Abstract: To evaluate the application activity of pyrolysis condensate liquid of disused woodbased board, bacteriostatic experiments of particle board and medium density fiber board pyrolysis condensate liquids obtained under different pyrolysis conditions were taken with bacteriostatic circle method in this paper. Liquids components were analyzed by GC-MS. Woodbased board pyrolysis condensate liquids had obvious bacteriostatic effects to Staphylococcus aureus and Escherichia coli with the diameters of bacteriostatic circle up to 23.48 and 20.54 mm. GC-MS analysis showed that there were more nitrogen compounds and less acids, ketones, phenols and esters in woodbased board pyrolysis condensate liquid than wood vinegar, which might make inhibition rate of woodbased board pyrolysis condensate liquid weaker to S. aureus and E. coli. Even though the bacterostatic effect is weaker than bamboo vinegar and wood vinegar, disused woodbased board pyrolysis condensate liquid with good bacteriostatic effect and wide range of sources is a valuable material to be developed.