Growth adaptability analysis of <i>BpCCR</i>1 transgenic <i>Betula platyphylla</i> and selection of elite lines

Wang Chu; Wang Yang; Zou Jianjun; Peng Rusheng; Liu Guifeng; Jiang Jing

doi:10.12171/j.1000-1522.20200264

Journal of Beijing Forestry University > 2022 > 44(7): 52-62. > DOI: 10.12171/j.1000-1522.20200264

Wang Chu, Wang Yang, Zou Jianjun, Peng Rusheng, Liu Guifeng, Jiang Jing. Growth adaptability analysis of BpCCR1 transgenic Betula platyphylla and selection of elite lines[J]. Journal of Beijing Forestry University, 2022, 44(7): 52-62. DOI: 10.12171/j.1000-1522.20200264

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Growth adaptability analysis of BpCCR1 transgenic Betula platyphylla and selection of elite lines

1.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, Heilongjiang, China
2.
Jilin Provincial Academy of Forestry Sciences, Changchun 130022, Jilin, China
3.
Liaoning Provincial Institute of Poplar, Gaizhou 115200, Liaoning, China

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Received Date: August 28, 2020
Revised Date: January 19, 2021
Accepted Date: June 28, 2022
Available Online: June 30, 2022
Published Date: July 24, 2022

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Abstract

Abstract

Objective This study aims to conduct a joint analysis of the growth adaptability and stability of the 7-year-old BpCCR1 transgenic birch in 3 test locations, screen high-quality transgenic lines, provide guidance for environmental release and production experiments.
Method The tree height, diameter at breast height (DBH) and volume at Maoershan Experimental Forest Farm, Shidaohe Forest Farm and Experimental Forest Farm of northeastern China were measured with 21 BpCCR1 transgenic white birch and 1 wild-type white birch (WT) as the research objects. The AMMI model was used to carry out the interaction effects between the tested lines and the environment, genetic stability and growth adaptability.
Result The analysis of variance showed that the differences in height between lines, locations, and interactions between lines and locations were extremely significant (P < 0.01). The best lines for each test location were selected by taking the average volume plus the standard deviation of the tested plants at each test site as the selection criteria for elite transgenic lines. The elite lines selected from the Maoershan Experimental Forest Farm were FC18, FC29, FC40, whose volumes were 72.99%, 51.28%, 64.39% higher than the population average, respectively. The elite lines selected from the Shidaohe Forest Farm were FC7 and FC33, whose volumes were 73.98%, 81.51% higher than the population average, respectively. The elite lines selected from the Ecological Experiment Forest Farm were FC28 and FC29, whose volumes were 69.89% and 73.57% higher than the population average, respectively. The AMMI model was used to evaluate the stability of the height of the tested lines. The lines with better growth stability in the Maoershan Experimental Forest Farm and Ecological Experimental Forest Farm were WT, FC11, FC16, FC19, FC28, FC29 , FC30, FC31, C4. Lines with better growth stability in Shidaohe Forest Farm were FC2, FC7, FC13, FC14, FC18, FC27, FC33, FC40, FC41, C6, C10, C17, C19. 22 test lines were evaluated for stability according to IPC1 and the Dg value. The results showed that FC14, FC29, C19, FC27, FC41, WT, FC13, C4, C10, C6, FC16, FC7, FC28, FC40, C17, FC31, FC18 were high-yielding and stable-yielding lines; FC2, FC11, FC19, and FC33 were high-yielding and unstable-yielding lines; FC30 was a low- yielding and stable-yielding line.
Conclusion Both the sense and antisense BpCCR1 transgenes promot tree height of birch. 17 lines were selected based on their growth traits as high-yielding lines with good growth adaptation in the Maoershan Experimental Forest Farm, Shidaohe Forest Farm and Experimental Forest Farm.
- Betula platyphylla,
- BpCCR1,
- genetic transformation,
- afforestation experiment,
- growth adaptability

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Growth adaptability analysis of BpCCR1 transgenic Betula platyphylla and selection of elite lines

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