Advances in research on RGS of phytopathogenic filamentous fungi

Han Changzhi; Zhu Youpeng

doi:10.12171/j.1000-1522.20200196

Journal of Beijing Forestry University > 2021 > 43(4): 150-157. > DOI: 10.12171/j.1000-1522.20200196

Han Changzhi, Zhu Youpeng. Advances in research on RGS of phytopathogenic filamentous fungi[J]. Journal of Beijing Forestry University, 2021, 43(4): 150-157. DOI: 10.12171/j.1000-1522.20200196

Citation:

Han Changzhi, Zhu Youpeng. Advances in research on RGS of phytopathogenic filamentous fungi[J]. Journal of Beijing Forestry University, 2021, 43(4): 150-157. DOI: 10.12171/j.1000-1522.20200196

Citation:

Han Changzhi, Zhu Youpeng. Advances in research on RGS of phytopathogenic filamentous fungi[J]. Journal of Beijing Forestry University, 2021, 43(4): 150-157. DOI: 10.12171/j.1000-1522.20200196

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Advances in research on RGS of phytopathogenic filamentous fungi

Han Changzhi^{1, 2,},
Zhu Youpeng^{1, 3}

1.
College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, Yunnan, China
2.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, Yunnan, China
3.
Graduate School of Southwest Forestry University, Kunming 650224, Yunnan, China

More Information

Received Date: June 24, 2020
Revised Date: February 24, 2021
Available Online: March 07, 2021
Published Date: April 29, 2021

Graphical Abstract

Abstract

Abstract

Regulators of G-protein signaling are important negative regulatory factors in G protein signaling pathway, which plays an important role in the realization of mycelial development, sporulation, secondary metabolites, pigment synthesis, pathogenicity and sexual reproduction regulation of fungi. In recent years, with the deepening of research on RGS of plant pathogenic filamentous fungi, a large number of academic reports have been produced. However, systematic comparative analysis of RGS in model fungi and phytopathogenic filamentous fungi has not been reported. In this study, the structure, classification and function of RGS of model fungi and plant pathogenic filamentous fungi were compared and analyzed. At the same time, through SMART conservative domain, secondary structure composition and genetic relationship analysis, RGS proteins in plant pathogenic filamentous fungi and model fungi were confirmed to have conservative RGS domain and similar secondary structure composition, also RGS proteins were divided into 6 categories according to sequence homology, and RGS proteins with different domains were clustered, respectively. The results showed that RGS proteins had certain conservativeness in functional performance in different fungi. In addition, the number and types of RGS in plant pathogenic filamentous fungi were found to be more than that of model organisms. The results show that RGS proteins have certain uniqueness in the functions in different fungi. The above research provides an important theoretical basis for further research on the mechanism of RGS in plant pathogenic filamentous fungi and the relationship between RGS in plant pathogenic filamentous fungi and other model organisms.
- filamentous fungi,
- RGS,
- domain,
- function,
- review

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References (39)

