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    李丽菁, 张智韦, 薛云, 张嘉航, 韩烈保, 许立新. 低温胁迫对日本结缕草叶绿素代谢的影响[J]. 北京林业大学学报, 2022, 44(2): 91-99. DOI: 10.12171/j.1000-1522.20200400
    引用本文: 李丽菁, 张智韦, 薛云, 张嘉航, 韩烈保, 许立新. 低温胁迫对日本结缕草叶绿素代谢的影响[J]. 北京林业大学学报, 2022, 44(2): 91-99. DOI: 10.12171/j.1000-1522.20200400
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    Li Lijing, Zhang Zhiwei, Xue Yun, Zhang Jiahang, Han Liebao, Xu Lixin. Effects of low temperature stress on chlorophyll metabolism of Zoysia japonica[J]. Journal of Beijing Forestry University, 2022, 44(2): 91-99. DOI: 10.12171/j.1000-1522.20200400
    Citation: Li Lijing, Zhang Zhiwei, Xue Yun, Zhang Jiahang, Han Liebao, Xu Lixin. Effects of low temperature stress on chlorophyll metabolism of Zoysia japonica[J]. Journal of Beijing Forestry University, 2022, 44(2): 91-99. DOI: 10.12171/j.1000-1522.20200400
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    低温胁迫对日本结缕草叶绿素代谢的影响

    Effects of low temperature stress on chlorophyll metabolism of Zoysia japonica

      摘要
    • 摘要:
        目的  日本结缕草是具有众多优良性状，被广泛应用的暖季型草坪草。通过对其叶绿素合成及降解的研究，探究日本结缕草秋冬季节在我国北方地区较早出现叶片脱绿现象的内源机理。
        方法  对日本结缕草进行14 h光照（4 ℃）、10 h黑暗（2 ℃）的循环低温处理，并对叶绿素、叶绿素合成与降解途径中重要酶的活性、中间产物含量以及叶绿素代谢相关基因表达量进行测定。
        结果  研究发现在低温胁迫处理下，日本结缕草叶片表观形态上有明显变化，严重枯黄且卷曲；叶绿素、叶绿素a、叶绿素b的浓度显著下降，叶绿素a与b的比值减小；5-氨基乙酰丙酸盐脱水酶、胆色素原脱氨酶（PBGD）活性和单卟啉胆色素原（PBG）含量显著低于对照组；脱镁鳌合酶（MDCase）活性显著高于对照组；叶绿素合成基因（除ZjMgCH2）均下调表达；叶绿素降解相关基因（除ZjNYC1）均比对照组较高表达。
        结论  低温胁迫下，日本结缕草叶绿素合成减弱，同时加速了叶绿素的降解。此外，验证了MDCase是日本结缕草叶绿素降解途径中的关键因子。本研究为进一步解决日本结缕草滞绿性状提供了理论基础，也对培育日本结缕草滞绿品种育种工作具有重要意义。

       

      Abstract:
        Objective  Zoysia japonica is a warm season lawn grass, which has many excellent characters and is widely used. One of the limiting factors for Z. japonica is its early leaf de-greening in late autumn and early winter in the north. The objective of this study was to investigate the underlying mechanism of leaf de-greening process of Z. japonica in response to low temperature stress.
        Method  In this experiment, Z. japonica was exposed to light for 14 h (4 ℃) and dark for 10 h (2 ℃) in a growth chamber to simulate cold stress. Chlorophyll content, the activities of key enzymes in chlorophyll synthesis and degradation pathway and the expression of genes related to chlorophyll metabolism were studied.
        Result  Z. japonica leaves turned yellow and curly under low temperature stressed conditions. The concentrations of chlorophyll, chlorophyll a, chlorophyll b , the ratio of chlorophyll a to chlorophyll b , the activities of ALAD, PBGD and the content of PBG in low temperature treatment group were significantly lower than CK. The activity of MDCase increased significantly. Chlorophyll synthesis related genes (except ZjMgCH2) were down-expressed and chlorophyll degradation related genes (except ZjNYC1) were high-expressed.
        Conclusion  Chlorophyll synthesis decreases and chlorophyll degradation increases in Z. japonica under low temperature stress. In addition, MDCase is proved to be the key factor in the chlorophyll degradation pathway of Z. japonica. This study provides a theoretical basis for understanding mechanisms underlying the stay-green characters of Z. japonica. At the same time, it has great significance to breeding variety of Z. japonica with stay-green character.

       

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