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    刘露露, 鲁婷婷, 王爽, 李美靓, 赵双菁, 刘莹莹, 魏志刚. 杨树PsnGA20ox1过表达对烟草叶片发育的影响[J]. 北京林业大学学报, 2018, 40(2): 22-30. DOI: 10.13332/j.1000-1522.20170245
    引用本文: 刘露露, 鲁婷婷, 王爽, 李美靓, 赵双菁, 刘莹莹, 魏志刚. 杨树PsnGA20ox1过表达对烟草叶片发育的影响[J]. 北京林业大学学报, 2018, 40(2): 22-30. DOI: 10.13332/j.1000-1522.20170245
    Liu Lu-lu, Lu Ting-ting, Wang Shuang, Li Mei-liang, Zhao Shuang-jing, Liu Ying-ying, Wei Zhi-gang. Effects of poplar PsnGA20ox1 overexpression on leaf development of tobacco[J]. Journal of Beijing Forestry University, 2018, 40(2): 22-30. DOI: 10.13332/j.1000-1522.20170245
    Citation: Liu Lu-lu, Lu Ting-ting, Wang Shuang, Li Mei-liang, Zhao Shuang-jing, Liu Ying-ying, Wei Zhi-gang. Effects of poplar PsnGA20ox1 overexpression on leaf development of tobacco[J]. Journal of Beijing Forestry University, 2018, 40(2): 22-30. DOI: 10.13332/j.1000-1522.20170245

    杨树PsnGA20ox1过表达对烟草叶片发育的影响

    Effects of poplar PsnGA20ox1 overexpression on leaf development of tobacco

    • 摘要:
      目的赤霉素(Gibberellins, GA)参与植物多个生长发育过程,然而赤霉素在植物叶片发育中的生物学功能尚无研究报道。GA20ox是GAs生物合成途径中关键限速酶(GA20-oxidases)的编码基因,本项研究目的是揭示PsnGA20ox1在植物叶片发育中的生物学功能。
      方法本项研究分析了PsnGA20ox1在烟草中过表达后对烟草叶片大小、超微结构、生物量、叶绿素及生物活性GAs含量,以及细胞分裂与气孔发育相关的基因表达的影响。
      结果PsnGA20ox1过表达烟草叶片变大、叶绿素与GA4含量上升,叶片表皮细胞变小。而且,转基因烟草叶片栅栏组织与海绵组织厚度、表皮细胞与气孔数目、光合作用和叶片生物量与野生型烟草相比均显著增加。此外,转基因烟草叶片气孔与表皮细胞发育相关基因表达也发生了显著变化,而且变化趋势与叶片表型的改变相吻合。
      结论PsnGA20ox1过表达影响植物叶片的结构与功能,从而提高了叶片的生物量。

       

      Abstract:
      ObjectiveGA20ox is one of the genes coding key rate-limiting enzymes, GA20-oxidases (GA20ox), for biosynthesis of bioactive gibberellins (GA) which involve in many biology process of plant growth and development. At present, it is still not clear that the biological function of GAs in plant leaf development. The aim of this research is to reveal the roles of PsnGA20ox1 in the leaves of overexpression tobacco.
      MethodIn this study, we systematically analyzed the leaf size, ultrastructure, biomass, chlorophyll and bioactivity GAs contents, and gene expression level involved in cell division and stomatal formation of PsnGA20ox1 overexpression tobacco and wild-type plants.
      ResultOur results revealed that the PsnGA20ox1 overexpression tobacco displayed the enlarged leaf size, enhanced chlorophyll and GA4 contents, and decreased epidermal cell size. In addition, the thickness of palisade and spongy parenchyma, the sizes of epidermal cell, the numbers of stomatal and epidermal cell, and photosynthesis and leaf biomass productivity of PsnGA20ox1 overexpression tobacco were notably increased compared with wild-type tobacco, respectively. Furthermore, the expression levels of genes involved in cell division and stomatal formation in PsnGA20ox1 overexpression tobacco altered notably, and the variation tendency was in accordance with the alternations of leaf trait.
      ConclusionThe results demonstrated that the overexpression of PsnGA20ox1 had effect on the structure and development of leaf in the transgenic tobacco, which is beneficial to the accumulation of biomass of leaf.

       

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