References

[1]	Wilkinson S W, Magerøy M H, Sánchez A L, et al. Surviving in a hostile world: plant strategies to resist pests and diseases[J]. Annual Review of Phytopathology, 2019, 57: 505−529. doi: 10.1146/annurev-phyto-082718-095959
[2]	韩长志. 植物病原丝状真菌G蛋白偶联受体的研究进展[J]. 微生物学通报, 2015, 42(2):374−383. Han C Z. Advance in functional research of G protein-coupled receptors in phytopathogenic filamentous fungi[J]. Microbiology China, 2015, 42(2): 374−383.
[3]	McPherson K B, Leff E R, Li M H, et al. Regulators of G-protein signaling (RGS) proteins promote receptor coupling to G-protein-coupled inwardly rectifying potassium (GIRK) channels[J]. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience, 2018, 38(41): 8737−8744. doi: 10.1523/JNEUROSCI.0516-18.2018
[4]	Chan R K, Otte C A. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones[J]. Molecular and Cellular Biology, 1982, 2(1): 11−20. doi: 10.1128/MCB.2.1.11
[5]	Dixit G, Kelley J B, Houser J R, et al. Cellular noise suppression by the regulator of G protein signaling Sst2[J]. Molecular Cell, 2014, 55(1): 85−96. doi: 10.1016/j.molcel.2014.05.019
[6]	Venkatapurapu S P, Kelley J B, Dixit G, et al. Modulation of receptor dynamics by the regulator of G protein signaling Sst2[J]. Molecular Biology of the Cell, 2015, 26(22): 4124−4134. doi: 10.1091/mbc.E14-12-1635
[7]	Kwon N J, Park H S, Jung S, et al. The putative guanine nucleotide exchange factor RicA mediates upstream signaling for growth and development in aspergillus[J]. Eukaryotic Cell, 2012, 11(11): 1399−1412. doi: 10.1128/EC.00255-12
[8]	Whittington A, Wang P. The RGS protein Crg2 is required for establishment and progression of murine pulmonary cryptococcosis[J]. Medical Mycology, 2011, 49(3): 263−275. doi: 10.3109/13693786.2010.512618
[9]	张海峰. 稻瘟病菌G蛋白及MAPK信号途径相关基因的功能分析[D]. 南京: 南京农业大学, 2011. Zhang H F. Functional analysis of G protein and MAPK signaling pathway associated genes in Magnaporthe oryzae[D]. Nanjing: Nanjing Agricultural University, 2011.
[10]	Mukherjee M, Kim J E, Park Y S, et al. Regulators of G-protein signalling in Fusarium verticillioides mediate differential host-pathogen responses on nonviable versus viable maize kernels[J]. Molecular Plant Pathology, 2011, 12(5): 479−491. doi: 10.1111/j.1364-3703.2010.00686.x
[11]	Park A R, Cho A R, Seo J A, et al. Functional analyses of regulators of G protein signaling in Gibberella zeae[J]. Fungal Genetics and Biology, 2012, 49(7): 511−520. doi: 10.1016/j.fgb.2012.05.006
[12]	Wang Y C, Geng Z Y, Jiang D W, et al. Characterizations and functions of regulator of G protein signaling (RGS) in fungi[J]. Applied Microbiology and Biotechnology, 2013, 97(18): 7977−7987. doi: 10.1007/s00253-013-5133-1
[13]	乐鑫怡. 稻瘟病菌RGS家族蛋白RGS结构域的功能解析及Dynamin家族蛋白MoDnm2、MoDnm3的生物学功能研究[D]. 南京: 南京农业大学, 2017. Le X Y. Functional analysis of RGS domain in RGS protein family and dynamin protein MoDnm2, MoDnm3 in Magnaporthe oryzae during development and pathogenicity[D]. Nanjing: Nanjing Agricultural University, 2017.
[14]	徐爽, 柯智健, 张凯, 等. 胶孢炭疽菌G蛋白信号调控因子CgRGS3的生物学功能[J]. 植物保护学报, 2018, 45(4):827−835. Xu S, Ke Z J, Zhang K, et al. Biological function of a regulator of G-protein signaling CgRGS3 in Colletotrichum gloeosporioides[J]. Journal of Plant Protection, 2018, 45(4): 827−835.
[15]	吴曼莉, 李晓宇, 张楠, 等. 胶孢炭疽菌CgRGS2基因的克隆及生物学功能[J]. 微生物学报, 2017, 57(1):66−76. Wu M L, Li X Y, Zhang N, et al. Gene cloning and biological function of CgRGS2 in Colletotrichum gloeosporioides[J]. Acta Microbiologica Sinica, 2017, 57(1): 66−76.
[16]	赵勇, 王云川, 蒋德伟, 等. 真菌G蛋白信号调控蛋白的功能研究进展[J]. 微生物学通报, 2014, 41(4):712−718. Zhao Y, Wang Y C, Jiang D W, et al. Advances in functional research of RGS proteins in fungi[J]. Microbiology China, 2014, 41(4): 712−718.
[17]	邢新婧. 坚粘孢单顶孢MAPK和RGS4蛋白的功能初步研究[D]. 昆明: 云南大学, 2017. Xing X J. Preliminary study on functions of MAPK and RGS4 proteins in Dactylellina haptotyla[D]. Kunming: Yunnan University, 2017.
[18]	朱小彬, 朱霞, 于一帆, 等. G蛋白信号转导调节蛋白(RGS)研究进展[J]. 中国农学通报, 2014, 30(6):248−253. Zhu X B, Zhu X, Yu Y F, et al. Advances of research on regulators of G protein signaling(RGS proteins)[J]. Chinese Agricultural Science Bulletin, 2014, 30(6): 248−253.
[19]	O’Brien J B, Wilkinson J C, Roman D L. Regulator of G-protein signaling (RGS) proteins as drug targets: progress and future potentials[J]. Journal of Biological Chemistry, 2019, 294(49): 18571−18585. doi: 10.1074/jbc.REV119.007060
