Abstract: High biomass and leaf nutritive value of Robinia pseudoacacia can both be got from a feasible cultivation model, which can make the best use of forage forest. Therefore, a study was conducted to assess the impact of three cultivation models on biomass and leaf nutrition in tetraploid R. pseudoacacia clone K5, compare the biomass among three clones of tetraploid R. pseudoacacia and one diploid R. pseudoacacia under the cultivation model of harvesting one time per year, and compare the biomass among three clones of tetraploid R. pseudoacacia under the cultivation model of harvesting two times per year. Results indicated that crude protein in leaves from the second harvest of harvesting two times per year was significantly higher (P0.05) than that from the other two cultivation models, while there were no significant differences in leaf weight per plant among these three cultivation models. Gross weight and leaf weight per plant of tetraploid R. pseudoacacia clone K2 were significant higher than those of tetraploid R. pseudoacacia clone K4, K5 and diploid R. pseudoacacia under the cultivation model of harvesting one time per year, and they also were higher than those of tetraploid R. pseudoacacia clone K4 under the cultivation model of harvesting two times per year. Considering leaf biomass and leaf nutrition, tetraploid R. pseudoacacia clone K2 was better than tetraploid R. pseudoacacia clone K4, K5 and diploid R. pseudoacacia for forage forest, and its suitable cultivation model should be harvesting from second year after planting and harvesting two times per year (harvesting in early June and late August while no cutting in spring).