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    张运城, 周长虹, 钮世辉, 李伟. 针叶树GID1同源基因分离鉴定与功能预测[J]. 北京林业大学学报, 2015, 37(5): 40-48. DOI: 10.13332/j.1000-1522.20140255
    引用本文: 张运城, 周长虹, 钮世辉, 李伟. 针叶树GID1同源基因分离鉴定与功能预测[J]. 北京林业大学学报, 2015, 37(5): 40-48. DOI: 10.13332/j.1000-1522.20140255
    ZHANG Yun-cheng, ZHOU Chang-hong, NIU Shi-hui, LI Wei. Isolation and identification of GID1 orthologous gene in conifers and its function prediction.[J]. Journal of Beijing Forestry University, 2015, 37(5): 40-48. DOI: 10.13332/j.1000-1522.20140255
    Citation: ZHANG Yun-cheng, ZHOU Chang-hong, NIU Shi-hui, LI Wei. Isolation and identification of GID1 orthologous gene in conifers and its function prediction.[J]. Journal of Beijing Forestry University, 2015, 37(5): 40-48. DOI: 10.13332/j.1000-1522.20140255

    针叶树GID1同源基因分离鉴定与功能预测

    Isolation and identification of GID1 orthologous gene in conifers and its function prediction.

    • 摘要: GID1作为赤霉素(GA)受体蛋白,是GA信号通路的重要组成部分,其编码基因GID1在被子植物中已经被广泛克隆,但在针叶树种中的研究十分滞后。为了分离针叶树GA受体GID1基因并推测其功能,本研究以拟南芥GID1s序列为探针,在油松高质量参考转录组内筛选并鉴别出了油松GID1直系同源基因;基于该基因序列同源克隆了樟子松、白皮松、赤松GID1基因,通过BLAST获得了日本落叶松、火炬松、白云杉与挪威云杉的GID1-like基因;对针叶树GID1基因进行序列保守性、蛋白结构和组织表达活性分析。结果表明:针叶树种很可能只含有一个GID1基因,该基因在针叶树中具有很高的保守性;虽然与被子植物GID1之间的序列一致性较低,但其保持GA亲和活性所必需的氨基酸残基十分保守,与其下游DELLA蛋白相互作用的功能域与结构同样十分保守,推测其在针叶树GA信号转导中具有受体功能;表达分析显示GID1在挪威云杉不同组织和油松雌雄球花不同发育阶段间表达较为稳定,表明GID1可能广泛参与这些组织的发育过程,针叶树GA信号调控通路中GA受体的转录调控可能并不是核心调控机制。研究结果为GID1基因在针叶树生长发育过程中的分子调控机制研究奠定了基础。

       

      Abstract: GID1 as the gibberellins (GA) receptor is an essential component of GA signaling pathway.The GID1 genes have been cloned widely from angiosperms,but researches on them in conifers are scarcely documented.We obtained and identified the orthologous gene of GID1 from Pinus tabuliformis transcriptome with AtGID1s probe sequence.Based on this sequence,the GID1 genes were cloned from P.sylvestris var. mongolica,P.bungeana and P.densiflora and the GID1-like genes in P.taeda,Picea glauca,P.abies and Larix kaempferi were also obtained from public database.The conservation of amino acid sequences,protein structures and expression levels of coniferous GID1s were analyzed.The result showed that there probably is only one GID1 with highly conserved sequence in conifers.Although the identity of GID1s is low between conifers and angiosperms,they have highly similar amino acids which are essential for binding affinity to GA and quite conserved functional domains which can interact with DELLA protein.Thus, these orthologous GID1 genes may have functions of receptor.Expression analysis showed that the GID1 had stable expressions in different organs in P.abies and in different developmental stages of male and female cones in Pinus tabuliformis, indicating that GID1 may widely participate in developmental process of these tissues,and the transcriptional regulation of GID1 might not be the key regulatory mechanism in GA signaling pathway.Our study lays a foundation for the research of molecular mechanism of GID1 in the growth and development of conifers.

       

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