高级检索
    李介文, 杜运鹏, 贾桂霞, 张冬梅. 我国部分百合野生资源的亲缘关系及其分布特点[J]. 北京林业大学学报, 2019, 41(10): 74-82. DOI: 10.13332/j.1000-1522.20180345
    引用本文: 李介文, 杜运鹏, 贾桂霞, 张冬梅. 我国部分百合野生资源的亲缘关系及其分布特点[J]. 北京林业大学学报, 2019, 41(10): 74-82. DOI: 10.13332/j.1000-1522.20180345
    Li Jiewen, Du Yunpeng, Jia Guixia, Zhang Dongmei. Genetic relationship analysis and distribution characteristics of some wild Lilium species native to China[J]. Journal of Beijing Forestry University, 2019, 41(10): 74-82. DOI: 10.13332/j.1000-1522.20180345
    Citation: Li Jiewen, Du Yunpeng, Jia Guixia, Zhang Dongmei. Genetic relationship analysis and distribution characteristics of some wild Lilium species native to China[J]. Journal of Beijing Forestry University, 2019, 41(10): 74-82. DOI: 10.13332/j.1000-1522.20180345

    我国部分百合野生资源的亲缘关系及其分布特点

    Genetic relationship analysis and distribution characteristics of some wild Lilium species native to China

    • 摘要:
      目的分析我国部分野生百合资源的遗传相似性及其地域分布特点,对其亲缘关系进行评价,为百合分子标记辅助育种提供依据。
      方法以25个百合野生种及其不同种源地的2个变种,共计69份样品为材料,利用11对SSR引物,对69份样品进行荧光毛细管电泳检测,通过Powermarker 3.25软件对SSR位点基因型数据进行处理,通过NTsys 2.11构建遗传相似性矩阵及系统发育树,对其进行聚类分析,并进行主坐标分析。
      结果对SSR分子标记的遗传学参数进行分析,发现百合SSR位点多态性较高。69份样品的遗传相似性系数变化范围为0.289 7 ~ 0.981 3:不同种之间,淡黄花百合和泸定百合的遗传相似性系数最大,平均值是0.953 3;而卷丹和玫红百合、卷丹和大理百合之间的相似性系数最小,平均值是0.289 7。聚类分析表明,69份供试材料主要分为5类,第I类包括大百合属的大百合,第II类主要由卷瓣组和喇叭组组成,同时也包括轮叶组和钟花组,第III、IV、V类主要由卷瓣组组成,其中包括豹子花属的滇蜀豹子花。主坐标分析表明种源地位于四川、重庆、云南地区的百合大多聚在一起,位于陕西、湖北、湖南、河南地区的百合大多聚在一起,位于辽宁、内蒙古地区的百合大多聚在一起。
      结论本研究表明喇叭组a亚组与b亚组亲缘关系较远,a亚组的麝香百合、台湾百合明显与卷瓣组亲缘关系较近;属外种大百合与百合属亲缘关系较远,滇蜀豹子花与野生百合资源的亲缘关系较近,因此本研究支持豹子花属并入百合属这一观点;从地域上证明,不同种源地的同种百合随地域差异变大导致遗传相似系数降低,进而导致其在进化上产生分歧,不同种野生百合空间地理分布较近的因生境相似而对环境有相同的反应,有趋同进化的趋势。

       

      Abstract:
      ObjectiveThe objective of this study is to analyze the genetic similarity, genetic relationship and geographical distribution characteristics of some wild Lilium species native to China. And this study provides useful information for molecularly assisted breeding of Lilium spp.
      Method11 core SSR primers were used to scan 69 accessions of 25 wild species and 2 varieties in Lilium spp. by capillary electrophoresis platform and 69 samples were evaluated via the software Powermarker ver.3.25. The genetic similarity matrix and phylogenetic tree were constructed by NTsys 2.11 for cluster analysis. Principal coordinate analysis was performed using NTSYS-2.11.
      ResultBased on the analysis of genetic parameters of SSR markers, the results showed that the SSR loci were polymorphism. The genetic similarity coefficient of 69 samples varied from 0.289 7 to 0.981 3. Among the different species, the genetic similarity coefficient of L. sulphureum and L. sargentiae was the largest, with an average of 0.953 3; while the genetic similarity coefficient of L. lancifolium and L. amoenum, L. lancifolium and L. taliense was the smallest, with an average of 0.289 7. Cluster analysis showed that 69 materials were divided into 5 groups, Group I consisted of the Cardiocrinum giganteum. The second group mainly consisted of the sect. Sinomartagon and the sect. Leucolirion, as well as the sect. Martagon and the sect. Lophophorum. Group III, IV and V are mainly composed of the sect. Sinmartagon, and including the Nomocharis forrestii. The main coordinate analysis showed that the Lilium species in Sichuan, Chongqing and Yunnan regions mostly gathered together. The Lilium species in Shanxi, Hubei, Hunan and Henan provinces mostly gathered together. The Lilium species in Liaoning Province and Inner Mongolia Autonomous Region mostly gathered together.
      ConclusionThis study shows that subsect. Leucolirion a is obviously far away from subsect. Leucolirion b, but L. longiflorum and L. formosanum from subsect. Leucolirion a are closely related with sect. Sinomartagon. Besides, the Cardiocrinum giganteum is obviously far away from the Lilium spp. The relationship between Nomocharis forrestii and Liliun spp. is close, supporting the view that the Nomocharis is classified into the Lilium. At the same time, this study proves that with the increase of regional difference, the genetic similarity coefficient between Lilium in different provenances was decreased, which leading to their evolutionary differences. Different species of Llilium in the same provenance have the same spatial response to the environment, and they may have a trend towards convergence evolution.

       

    /

    返回文章
    返回