沙冬青A20/AN1锌指蛋白基因序列及表达分析

Sequence analysis and expression pattern of AmSTZF encoding an A20/AN1 zinc finger protein in Ammopiptanthus mongolicus.

摘要: A20/AN1锌指蛋白普遍存在于真核生物中，在胁迫响应引起的信号传导过程中发挥着重要作用。本文利用生物信息学和RT-PCR方法从低温干旱胁迫的沙冬青叶片组织中分离锌指蛋白基因AmSTZF，该基因编码一条含172个氨基酸残基的多肽，含A20和AN1两个锌指蛋白保守结构域，属A20/AN1锌指蛋白。生物信息学分析显示该蛋白定位于细胞质中，具有磷酸化位点，参与细胞转录调控。RTPCR分析表明：低温和干旱胁迫处理的沙冬青叶片中，AmSTZF的表达增强，初步推测该基因与沙冬青低温、干旱胁迫响应相关。

Abstract: A20/AN1 zinc finger protein widely exists in eukaryote and plays a central role in signal transduction pathway involved in stress response.We cloned zinc finger gene AmSTZF from cold and droughttreated Ammopiptanthus mongolicus leaves by bioinformatics and RTPCR. The cDNA of AmSTZF encodes a polypeptide with 172 amino acids,which contains A20 and AN1 conserved domains and belongs to A20/AN1 zinc finger protein.Bioinformatics predicts that AmSTZF has phosphorylation sites, exists in cytoplasm and is involved in the regulation of gene transcription.RTPCR analysis shows that the expression of AmSTZF is upregulated under cold-and drought-stresses and that AmSTZF is related to the response to drought and chilling stresses.