‘回归’百合组织培养再生体系的建立

姜婧瑶; 吕博妍; 骆远方; 高雪; 贾桂霞

doi:10.12171/j.1000-1522.20250078

摘要:

目的 ‘回归’百合具有极高的经济价值和观赏价值，建立其高效的组织培养体系至关重要。本研究旨在结合鳞片的高效性和叶片的可持续性优势，构建‘回归’百合愈伤组织途径的再生体系，为其遗传转化和新品种选育提供技术支撑。

方法以百合‘回归’鳞片和叶片为外植体，筛选最适的愈伤组织诱导培养基；探究不同6-BA浓度对不定芽分化的影响，不同蔗糖浓度对小鳞茎膨大的影响，不同活性炭浓度对生根的影响，并开展组培苗移栽试验。

结果（1）适宜‘回归’百合鳞片的愈伤组织诱导培养基为MS＋1.0 mg/L PIC＋0.5 mg/L TDZ，诱导率为（60.98 ± 13.90）% ，愈伤组织面积达（0.62 ± 0.23）cm²；适宜‘回归’百合叶片的愈伤组织诱导培养基为MS + 2.0 mg/L PIC + 0.2 mg/L TDZ，诱导率为（36.83 ± 1.89）% ，愈伤组织面积为（0.34 ± 0.08）cm²。不同外植体间愈伤组织形成与发育存在差异，鳞片外植体诱导愈伤组织的效果更好。（2）含有1.0 mg/L 6-BA与0.2 mg/L NAA的MS培养基对愈伤组织不定芽分化具有较优效果，其愈伤分化率为100%，芽再生系数为8.20 ± 0.60。（3）在MS培养基中添加90 g/L蔗糖可促进小鳞茎质量增长；在根系发育阶段，含2 g/L活性炭和0.2 mg/L NAA的MS培养基为最适生根培养基，移栽成活率高达96.00%。

结论本研究成功构建‘回归’百合愈伤组织高效诱导和再生体系，实现了从愈伤组织诱导到成苗移栽的全周期优化，研究结果将为‘回归’百合遗传转化和新品种推广奠定基础。

Abstract:

Objective The Lilium ‘Regression’ has extremely high economic and ornamental values. Establishing an efficient tissue culture system for it is of great importance. This study aims to integrate the high efficiency of scales and the sustainability of leaves to construct a regeneration system for Lilium ‘Regression’ via the callus tissue pathway, providing technical support for its genetic transformation and new variety breeding.

Method Using scales and leaves of Lilium ‘Regression’ as explants, the optimal callus induction medium was screened. The effects of different 6-BA concentrations on adventitious bud differentiation, different sucrose concentrations on bulblet swelling, different activated carbon concentrations on rooting were investigated, and tissue culture seedling transplantation experiments were carried out.

Result (1) The suitable callus induction medium for scales of ‘Regression’ lily was MS + 1.0 mg/L PIC + 0.5 mg/L TDZ, with an induction rate of (60.98 ± 13.90)% and a callus area of (0.62 ± 0.23) cm². The suitable callus induction medium for leaves of ‘Regression’ lily was MS + 2.0 mg/L PIC + 0.2 mg/L TDZ, with an induction rate of (36.83 ± 1.89)% and a callus area of (0.34 ± 0.08) cm². There were differences in callus formation and development between different explants, among which the scale explants had better effects on callus induction. (2) The MS medium containing 1.0 mg/L 6-BA and 0.2 mg/L NAA had optimal effects on adventitious bud differentiation from callus, with a callus differentiation rate of 100% and a bud regeneration coefficient of 8.20 ± 0.60. Adding 90 g/L sucrose to the MS medium promoted the mass growth of bulblets. In root development stage, the MS medium containing 0.2 mg/L NAA and 2 g/L activated carbon was the optimal rooting medium, and the transplantation survival rate was as high as 96.00%.

Conclusion This study successfully established an efficient induction and regeneration system for callus tissue of Lilium‘Regression’, achieving full-cycle optimization from callus induction to seedling transplantation. The research results will lay foundation for genetic transformation and new variety promotion of Lilium‘Regression’ .

‘回归’百合组织培养再生体系的建立

Establishment of tissue culture and regeneration system for Lilium ‘Regression’