Types and quantitative analysis of anthocyanins in F1 hybrid among varieties in Rhododendron subgenus Tsutsusi

Xia Xi; Gong Rui; Feng Shucheng; Zhang Chunying

doi:10.12171/j.1000-1522.20210060

Journal of Beijing Forestry University > 2022 > 44(5): 105-114. > DOI: 10.12171/j.1000-1522.20210060

Xia Xi, Gong Rui, Feng Shucheng, Zhang Chunying. Types and quantitative analysis of anthocyanins in F₁ hybrid among varieties in Rhododendron subgenus Tsutsusi[J]. Journal of Beijing Forestry University, 2022, 44(5): 105-114. DOI: 10.12171/j.1000-1522.20210060

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Types and quantitative analysis of anthocyanins in F₁ hybrid among varieties in Rhododendron subgenus Tsutsusi

Shanghai Botanical Garden, Shanghai Urban Plant Resources Development and ApplicationEngineering Research Center, Shanghai 200231, China

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Received Date: February 18, 2021
Revised Date: March 19, 2021
Available Online: April 01, 2022
Published Date: May 24, 2022

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Abstract

Abstract

Objective Color is the major ornamental characteristics of plants, anthocyanins are the key compounds of flower colors. The types and contents of anthocyanins can provide insight into the mechanism of petal color.
Method In this study, 36 samples of three pairs of Rhododendron subgenus Tsutsusi parents and their hybrids were subjected to anthocyanins profiling in petals and their separation characteristics using UPLC –Q –TOF –MS.
Result This study identified 17 compounds, including peonindin 3-O-galactoside, delphinidin 3-galactoside, malvidin 3-O-galactoside, and petunidin 3-galactoside, which were detected for the first time in Rhododendron subgenus Tsutsusi. A majority of anthocyanins in Rhododendron subgenus Tsutsusi are cyanidin, peonidin, and petunidin in the form of arabinosides. Cyanidin 3-arabinoside, cyanidin 3-O-glucoside and peonidin are responsible for the red-colored petals in Rhododendron subgenus Tsutsusi. Delphinidin 3-arabinoside, delphinidin 3-O-glucoside, malvidin 3-arabinoside, and malvidin 3-O-glucoside contribute to the petal’s purple color, while petunidin 3-O-arabinoside contributes to their purplish red color. The anthocyanin segregation analysis revealed that peonidin and cyanidin had transgressive inheritance, whereas malvidin 3-arabinoside, malvidin 3-O-glucoside, and petunidin 3-O-arabinoside had paternal inheritance.
Conclusion In this study, we demonstrate the coloring mechanism of Rhododendron subgenus Tsutsusi, which provides a reference for parent selection in breeding.
- Rhododendron subgenus Tsutsusi,
- anthocyanin,
- floral color,
- HPLC-MS

