Abstract: Species diversity of macrofungi at Wangyedian Forest Farm was systematically investigated in 2012. More than 350 specimens were collected from pine forest, larch forest, natural mixed forests, and natural-artificial mixed forest at the regions including Duanmugou, Xinkaiba, Liudaogou, Meilin, and Maojingba, etc. Diversity analysis revealed that the wild macrofungi were abounding at the Wangyedian Forest Farm. Total 162 species were found, which belong to 2 phyla, 4 classes, 12 orders, 41 families, and 79 genera. The diversity and abundance of macrofungi were different in these types of forests, most of which was natural mixed forests, followed by larch forest, pine forest, and natural-artificial mixed forest. The dominant families of these macrofungi were Agaricaceae, Russulaceae, and Polyporaceae. Twelve species are new to Inner Mongolia and 2 species are new records in China. Polyporus umbellatus (Pers. ) Fr. , a rarely medicinal wild macrofungi, was also collected in this area. The directory of wild macrofungi was provided and this will lay a good foundation for their protection and application in the future.