Abstract: The concentration of negative air ions in different seasons was monitored in typical garden areas of Beijing, i.e. the Fragrant Hill, the Summer Palace and the Botanical Garden. Comparative analyses were conducted combined with the results from open spaces and urban areas. The results show that the presence of landscape plants can significantly enhance the negative air ion concentration, which was significantly higher in vegetation areas than that in nonvegetation areas. The negative air ion concentration showed a single peak curve with increasing elevation and was closely correlated with the condition of the vegetation at specific elevations. The diurnal fluctuation of negative air ion concentration in garden areas showed a bimodal curve. The negative air ion concentration in different habitats changed clearly with the seasons, with higher concentrations in the summer and autumn. 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