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    程嘉莉, 马江, 肖爱华, 朱仲龙, 桑子阳, 马履一. 红花玉兰挥发油萃取及抗氧化、抗菌活性研究[J]. 北京林业大学学报, 2020, 42(2): 96-105. DOI: 10.12171/j.1000-1522.20190249
    引用本文: 程嘉莉, 马江, 肖爱华, 朱仲龙, 桑子阳, 马履一. 红花玉兰挥发油萃取及抗氧化、抗菌活性研究[J]. 北京林业大学学报, 2020, 42(2): 96-105. DOI: 10.12171/j.1000-1522.20190249
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    Cheng Jiali, Ma Jiang, Xiao Aihua, Zhu Zhonglong, Sang Ziyang, Ma Lüyi. Extraction, antioxidant and antibacterial activities of essential oils from Magnolia wufengensis[J]. Journal of Beijing Forestry University, 2020, 42(2): 96-105. DOI: 10.12171/j.1000-1522.20190249
    Citation: Cheng Jiali, Ma Jiang, Xiao Aihua, Zhu Zhonglong, Sang Ziyang, Ma Lüyi. Extraction, antioxidant and antibacterial activities of essential oils from Magnolia wufengensis[J]. Journal of Beijing Forestry University, 2020, 42(2): 96-105. DOI: 10.12171/j.1000-1522.20190249
    shu

    红花玉兰挥发油萃取及抗氧化、抗菌活性研究

    Extraction, antioxidant and antibacterial activities of essential oils from Magnolia wufengensis

      摘要
    • 摘要:
      目的研究红花玉兰花蕾挥发油的萃取工艺，并对其化学组成、抗氧化及抗菌活性进行鉴定和评价，旨在为红花玉兰花蕾挥发油的萃取建立一套可行的工艺体系，并为其后续的开发应用提供技术支持和理论参考。
      方法以‘娇红1号’红花玉兰阴干花蕾为原料，在单因素试验基础上，采用响应面分析法优化超临界CO2萃取工艺，结合自动化质谱解卷积和保留指数对最佳工艺条件下得到的挥发油进行成分定性和定量；采用DPPH自由基清除法和还原能力法评价挥发油抗氧化活性，纸片扩散法和微量肉汤稀释法评价挥发油抗菌活性。
      结果阴干预处理条件下，红花玉兰花蕾挥发油萃取的最佳条件为萃取温度47 ℃，萃取压力40.7 MPa，动态萃取时间102 min，该条件下挥发油的萃取率为（1.217 ± 0.014）%。GC-MS共鉴定出30种化学成分，占检测到的挥发油总量的70.65%。DPPH自由基清除法测定得到挥发油IC50值为12.82 mg/mL AEAC值为198.64 mg，表明挥发油具有一定的抗氧化活性，还原力测定也得到了这一结果。抗菌试验表明，挥发油对4种供试菌抑制作用强弱依次为金黄色葡萄球菌 > 枯草芽孢杆菌 > 大肠杆菌 > 鼠伤寒沙门氏菌。
      结论首次建立了红花玉兰阴干花蕾挥发油的超临界CO2萃取工艺，确定了该工艺条件下挥发油的主要成分，并验证其具有一定的抗氧化和抗菌活性。

       

      Abstract:
      ObjectiveThe aim of this study is to establish a set of reliable process system for the extraction of essential oils from Magnolia wufengensis buds and provide technical support as well as theoretical reference for its subsequent development and application. The extraction process of essential oils from M. wufengensis buds was studied, and chemical compositions, antioxidant and antibacterial activities were identified and evaluated.
      MethodBased on single-factor experiment, the supercritical CO2 fluid extraction conditions of essential oils from air-dried buds of M. wufengensis ‘Jiaohong No.1’ were optimized by response surface methodology. Components of essential oils were qualitatively and quantitatively analyzed by automated mass spectral deconvolution and identification system combined with retention index. DPPH free radical scavenging method and reducing power method were used to analyze the antioxidant activity of essential oils. Disk diffusion method and broth microdilution method were used to measure the antibacterial activities.
      ResultUnder the air-dried pretreatment condition, the optimum processing conditions for extracting essential oils from M. wufengensis were as follows: extraction temperature 47 ℃, extraction pressure 40.7 MPa, dynamic extraction time 102 min. The extraction yield of essential oils under this condition was (1.217 ± 0.014)%. A total of 30 components were identified, accounting for 70.65% of the total components. DPPH free radical scavenging method showed that the IC50 value of essential oils was 12.82 mg/mL, and the AEAC value was 198.64 mg. The essential oils had certain antioxidant activity, and the reducing power method showed the same results. The inhibitory effect of essential oils on the four tested bacteria was in order of Staphylococcus aureus > Bacillus subtilis > Escherichia coli > Salmonella typhimurium.
      ConclusionA feasible supercritical CO2 extraction process of the essential oils from the air-dried buds of M. wufengensis was established for the first time. Furthermore, the main components of the essential oils were identified and its antioxidant and antibacterial activities were verified.

       

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