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    苗禹博, 朱晓梅, 李志娟, 贾凤岭, 李伟. 不同世代樟子松育种资源遗传评价[J]. 北京林业大学学报, 2017, 39(12): 71-78. DOI: 10.13332/j.1000-1522.20170194
    引用本文: 苗禹博, 朱晓梅, 李志娟, 贾凤岭, 李伟. 不同世代樟子松育种资源遗传评价[J]. 北京林业大学学报, 2017, 39(12): 71-78. DOI: 10.13332/j.1000-1522.20170194
    MIAO Yu-bo, ZHU Xiao-mei, LI Zhi-juan, JIA Feng-ling, LI Wei. Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations[J]. Journal of Beijing Forestry University, 2017, 39(12): 71-78. DOI: 10.13332/j.1000-1522.20170194
    Citation: MIAO Yu-bo, ZHU Xiao-mei, LI Zhi-juan, JIA Feng-ling, LI Wei. Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations[J]. Journal of Beijing Forestry University, 2017, 39(12): 71-78. DOI: 10.13332/j.1000-1522.20170194

    不同世代樟子松育种资源遗传评价

    Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations

    • 摘要: 当前樟子松遗传改良正处于升级换代的关键时期,但是不同地区育种资源调拨与交换频繁,导致了育种资源信息模糊、亲缘关系混乱,增加了樟子松遗传改良工作中近交衰退的风险,因此开展育种资源评价研究是樟子松优良单株利用的重要基础。本文以樟子松1、1.5、2代种子园224个无性系为研究对象,开展了基于SSR的遗传多样性和单株亲缘关系分析以及遗传差异分析。结果表明:按育种资源的来源分析,良种基地、头道桥、榆林1.5和泰来4个群体的遗传多样性水平较高,宝根园和榆林群体相对较低;按育种资源的世代分析,不同世代樟子松育种资源维持着较高的遗传多样性水平,高世代种子园的遗传多样性水平有所下降,2、1.5代种子园的期望杂合度He较1代种子园分别下降4.13%、1.31%。2代种子园群体的近交系数F值(0.173)较高,纯合子较多,显著偏离了哈迪-温伯格平衡。同种源、不同生境的樟子松育种资源没有发生明显的变异,主要的遗传变异存在于个体之间(96%);随着种子园世代的提高,无性系之间的亲缘关系聚类现象逐渐明显,2代种子园内存在两组完全一致的无性系(CH3、CH10组和TL1417、TL1411组)。研究结果完善了樟子松育种资源评价体系,确定了不同世代育种资源的亲缘关系,为樟子松高级遗传改良工作提供了材料和信息基础。

       

      Abstract: Nowadays, the genetic improvement of Pinus sylvestris var. mongolica is in a critical period of upgrading and updating. But the frequent allocation and exchange of breeding resources in different areas lead to unclear information of breeding resources and confusion of genetic relationship, which increases the risk of inbreeding depression during genetic improvement of Pinus sylvestris var. mongolica. Therefore, the evaluation of breeding resources is an important basis for the utilization of superior individuals of Pinus sylvestris var. mongolica. In this paper, 224 clones of Pinus sylvestris var. mongolica from the 1st, 1.5th and 2nd seed orchards were studied. Genetic diversity, phylogenetic relationship and genetic diversity were analyzed based on SSR, and the population of BaogenYuan and Yulin was relatively low according to breeding resource analysis; the genetic diversity of four populations of LiangZhongJiDi, TouDaoQiao, YuLin1.5 and TaiLai was higher.According to improved generation analysis, different generations of Pinus sylvestris var. mongolica breeding resources maintained a high level of genetic diversity; the genetic diversity of the high generation seed orchard decreased, and the expected heterozygosity (He) of the 2nd and 1.5th generation seed orchards decreased by 4.13% and 1.31% respectively compared with the 1st generation seed orchard; the 2nd generation seed orchard group had more higher inbreeding coefficient (the F-value was 0.173) and homozygote, which significantly deviated from Hardy-Weinberg equilibrium; Pinus sylvestris var. mongolica breeding resources in different habitats and the same source had no obvious variation, the genetic variation mainly existed among individuals (96%); with the generation seed orchard increased, phylogenetic relationship clustering phenomenon among clones gradually became obvious, two sets of breeding resource clones were identified completely genetic consistent in the 2nd generation seed orchard. The results of this research improve the breeding resource evaluation system of Pinus sylvestris var. mongolica, determine the genetic relationship of different generation breeding resources, and provide the material and information basis for the advanced genetic improvement work of Pinus sylvestris var. mongolica.

       

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