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    马庆国, 王滑, 潘刚, 裴东. 西藏核桃优良单株的FISH-AFLP分析[J]. 北京林业大学学报, 2011, 33(6): 86-89.
    引用本文: 马庆国, 王滑, 潘刚, 裴东. 西藏核桃优良单株的FISH-AFLP分析[J]. 北京林业大学学报, 2011, 33(6): 86-89.
    shu
    MA Qing-guo, WANG Hua, PAN Gang, PEI Dong. FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.[J]. Journal of Beijing Forestry University, 2011, 33(6): 86-89.
    Citation: MA Qing-guo, WANG Hua, PAN Gang, PEI Dong. FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.[J]. Journal of Beijing Forestry University, 2011, 33(6): 86-89.
    shu

    西藏核桃优良单株的FISH-AFLP分析

    FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.

      摘要
    • 摘要: 为了深入了解西藏核桃种质资源的遗传背景和遗传多样性，采用FISHAFLP技术，筛选9 对EcoRⅠ+3和MseⅠ+3引物组合，对我国西藏核桃主产区加查和郎县的11个核桃(Juglans regia L.)优良单株进行基因组DNA水平检测。结果表明：在获得的1 103条可统计条带中，1 076 条呈多态性，多态性达97.67%；9对引物所检测到的不同位点的有效等位基因数(Ne)范围为1.00~1.99，平均为1.48±0.04；基因多样度(H)为0.00~0.49，平均为0.29±0.02；Shannon信息指数(I)为0.00~0.69，平均为0.44±0.02。本研究分析了西藏核桃优良单株的遗传多样性和亲缘关系，建立了它们的指纹图谱。

       

      Abstract: In order to deeply understand the genetic background and the genetic diversity of Tibet walnut germplasm resources, FISHAFLP was conducted using 9 EcoRⅠ+3/MseⅠ+3 primer combinations to analyse 11 walnut (Juglans regia L.) advanced trees from Tibet at genomic DNA level. Totally 1 103 AFLP fragments were obtained, in which 1 076 were polymorphic and the average percentage of polymorphic bands (ppb) was 97.67%. The value of effective number of alleles (Ne) ranged from 1.00 to 1.99 with an average of 1.48±0.04; Neis gene diversity (H) ranged from 0.00 to 0.49 with an average of 0.29±0.02 and Shannons information index (I) ranged from 0.00 to 0.69 with an average of 0.44±0.02The genetic diversity and sibship among walnut advanced trees in Tibet were investigated and their fingerprintings were established in the research.

       

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