANG Rui-zhi.. Impact of clade 14 R2R3-MYB genes on hypersensitive response of the poplar infected with Melampsora larici-populina.[J]. Journal of Beijing Forestry University, 2016, 38(7): 25-32. DOI: 10.13332/j.1000-1522.20150499

    C14 族R2R3鄄MYB 基因调控杨树抗锈菌过敏性反应

    Impact of clade 14 R2R3-MYB genes on hypersensitive response of the poplar infected with Melampsora larici-populina.

    • 摘要: 由落叶松-杨栅锈菌引起的杨树叶锈病分布广泛,危害严重。为探究杨树C14族R2R3-MYB基因在杨树抗锈菌过敏性反应中的调控作用,对接种了落叶松-杨栅锈菌E4强致病性生理小种的2种杨树叶片进行症状观察、感病指数判定并对2种杨树C14族共6条R2R3-MYB基因表达量变化情况进行分析。接种7 dpi后,弱感病性树种杂交杨叶片产生的夏孢子堆数量显著少于感病树种欧美杨。杂交杨叶片4 dpi开始出现过敏性反应症状,欧美杨未观察到类似症状。表明过敏性反应可有效阻止锈菌夏孢子堆形成,降低杨树感病性。杂交杨与欧美杨R2R3-MYB基因表达量变化趋势相反,预测杨树C14族R2R3-MYB基因为杨树与病原互作过程中调控过敏性反应的正向调控因子。本研究为进一步系统研究杨树R2R3-MYB基因与过敏性反应的调控机理及杨树抗病育种工作提供理论依据。

       

      Abstract: Leaf rust caused by Melampsora larici-populina is the main disease affecting poplar plantations, causing severe economic losses. To explore the regulatory effects of clade 14 R2R3-MYB genes on hypersensitive response of the poplar infected with M. larici-populina, leaves of two poplar species were inoculated by M. larici-populina E4 pathotypes to observe the symptom and score the infectious index. In addition, changes of the gene expression level of six clade 14 R2R3-MYB genes were analyzed. After seven days of inoculation, the number of uredinium on Populus deltoidesP. trichocarpa (less susceptible to M. larici-populina E4 pathotypes) leaves was significantly less than that on P.euramericana (very susceptible to M. larici-populina E4 pathotypes). On the fourth day of inoculation, symptoms of hypersensitive reaction (HR) were observed on leaves of P. deltoidesP. trichocarpa, but not on Populuseuramericana leaves. It turns out that HR can prevent the formation of uredinium and reduce the susceptibility of poplar. The change trend of R2R3-MYB gene expression level of the two poplar species was contrary. It is estimated that poplar clade 14 R2R3-MYB genes may be positive regulators of the HR in host in response to rust attack. This study provides a theoretical foundation for further studying these transcriptional factors in the control of the HR and the possible usage of these genes for the production of rust-resistant poplar.

       

    /

    返回文章
    返回