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References

[1]	Tanaka Y, Ohmiya A. Seeing is believing: engineering anthocyanin and carotenoid biosynthetic pathways[J]. Current Opinion in Biotechnology, 2008, 19(2): 190−197. doi: 10.1016/j.copbio.2008.02.015
[2]	Du Q Z, Jerz G, Winterhalter P. Isolation of two anthocyanin sambubiosides from bilberry (Vaccinium myrtillus) by high-speed counter-current chromatography[J]. Journal of Chromatography A, 2004, 1045: 59−63. doi: 10.1016/j.chroma.2004.06.017
[3]	Zhang J J, Wang L S, Shu Q Y, et al. Comparison of anthocyanins in non-blotches and blotches of the petals of Xibei tree peony[J]. Scientia Horticulture, 2007, 114(2): 104−111. doi: 10.1016/j.scienta.2007.05.009
[4]	Li J B, Hashimoto F, Shimizu K, et al. A new acylated anthocyanin from the red flowers of Camellia hongkongensis and characterization of anthocyanins in the Section Camellia species[J]. Journal of Integrative Plant Biology, 2009, 51(6): 545−552. doi: 10.1111/j.1744-7909.2009.00828.x
[5]	Diaconeasa Z, Stirbu I, Xiao J B, et al. Anthocyanins, vibrant color pigments, and their role in skin cancer prevention[J/OL]. Biomedicines, 2020, 8(9): 336[2021−01−01]. https://doi.org/10.3390/biomedicines8090336.
[6]	Li J B, Hashimoto F, Shimizu K, et al. Anthocyanins from red flowers of Camellia cultivar ‘Dalicha’[J]. Phytochemistry, 2008, 69(18): 3166−3171.
[7]	de Loose R. The flower pigments of the Belgian hybrids of Rhododendron simsii and other species and varieties from Rhododendron subseries obtusum[J]. Phytochemistry, 1969, 8(1): 253−259. doi: 10.1016/S0031-9422(00)85822-7
[8]	de Loose R. Flavonoid glycosides in the petals of some Rhododendron species and hybrids[J]. Phytochemistry, 1970, 9(4): 875−879. doi: 10.1016/S0031-9422(00)85196-1
[9]	Mizuta D, Ban T, Miyajima I, et al. Comparison of flower color with anthocyanin composition patterns in evergreen azalea[J]. Scientia Horticulturae, 2009, 122(4): 594−602. doi: 10.1016/j.scienta.2009.06.027
[10]	Du H, Lai L M, Wang F, et al. Characterization of flower coloration in 30 Rhododendron species via anthocyanin and flavonol identification and quantitative traits[J]. Plant Biology, 2018, 20(1): 121−129. doi: 10.1111/plb.12649
[11]	李崇辉, 王亮生, 舒庆艳, 等. 迎红杜鹃花色素组成及花色在开花过程中的变化[J]. 园艺学报, 2008, 35(7): 1023−1030. doi: 10.3321/j.issn:0513-353X.2008.07.013 Li C H, Wang L S, Shu Q Y, et al. Pigments composition of petals and floral color change during the blooming period in Rhododendron mucronulatum[J]. Acta Horticulturae Sinica, 2008, 35(7): 1023−1030. doi: 10.3321/j.issn:0513-353X.2008.07.013
[12]	吴丽媛, 罗向东, 戴亮芳, 等. 杜鹃花色素的分离与鉴定分析[J]. 食品科学, 2011, 32(13): 19−23. Wu L Y, Luo X D, Dai L F, et al. Extraction and primary identification of anthocyanidins in Rhododendron flowers[J]. Food Science, 2011, 32(13): 19−23.
[13]	赵歌. 杜鹃(Rhododendron ripense)花色素的研究与应用[D]. 苏州: 苏州大学, 2012. Zhao G. Research and application of anthocyanidin extraction from flower of Rhododendron ripense [D]. Suzhou: Soochow University, 2012.
[14]	郑茜子, 赵冰, 曾慧敏, 等. 3 种不同颜色秦岭美容杜鹃花瓣中色素组成和含量比较分析[J]. 西北林学院学报, 2017, 32(1): 62−68. doi: 10.3969/j.issn.1001-7461.2017.01.10 Zheng X Z, Zhao B, Zeng H M, et al. Comparative analysis of composition and content of pigments in petals of three different colors of Rhododendron calophytum in Qinling Mountains[J]. Journal of Northwest Forestry University, 2017, 32(1): 62−68. doi: 10.3969/j.issn.1001-7461.2017.01.10
[15]	Chamberlain D F, Rae S J. A revision of Rhododendron IV subgenus Tsutsusi[J]. Edinburgh Journal of Botany, 1990, 47: 89−200.
[16]	Eeckhaut T, Keyser D, Huylenbroeck V, et al. Application of embryo rescue after interspecific crosses in the genus Rhododendron[J]. Plant Cell Tissue and Organ Culture, 2007, 89: 29−35. doi: 10.1007/s11240-007-9209-4
[17]	董雪娜, 陈希, 蒋甲福, 等. 非洲菊F代观赏性状的遗传表现[J]. 南京农业大学学报, 2015, 38(2): 226−232. doi: 10.7685/j.issn.1000-2030.2015.02.008 Dong X N, Chen X, Jiang J F, et al. Heredity of ornamental traits in F1 of Gerbera jamesonii Bolus[J]. Journal of Nanjing Agricultural University, 2015, 38(2): 226−232. doi: 10.7685/j.issn.1000-2030.2015.02.008
[18]	王欢, 孔滢, 郎利新, 等. 亚洲百合与大花卷丹杂种 F1 重要性状的遗传分析[J]. 华北农学报, 2017, 32(4): 114−121. doi: 10.7668/hbnxb.2017.04.019 Wang H, Kong Y, Lang L X, et al. Genetic analysis of important characters in F1 hybrids of Lilium Asiatic hybrids and L. leichtlinii var. maximowiczii[J]. Acta Agriculturae Boreali-Sinica, 2017, 32(4): 114−121. doi: 10.7668/hbnxb.2017.04.019
