Abstract: A study for determining the decolorizing capacity of white rot basidiomycete Cerena unicolor strain CB1 was carried out. Based on cloning of ITS sequence, factors affecting decolorization into two dyes, reactive black and reactive red, were carried out. The results of phylogenetic analysis showed that the ITS sequence of C. unicolor CB1 was 93% to 99% homologous to 17 different C. unicolor strains, indicating that strain CB1 was C. unicolor. The results of decolorization tests showed that the reactive black dye inhibited the decolorization reaction of C. unicolor CB1 evidently at the concentration of 250 mg/ L, whereas reactive red had no significant inhibition to the decolorization reaction even at the concentration of 500 mg/ L. The most optimal carbon source for C. unicolor CB1 to decolor reactive black and reactive red was fructose and glucose, respectively; The best nitrogen source was urea and ammonium nitrate, respectively. The optimal concentration of Cu2+ and Mn2+ both was 0.1 mmol/ L, the optimum pH was 5, and the optimum inoculum was seven fresh mycelium discs with d =8 mm.