高级检索
    宋婷婷, 梁楠松, 吕义品, 崔靖弘, 于磊, 赵福江, 徐亮, 詹亚光. 水曲柳FmPLT基因家族的鉴定及表达分析[J]. 北京林业大学学报, 2022, 44(2): 11-21. DOI: 10.12171/j.1000-1522.20210105
    引用本文: 宋婷婷, 梁楠松, 吕义品, 崔靖弘, 于磊, 赵福江, 徐亮, 詹亚光. 水曲柳FmPLT基因家族的鉴定及表达分析[J]. 北京林业大学学报, 2022, 44(2): 11-21. DOI: 10.12171/j.1000-1522.20210105
    shu
    Song Tingting, Liang Nansong, Lü Yipin, Cui Jinghong, Yu Lei, Zhao Fujiang, Xu Liang, Zhan Yaguang. Identification and expression analysis of FmPLT gene family of Fraxinus mandschurica[J]. Journal of Beijing Forestry University, 2022, 44(2): 11-21. DOI: 10.12171/j.1000-1522.20210105
    Citation: Song Tingting, Liang Nansong, Lü Yipin, Cui Jinghong, Yu Lei, Zhao Fujiang, Xu Liang, Zhan Yaguang. Identification and expression analysis of FmPLT gene family of Fraxinus mandschurica[J]. Journal of Beijing Forestry University, 2022, 44(2): 11-21. DOI: 10.12171/j.1000-1522.20210105
    shu

    水曲柳FmPLT基因家族的鉴定及表达分析

    Identification and expression analysis of FmPLT gene family of Fraxinus mandschurica

      摘要
    • 摘要:
        目的  PLT转录因子家族能够参与植物的再生过程，并且在植物的生长发育及器官建成中发挥着重要作用，本研究旨在探究PLT转录因子在水曲柳生长发育及非生物胁迫中发挥的作用，以期为林木优良基因选育工作提供更多思路。
        方法  通过同源序列比对和基因克隆等方法对FmPLTs家族的成员进行鉴定及分析，之后对FmPLTs成员进行了详细的生物信息学分析，包括理化性质、系统进化关系和基因结构等；并进一步分析了FmPLTs成员在不同组织及种子萌发过程中的基因表达特征，以及其在非生物胁迫（氯化钠、聚乙二醇6000、碳酸氢钠和低温4 ℃）和植物激素处理（脱落酸、赤霉素、生长素、水杨酸和茉莉酸甲酯下的诱导表达情况。
        结果  水曲柳基因组中含有9个PLT基因家族成员，分别命名为FmPLT2、FmPLT3、FmPLT4、FmPLT4A、FmPLT5、FmPLT5A、FmPLT7、FmPLT8和FmPLT9。系统进化关系将其分为5组；其在根中表达量最高，在叶中表达量最低；在种子萌发第4 天子叶变绿，下胚轴伸长，此时FmPLTs成员的表达量出现峰值；而在不同激素及非生物胁迫条件下，FmPLTs对低温、干旱、盐胁迫以及MeJA的处理响应明显。
        结论  FmPLTs参与水曲柳种子萌发及器官发育，并能响应植物激素信号，同时积极参与植物非生物胁迫耐受性的调控，包括低温、高盐和干旱胁迫等；本研究初步揭示了水曲柳FmPLT家族成员响应植物激素和非生物胁迫的表达模式，同时为深入分析FmPLT基因家族基本功能及其调控机制奠定了基础。

       

      Abstract:
        Objective  PLT transcription factor family plays an important role in plant growth and organ formation and participates in the regeneration progress. This paper aims to explore the function of PLT transcription factors in the growth and abiotic stress in Fraxinus mandshurica, in order to provide more ideas for forest tree gene selection work.
        Method  By using homologous alignment and cloning techniques, we obtained and identified all members of FmPLT gene family. We conducted detailed bioinformatics analysis of FmPLTs, including physicochemical properties, phylogenetic relationships and gene structure, etc. Besides, we measured the gene expression levels of FmPLTs members in different tissues and seeds during germination, and the expression levels under abiotic stresses (NaCl, PEG6000, NaHCO3, and low temperature 4 ℃) and signal hormone treatmentes (ABA, GA3, IAA, SA and MeJA).
        Result  We revealed that the F. mandshurica genome contained 9 members, which were named as FmPLT2, FmPLT3, FmPLT4, FmPLT4A, FmPLT5, FmPLT5A, FmPLT7, FmPLT8 and FmPLT9, respectively, based on phylogenetic relationship, that could be further divided into 5 groups. The expression of PLT family members was the highest in roots and the lowest in leaves. On the fourth day of seed germination, cotyledons turned green, hypocotyls elongated, most members showed highest expression levels, in response to different hormones and abiotic stresses, FmPLTs showed significant responses to low temperature, drought, salt stress and MeJA treatment.
        Conclusion  FmPLTs are involved in seed germination and organ development of Fraxinus mandshurica, response to plant hormone signaling, and actively participate in the regulation of plant abiotic stress tolerance, including low temperature, high salt and drought stress. This study preliminarily reveals that the expression pattern of FmPLTs in response to both signal hormone treatmentes and abiotic stress, and lays a foundation for in-depth analysis of the basic functions and regulatory mechanisms of FmPLT gene family.

       

      • HTML全文
      • 参考文献(39)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回