Abstract: In order to avoid the great resource waste and sufficiently utilize the Larix gmelinii bark, supercritical carbon dioxide extraction was used to extract the liposoluble substance from L. gmelinii bark. The liposoluble substance was thoroughly extracted from 3.00 kg bark in 150 min and the yield of extracts was 3.325%. The extracts were examined by GC-MS, and 44 components were identified. The terpenes and their oxo-derivatives were major chemicals in the extracts. The relative content of components was determined with peak area normalization method, the major constituents of the extracts were 5, 6-ol-Carotene(36.30%), resin acids and their derivatives (27.60%), manool (17.14%), geranyl linalool (12.51%), all of these constituents were of high economic value. They are either bioactive substances, or the important chemical intermediates of fine chemicals and spice chemical. The SFE-CO2 extracts from L. gmelinii bark are different from the normal resin, which are worth further research and are considered with great prospects.