Composition analysis and structural identification of polyphenols from Pinus sylvestris var. mongolica barks

The composition of polyphenols from Pinus sylvestris L. var. mongolica were analyzed and their structures were identified with UV-VIS, IR, HPLC and LC-MS. The UV-VIS results showed that there are absorption peaks in regions of 240-- 280 nm and 300-- 400 nm, indicating that flavonoids exist in the pine polyphenols. Fourier Transform Infrared Spectrum analysis showed that a strong and broad absorption peak at 3 400 cm-1 is generated from stretching and vibration of phenolic hydroxyl Ar-OH and absorption peaks at 1 390-- 1 315 cm-1 due to characteristics of in-plane bending vibration of hydroxyl δO—H, which indicated that hydroxyl groups existed in the pine polyphenols. Combined application of HPLC and LC-MS showed that there are seven components in P. sylvestris L. var. mongolica polyphenols, i.e., catechuic acid, rutin, chlorogenic acid, gallic acid, sinapic acid-dextrose glucoside, peony-3- glucoside or centaurea-3-(6-o-acetyl)-glucose glycoside, and sinapic acid peonidin-3-sophoroside composition.