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    武帅, 姜礅, 马庆辉, 谭明涛, 赵佳齐, 刘晓霞, 孟昭军, 严善春. 丛枝菌根真菌对银中杨叶片物质代谢及化学防御的影响[J]. 北京林业大学学报, 2021, 43(5): 86-92. DOI: 10.12171/j.1000-1522.20200172
    引用本文: 武帅, 姜礅, 马庆辉, 谭明涛, 赵佳齐, 刘晓霞, 孟昭军, 严善春. 丛枝菌根真菌对银中杨叶片物质代谢及化学防御的影响[J]. 北京林业大学学报, 2021, 43(5): 86-92. DOI: 10.12171/j.1000-1522.20200172
    Wu Shuai, Jiang Dun, Ma Qinghui, Tan Mingtao, Zhao Jiaqi, Liu Xiaoxia, Meng Zhaojun, Yan Shanchun. Effects of arbuscular mycorrhizal fungi on metabolism and chemical defense of Populus alba × P. berolinensis leaves[J]. Journal of Beijing Forestry University, 2021, 43(5): 86-92. DOI: 10.12171/j.1000-1522.20200172
    Citation: Wu Shuai, Jiang Dun, Ma Qinghui, Tan Mingtao, Zhao Jiaqi, Liu Xiaoxia, Meng Zhaojun, Yan Shanchun. Effects of arbuscular mycorrhizal fungi on metabolism and chemical defense of Populus alba × P. berolinensis leaves[J]. Journal of Beijing Forestry University, 2021, 43(5): 86-92. DOI: 10.12171/j.1000-1522.20200172

    丛枝菌根真菌对银中杨叶片物质代谢及化学防御的影响

    Effects of arbuscular mycorrhizal fungi on metabolism and chemical defense of Populus alba × P. berolinensis leaves

    • 摘要:
        目的   探究两种丛枝菌根真菌摩西球囊霉(GM)和根内球囊霉(GI)对银中杨物质代谢及化学防御的影响。
        方法   本研究采用孢子含量均为15 个/g的GM、GI基质,分别称取20 g孢子基质与1.3 kg灭菌土混合均匀制成混合基质,将银中杨扦插苗移栽至混合基质中。在丛枝菌根真菌侵染率达到最高时(第90天),分析银中杨叶片内N、P元素、营养物质和次生代谢产物含量,以及防御蛋白活性。
        结果   GM和GI处理组银中杨,叶片内N和P、可溶性蛋白质含量均显著高于对照组(P < 0.05),可溶性糖含量显著低于对照组(P < 0.05),淀粉含量与对照组差异不显著(P > 0.05)。次生代谢产物木质素、黄酮、总酚和单宁含量均显著高于对照组(P < 0.05)。防御蛋白苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、胰蛋白酶抑制剂(TI)和胰凝乳蛋白酶抑制剂(CI)活性均显著高于对照组(P < 0.05)。
        结论   GM和GI能促进银中杨物质代谢,叶片内营养元素N、P和可溶性蛋白含量升高,可溶性糖含量降低,改变叶片品质,改善其耐受性;增强叶部化学防御能力,次生代谢产物含量和防御蛋白活性增加,改变银中杨对叶部害虫的抗性。因此,丛枝菌根真菌最终能否增强银中杨的抗虫性还有待进一步生物测定试验继续研究。

       

      Abstract:
        Objective   This study aims to investigate the effects of two arbuscular mycorrhizal fungi, Glomus mosseae (GM) and Glomus intraradices (GI) on the metabolism and chemical defense of Populus alba × P. berolinensis leaves.
        Method   In this study, each poplar seedling cut was planted into a pot filled with 1.3 kg sterilized soil mixed with 20 g fungal substrate containing 15 spore/g of either GM or GI. The contents of N, P elements, nutrients and secondary metabolites, as well as the activity of defense proteins in leaves of the poplar seedlings were analyzed on the 90th day after the planting when the fungal infection rate reached the peak.
        Result   The contents of N, P elements and soluble proteins in the poplar leaves from the GM or GI treatment group were significantly higher than those from the control group (P < 0.05), whereas the soluble sugar content was significantly lower (P < 0.05). There were no significant differences in starch contents between the GM or GI treatment group and the control group (P > 0.05). The contents of secondary metabolites, lignin, flavonoids, total phenols and tannins were significantly higher in the GM or GI treatment group than those in the control group (P < 0.05). The activities of defense proteins, phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), trypsin inhibitor (TI) and chymotrypsin inhibitor (CI) were all significantly higher in the treatment groups than those in the control group (P < 0.05).
        Conclusion   The above results indicate that GM and GI might promote the metabolism of Populus alba × P. berolinensis leaves, including increase in the contents of N, P elements and soluble proteins and decrease in the content of soluble sugars, resulting in potential changes in leaf quality, and improvement of leaf tolerance. GM or GI infestation seems to increase the chemical defense ability of the poplar leaves, such as the increase of secondary metabolite contents and activity of defense proteins, thus might affect the resistance of Populus alba × P. berolinensis leaves to pests. However, further research is surely needed to determine whether these two arbuscular mycorrhizal fungi can significantly enhance the resistance of Populus alba × P. berolinensis to pest insects.

       

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