Construction of ISSR identification card of Ulmus pumila Jinye' and analysis of genetic diversity of 8 Ulmus materials.

Inter-simple sequence repeat (ISSR) marker was used to analyze genetic diversity of 8 improved breeds of Ulmus spp. Twelve primers screened out from 78 primers were used for ISSR-PCR. A total of 74 bands were generated, of which 63 bands appeared to be polymorphic, occupied 85% of total. The amplification results of ISSR were analyzed by NTSYSpc 2-10e software, and indicated that there actually existed high genetic diversity at molecular level among the 8 Ulmus materials. The method of amplified band assignment in 1,otherwise 0 was used to establish a ISSR identification card. These 8 improved breeds of Ulmus materials were divided into four groups with ISSR marker by UPGMA method.