高级检索
    陶翠, 王捷, 姚玉军, 韩雪, 吕兆林. 沙棘中白雀木醇表征方法及其分布规律[J]. 北京林业大学学报, 2020, 42(1): 121-126. DOI: 10.12171/j.1000-1522.20190200
    引用本文: 陶翠, 王捷, 姚玉军, 韩雪, 吕兆林. 沙棘中白雀木醇表征方法及其分布规律[J]. 北京林业大学学报, 2020, 42(1): 121-126. DOI: 10.12171/j.1000-1522.20190200
    Tao Cui, Wang Jie, Yao Yujun, Han Xue, Lü Zhaolin. Characterization and distribution rule of quebrachitol in Hippophae rhamnoides[J]. Journal of Beijing Forestry University, 2020, 42(1): 121-126. DOI: 10.12171/j.1000-1522.20190200
    Citation: Tao Cui, Wang Jie, Yao Yujun, Han Xue, Lü Zhaolin. Characterization and distribution rule of quebrachitol in Hippophae rhamnoides[J]. Journal of Beijing Forestry University, 2020, 42(1): 121-126. DOI: 10.12171/j.1000-1522.20190200

    沙棘中白雀木醇表征方法及其分布规律

    Characterization and distribution rule of quebrachitol in Hippophae rhamnoides

    • 摘要:
      目的本论文基于一种高效、准确白雀木醇表征方法,对沙棘资源中的白雀木醇进行准确定量,探究沙棘资源中白雀木醇的含量变化规律,为沙棘中白雀木醇的开发利用提供参考。
      方法对比了气相色谱法(GC)和高效液相色谱法(HPLC)两种测试手段的优缺点,采用较优的液相色谱测试方法对包括沙棘果、沙棘籽、沙棘叶在内的沙棘资源中白雀木醇含量变化进行了研究。
      结果采用液相色谱−蒸发光散射法测定沙棘资源中的白雀木醇,该法呈现良好的线性关系(R2 = 0.998 5),且精密度高(RSD ≤ 0.53%)、准确度高(RSD ≤ 1.16%)、重复性好(RSD ≤ 1.71%)。研究结果显示,沙棘中不同部位的白雀木醇含量差异显著(P < 0.01),其中沙棘叶中白雀木醇含量最高(59.73 mg/g,干叶),其次为沙棘果肉(2.19 mg/g,鲜果肉)和沙棘籽(0.39 mg/g,鲜籽);收集5月份至9月份不同生长阶段的沙棘叶,研究表明,随着叶子生长白雀木醇含量呈现为逐渐升高趋势,且7月份至8月份期间增速最大,9月份含量积累至最高(64.79 mg/g,干叶)。
      结论液相色谱−蒸发光散射法检测白雀木醇含量,操作简便、并且精密度、准确度高、重复性好;沙棘叶中白雀木醇含量较果实丰富,沙棘叶中白雀木醇含量9月份累积至最高,本研究可为沙棘资源中白雀木醇的开发应用提供有效数据支撑。

       

      Abstract:
      ObjectiveBased on an efficient and accurate characterization method, we accurately quantified quebrachitol and explored the changing rules of its content in seabuckthorn, providing references for the development and utilization of seabuckthorn resources.
      MethodAfter the comparison of the two methods, HPLC was selected for the determination of content of quebrachitol in three different parts of seabuckthorn, including the flesh, seed and leaf.
      ResultThis method showed a good linear relationship (R2 = 0.998 5), with high precision (RSD ≤ 0.53%), high accuracy (RSD ≤ 1.16%) and good repeatability (RSD ≤ 1.71%). The results showed that there was a significant difference in the content of alcohol in different parts of seabuckthorn (P < 0.01), and the content in the leaves of seabuckthorn was the highest (59.73 mg/g dry leaves), followed by the flesh of seabuckthorn (2.19 mg/g fresh flesh) and the seeds of seabuckthorn (0.39 mg/g fresh seeds). The leaves of seabuckthorn at different growth stages from May to September were collected, and the results showed that the content of quebrachitol increased gradually with the growth of leaves, and the growth rate was the largest from July to August, and the content accumulated to the highest in September (64.79 mg/g dry leaves).
      ConclusionLiquid chromatography-evaporative light scattering detector was found easy operating, accurate, and repeatable for the determination of quebrachitol content. The quebrachitol content in the leaves of seabuckthorn was more abundant than that in the fruits, and it reached the highest in September. This study can provide effective data support for the development and application of quebrachitol in seabuckthorn resources.

       

    /

    返回文章
    返回