[20]	王心睿, 杨红, 廖之君. DEP结构域的结构与功能[J]. 生命的化学, 2015, 35(2):264−271. Wang X R, Yang H, Liao Z J. Structure and function of the DEP domain[J]. Chemistry of Life, 2015, 35(2): 264−271.
[21]	潘华珍, 许彩民. 蛋白质PX结构域的结构和功能[J]. 生命的化学, 2002(5):395−397. Pan H Z, Xu C M. Structure and function of the PX domain[J]. Chemistry of Life, 2002(5): 395−397.
[22]	Willars G B. Mammalian RGS proteins: multifunctional regulators of cellular signalling[J]. Seminars in Cell & Developmental Biology, 2006, 17(3): 363−376.
[23]	祝友朋, 韩长志. 植物病原丝状真菌寄生性与RGS蛋白的关系研究[J]. 华中农业大学学报, 2020, 39(6):23−29. Zhu Y P, Han C Z. Relationship between parasitism and RGS protein in plant pathogenic filamentous fungi[J]. Journal of Huazhong Agricultural University, 2020, 39(6): 23−29.
[24]	Dohlman H G, Song J, Ma D, et al. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit)[J]. Molecular and Cellular Biology, 1996, 16(9): 5194−5209. doi: 10.1128/MCB.16.9.5194
[25]	Chasse S A, Flanary P, Parnell S C, et al. Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae[J]. Eukaryotic Cell, 2006, 5(2): 330−346. doi: 10.1128/EC.5.2.330-346.2006
[26]	Versele M, de Winde J H, Thevelein J M. A novel regulator of G protein signalling in yeast, Rgs2, downregulates glucose-activation of the cAMP pathway through direct inhibition of Gpa2[J]. The EMBO Journal, 1999, 18(20): 5577−5591. doi: 10.1093/emboj/18.20.5577
[27]	Fujita A, Lord M, Hiroko T, et al. Rax1, a protein required for the establishment of the bipolar budding pattern in yeast[J]. Gene, 2004, 327(2): 161−169. doi: 10.1016/j.gene.2003.11.021
[28]	Fisk H A, Yaffe M P. Mutational analysis of Mdm1p function in nuclear and mitochondrial inheritance[J]. The Journal of Cell Biology, 1997, 138(3): 485−494. doi: 10.1083/jcb.138.3.485
[29]	McConnell S J, Yaffe M P. Intermediate filament formation by a yeast protein essential for organelle inheritance[J]. Science, 1993, 260: 687−689. doi: 10.1126/science.8480179
[30]	Lee B N, Adams T H. Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development[J]. Molecular Microbiology, 1994, 14(2): 323−334. doi: 10.1111/j.1365-2958.1994.tb01293.x
[31]	Molnár Z, Mészáros E, Szilágyi Z, et al. Influence of fadA^G203R and ΔflbA mutations on morphology and physiology of submerged Aspergillus nidulans cultures[J]. Applied Biochemistry and Biotechnology, 2004, 118: 349−360. doi: 10.1385/ABAB:118:1-3:349
[32]	Shin K S, Park H S, Kim Y H, et al. Comparative proteomic analyses reveal that FlbA down-regulates gliT expression and SOD activity in Aspergillus fumigatus[J]. Journal of Proteomics, 2013, 87: 40−52. doi: 10.1016/j.jprot.2013.05.009
[33]	Han K H, Seo J A, Yu J H. Regulators of G-protein signalling in Aspergillus nidulans: RgsA downregulates stress response and stimulates asexual sporulation through attenuation of GanB (Galpha) signalling[J]. Molecular Microbiology, 2004, 53(2): 529−540. doi: 10.1111/j.1365-2958.2004.04163.x
[34]	Zhang H F, Tang W, Liu K Y, et al. Eight RGS and RGS-like proteins orchestrate growth, differentiation, and pathogenicity of Magnaporthe oryzae[J]. PLoS Pathogens, 2011, 7(12): e1002450. doi: 10.1371/journal.ppat.1002450
[35]	Li X, Zhong K L, Yin Z Y, et al. The seven transmembrane domain protein MoRgs7 functions in surface perception and undergoes coronin MoCrn1-dependent endocytosis in complex with Gα subunit MoMagA to promote cAMP signaling and appressorium formation in Magnaporthe oryzae[J]. PLoS Pathogens, 2019, 15(2): e1007382. doi: 10.1371/journal.ppat.1007382
[36]	韩长志. 禾谷炭疽菌RGS蛋白生物信息学分析[J]. 微生物学通报, 2014, 41(8):1582−1594. Han C Z. Bioinformatics analysis on regulators of G-protein signaling in Colletotrichum graminicola[J]. Microbiology China, 2014, 41(8): 1582−1594.
[37]	韩长志. 希金斯炭疽菌RGS蛋白生物信息学分析[J]. 生物技术, 2014, 24(1):36−41. Han C Z. Bioinformatics analysis on regulators of G-protein signaling in Coletotrichum higginsianum[J]. Biotechnology, 2014, 24(1): 36−41.
[38]	韩长志. 胶孢炭疽菌RGS蛋白生物信息学分析[J]. 河南师范大学学报(自然科学版), 2015(1):116−122. Han C Z. Bioinformatics analysis on regulators of G-protein signaling in Colletotrichum gloeosporioides[J]. Journal of Henan Normal University (Natural Science Edition), 2015(1): 116−122.
[39]	吴曼莉. 橡胶树胶孢炭疽菌G蛋白信号调控因子CgRGS1、CgRGS2和CgRGS7的克隆及生物学功能[D]. 海南: 海南大学, 2017. Wu M L. Cloning and biological function of G protein signaling factor CgRGS1, CgRGS2 and CgRGS7 in Colletotrichum gloeosporioides[D]. Hainan: Hainan University, 2017.

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