[19]	陈艳艳, 黄少华, 冷青云, 等. 不同红掌杂交组合 F1代重要形态性状的遗传分析[J]. 热带作物学报, 2019, 40(8): 1488−1494. doi: 10.3969/j.issn.1000-2561.2019.08.005 Chen Y Y, Huang S H, Leng Q Y, et al. Genetic analysis of key morphological characters in different cross combinations of anthurium cultivars[J]. Chinese Journal of Tropical Crops, 2019, 40(8): 1488−1494. doi: 10.3969/j.issn.1000-2561.2019.08.005
[20]	钟乃盛, 冯桂梅, 黄万坚, 等. 杜鹃红山茶种问杂交F1代实生苗主要性状的遗传表达[C]//中国园艺学会. 中国观赏园艺研究进展2012年会论文集. 北京: 中国林业出版社, 2012: 151−159. Zhong N S, Feng G M, Huang W J, et al. Genetic expressions of primary characteristics in the F1 seedlings obtained from camellia azalea ’s inter-specific hybridizations[C]//Chinese Society for Horticultural Science. Proceedings of the 2012 Annual Conference of the advances in Ornamental Horticulture of China. Beijing: China Forestry Publishing House, 2012: 151−159.
[21]	刘晓青, 苏家乐, 陈璐, 等. 杜鹃花品种(种)间杂交F1代主要观赏性状的遗传表现[J]. 江苏农业学报, 2012, 28(2): 459−460. doi: 10.3969/j.issn.1000-4440.2012.02.042 Liu X Q, Su J L, Chen L, et al. Genetic performance of ornamental traits in F1 inter varietal hybrids of Rhododendron[J]. Jiangsu Journal of Agricultural Sciences, 2012, 28(2): 459−460. doi: 10.3969/j.issn.1000-4440.2012.02.042
[22]	徐文姬. 野鸢尾与射干种间杂交后代遗传变异及花色形成机理研究[D]. 沈阳: 沈阳农业大学, 2018. Xu W J. Mechanism of flower color formation and genetic analysis of hybrid progenies derived from the cross of Iris dichotoma and I. domestica[D]. Shenyang: Shenyang Agricultural University, 2018.
[23]	陈越, 李纪元, 倪慧, 等. 杜鹃花花色苷遗传变异的研究[J]. 林业科学研究, 2013, 26(1): 81−87. doi: 10.3969/j.issn.1001-1498.2013.01.014 Chen Y, Li J Y, Ni H, et al. Research on genetic variations of anthocyanins in azalea petals[J]. Forest Research, 2013, 26(1): 81−87. doi: 10.3969/j.issn.1001-1498.2013.01.014
[24]	Wang L S, Hashimoto F, Shiraishi A, et al. Chemical taxonomy of the Xibei tree peony from China by floral pigmentation[J]. Journal of Plant Research, 2004, 117(1): 47−55.
[25]	de Riek J, de Keyser E, Calsyn E, et al. Ornamental crops[M]. Berlin: Springer, 2018: 237−271.
[26]	于晓南, 张启翔. 观赏植物的花色素苷与花色[J]. 林业科学, 2002, 38(3): 147−153. doi: 10.3321/j.issn:1001-7488.2002.03.026 Yu X N, Zhang Q X. Anthocyanin in ornamental plant and color express[J]. Scientia Silvae Sinicae, 2002, 38(3): 147−153. doi: 10.3321/j.issn:1001-7488.2002.03.026
[27]	张洁, 王亮生, 高锦明, 等. 贴梗海棠花青苷组成及其与花色的关系[J]. 园艺学报, 2011, 38(3): 527−534. Zhang J, Wang L S, Gao J M, et al. Identification of anthocyanins involving in petal coloration in Chaenomeles speciosa cultivars[J]. Acta Horticulturae Sinica, 2011, 38(3): 527−534.
[28]	李辛雷, 殷恒福, 范正琪, 等. 山茶芽变花色与花青苷的关系[J]. 中国农业科学, 2019, 52(11): 1961−1969. doi: 10.3864/j.issn.0578-1752.2019.11.010 Li X L, Yin H F, Fan Z Q, et al. The relationship between anthocyanins and flower colors of bud mutation in Camellia japonica[J]. Scientia Agricultura Sinica, 2019, 52(11): 1961−1969. doi: 10.3864/j.issn.0578-1752.2019.11.010
[29]	陶秀花, 袁媛, 徐怡倩, 等. 风信子花瓣花色苷组成分析[J]. 园艺学报, 2015, 42(2): 301−310. Tao X H, Yuan Y, Xu Y Q, et al. Anthocyanin profiles in petals of different Hyacinthus orientalis[J]. Acta Horticulturae Sinica, 2015, 42(2): 301−310.
[30]	Miyajima I, Ureshino K, Kobayashi N, et al. Flower color and pigments of intersubgeneric hybrid between white-flowered evergreen and yellow-flowered deciduous azaleas[J]. J Japan Soc Hort Sci, 2000, 69(3): 280−282. doi: 10.2503/jjshs.69.280
[31]	李霞, 吴钰滢, 封晔. 风信子不同花色品种花青素苷含量及相关基因表达分析[J]. 分子植物育种, 2020, 18(14): 4562−4571. Li X, Wu Y Y, Feng Y. Analysis of anthocyanin content and related gene expression in different varieties of Hyacinthus orientalis[J]. Molecular Plant Breeding, 2020, 18(14): 4562−4571.
[32]	Yamagishi M, Yoshida Y, Nakayama M. The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies (Lilium spp.) and regulates pigment quantity[J]. Molecular Breeding, 2012, 30(2): 913−925. doi: 10.1007/s11032-011-9675-6
[33]	Liu L, Zhang L Y, Wang S L, et al. Analysis of anthocyanins and flavonols in petals of 10 Rhododendron species from the Sygera Mountains in Southeast Tibet[J]. Plant Physiology and Biochemistry, 2016, 104(7): 250−256.
[34]	邓娇. 莲花瓣类黄酮色素分析及莲花瓣着色机理研究[D]. 武汉: 中国科学院武汉植物园, 2015. Deng J. The analysis of flavonoid pigments in lotus petals and the studies on the coloration mechanism of lotus petals[D]. Wuhan: Wuhan Botanical Garden, Chinese Academy of Sciences, 2015.

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Types and quantitative analysis of anthocyanins in F₁ hybrid among varieties in Rhododendron subgenus Tsutsusi