Kinetics of Cistanche deserticola cells in suspension culture
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Abstract
The growth period of Cistanche deserticola cells in suspension culture is about 25 days.The relationship between specific productivity of phenylethanoid glycosides and cell specific growth rate growth behaved linearly.During the growing period,phosphate and ammonia in culture media were consumed quickly, but nitrate was used slowly.The cell yield coefficients based on sucrose,ammonia and nitrate were 0.48,160 and 6.4 g/g respectively.PeG yield coefficient based on sucrose was 0.055 g/g.The stoichiometrical equation of C.deserticola cells in suspension culture was deduced by the analysis of elemental composition of cells and these yield coefficients.
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