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    姜礅, 王月月, 严善春. 锌胁迫对银中杨生长发育和化学防御的影响[J]. 北京林业大学学报, 2018, 40(11): 42-48. DOI: 10.13332/j.1000-1522.20180131
    引用本文: 姜礅, 王月月, 严善春. 锌胁迫对银中杨生长发育和化学防御的影响[J]. 北京林业大学学报, 2018, 40(11): 42-48. DOI: 10.13332/j.1000-1522.20180131
    Jiang Dun, Wang Yueyue, Yan Shanchun. Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings[J]. Journal of Beijing Forestry University, 2018, 40(11): 42-48. DOI: 10.13332/j.1000-1522.20180131
    Citation: Jiang Dun, Wang Yueyue, Yan Shanchun. Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings[J]. Journal of Beijing Forestry University, 2018, 40(11): 42-48. DOI: 10.13332/j.1000-1522.20180131

    锌胁迫对银中杨生长发育和化学防御的影响

    Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings

    • 摘要:
      目的探明Zn胁迫对银中杨生长发育的影响及银中杨化学防御对Zn胁迫的响应趋势。
      方法本研究用3种不同含量的Zn(300、500、700 mg/kg)处理盆栽1年生银中杨的土壤,分析其生长发育状况、叶片内营养物质和次生物质含量以及蛋白酶抑制剂活性。
      结果Zn各含量胁迫使银中杨的生长参数(株高、根长和地径)和生物量参数(根、茎、叶的鲜质量和干质量)均显著低于对照(P<0.05),且具有一定的浓度效应。在Zn胁迫后30、40和50 d,各含量处理均使银中杨叶片中的蛋白质和可溶性糖含量显著低于对照(P<0.05),但处理组之间的蛋白质含量差异均不显著(P>0.05),而可溶性糖含量均表现为随Zn胁迫含量的增高而增高。Zn各含量胁迫均使银中杨叶片内的黄酮和木质素含量在各时间段显著高于对照,而叶片内的胰蛋白酶抑制剂(TI)和胰凝乳蛋白酶抑制剂(CI)活性却均显著低于对照(P<0.05)。
      结论生长基质中过量的Zn会抑制银中杨的生长发育,且能影响银中杨的化学防御水平。银中杨叶片内营养物质含量、抗虫相关次生代谢物质的含量、以及蛋白酶抑制剂活性对Zn胁迫的响应趋势不一致,体现为其营养物质含量减少、次生物质含量增多、蛋白酶抑制剂活性减弱。

       

      Abstract:
      ObjectiveThis study aims to investigate the effects of Zn stress on growth development of Populus alba 'berolinensis' (PAB) seedlings and their chemical defense patterns in response to the Zn stress.
      MethodWe analyzed the growth development status, nutrient content and secondary metabolites, as well as the protease inhibitor activities in the leaves of PAB seedlings growing in either non-contaminated soil (CK/control) or Zn-contaminated soil (300, 500 and 700 mg/kg).
      ResultOur results showed that the growth parameters (plant height, root length and ground diameter) and biomass parameters (the fresh and dry mass of root, stem and leaf) of the PAB seedlings in all the Zn-treated groups were significantly lower than those in the control (P < 0.05) in a concentration/dose-dependent fashion. On the 30th, 40th and 50th days after Zn stress, the contents of proteins and soluble sugars in PAB leaves in all the Zn-treated groups were significantly lower than those in control (P < 0.05). There were no significant differences in protein contents between the Zn treatment groups (P>0.05), while soluble sugar contents increased with the increase of Zn concentration. Zn stress, regardless of its concentration level, significantly increased the contents of flavonoids and lignin in the leaves on all three sampling dates compared with the control, whereas the trypsin inhibitor (TI) and the chymotrypsin inhibitor (CI) activities were significantly decreased (P < 0.05).
      ConclusionThe above results illustrate that excessive Zn in the growing environment may inhibit the growth of PAB seedlings and affect their chemical defense. The response patterns of nutrient substances, secondary metabolites related to the plant resistance to insects as well as the protease inhibitors in the PAB leaves to Zn stress are inconsistent, as was evidenced by the reduced nutrient contents, the increased secondary metabolite contents and the weakened protease inhibitor activities.

       

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