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    王泽伟, 张炎, 齐帅征, 朱军杰, 袁玮, 张亚东, 刘学增, 张金凤. 杂种枫香组织培养再生研究[J]. 北京林业大学学报, 2018, 40(8): 42-49. DOI: 10.13332/j.1000-1522.20170443
    引用本文: 王泽伟, 张炎, 齐帅征, 朱军杰, 袁玮, 张亚东, 刘学增, 张金凤. 杂种枫香组织培养再生研究[J]. 北京林业大学学报, 2018, 40(8): 42-49. DOI: 10.13332/j.1000-1522.20170443
    shu
    Wang Zewei, Zhang Yan, Qi Shuaizheng, Zhu Junjie, Yuan Wei, Zhang Yadong, Liu Xuezeng, Zhang Jinfeng. Tissue culture regeneration of hybrid Liquidambar styraciflua × L. formosana[J]. Journal of Beijing Forestry University, 2018, 40(8): 42-49. DOI: 10.13332/j.1000-1522.20170443
    Citation: Wang Zewei, Zhang Yan, Qi Shuaizheng, Zhu Junjie, Yuan Wei, Zhang Yadong, Liu Xuezeng, Zhang Jinfeng. Tissue culture regeneration of hybrid Liquidambar styraciflua × L. formosana[J]. Journal of Beijing Forestry University, 2018, 40(8): 42-49. DOI: 10.13332/j.1000-1522.20170443
    shu

    杂种枫香组织培养再生研究

    Tissue culture regeneration of hybrid Liquidambar styraciflua × L. formosana

      摘要
    • 摘要:
      目的枫香具有适应性强、生长速度快、观赏价值高等特点，既可以作为用材树种又可以作为优良的园林绿化树种。北美枫香能够与中国枫香杂交并可产生有杂种优势的后代，但是杂种枫香的快速繁殖技术和遗传转化体系还有待完善。
      方法本研究以北美枫香为母本，中国枫香为父本进行杂交，选用子一代杂种枫香的子叶、子叶节以及下胚轴为外植体材料建立高效的组培再生体系，并对3种外植体的分化能力进行比较。
      结果(1) 子叶和子叶节外植体最佳芽诱导培养基为0.2 mg/L TDZ+0.1 mg/L NAA的WPM培养基，而下胚轴外植体最佳芽诱导培养基为0.1 mg/L TDZ+0.1 mg/L NAA的WPM培养基。(2)3种外植体在0.5 mg/L 6-BA+0.1 mg/L NAA+0.8 mg/L GA不加琼脂的WPM芽伸长液体培养基中获得最高的平均不定芽诱导数。(3)不定芽在2.0 mg/L IBA+0.1 mg/L NAA的WPM培养基中生根，生根率达到100%。(4)不定芽生根培养2个月后长为完整植株，移栽成活率在90%以上。
      结论(1) 杂种枫香3种外植体的分化能力依次为：子叶节>子叶>下胚轴，其中子叶节的分化能力显著高于子叶和下胚轴。(2)TDZ能够有效促进不定芽的诱导，GA对不定芽的伸长具有显著的影响。(3)杂种枫香外植体在固体培养基中获得了最高的不定芽诱导率，在液体培养基中获得了最高不定芽平均数量，而且液体培养中未出现玻璃化。本研究为杂种枫香新品种的快速繁育以及遗传转化体系的建立奠定了良好的基础。

       

      Abstract:
      ObjectiveLiquidambar sp. has strong adaptability, fast growth, high ornamental values, it can be used as commercial tree species and landscaping tree species. Liquidambar styraciflua can be interfertiled with Liquidambar fomorsana, and their offspring can show robust growth. However, the rapid propagation technology and genetic transformation system of hybrid sweetgum need to be improved.
      MethodIn this study, using Liquidambar styraciflua as female parent and Liquidambar fomorsana as male parent to obtain hybrid sweetgum, and hybrid sweetgum cotyledon, cotyledon node and hypocotyl explants were used to establish an efficient tissue culture regeneration system, we also compared the differentiation ability of the three explants.
      Result(1) The best bud induction medium of cotyledon and cotyledon node explants was 0.2 mg/L TDZ+0.1 mg/L NAA basal WPM, but the best bud induction medium of hypocotyl explants was 0.1 mg/L TDZ+0.1 mg/L NAA basal WPM; (2) Shoot elongation liquid medium contained 0.5 mg/L 6-BA+0.1 mg/L NAA+0.8 mg/L GA without agar WPM performed the highest average adventitious bud induction number for three explant types; (3) Adventitious buds were rooted in 2.0 mg/L IBA+0.1 mg/L NAA WPM medium, and its rooting rate reached 100%;(4) After the adventitious buds being cultured in rooting medium for 2 months, the plantlets were transferred into greenhouse, and the transplant survival rate was more than 90%.
      Conclusion(1) The differentiation abilities of the three explants were as follows: cotyledonary node>cotyledon> hypocotyl, in which the differentiation ability of cotyledonary nodes was significantly higher than cotyledon and hypocotyl; (2) TDZ can effectively promote the induction of adventitious buds, and GA has a significant effect on adventitious shoot elongation; (3) The hybrid sweetgum explants obtained the highest adventitious bud induction rate in the solid medium, the highest number of adventitious buds was obtained in the liquid medium, and no vitrification occurred in the liquid culture. The results of this experiment lay a solid foundation for the rapid propagation of new varieties and the establishment of genetic transformation system of hybrid sweetgum.

       

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