Types and quantitative analysis of anthocyanins in F1 hybrid among varieties in Rhododendron subgenus Tsutsusi

Xia Xi; Gong Rui; Feng Shucheng; Zhang Chunying

doi:10.12171/j.1000-1522.20210060

Journal of Beijing Forestry University > 2022 > 44(5): 105-114. > DOI: 10.12171/j.1000-1522.20210060

Xia Xi, Gong Rui, Feng Shucheng, Zhang Chunying. Types and quantitative analysis of anthocyanins in F₁ hybrid among varieties in Rhododendron subgenus Tsutsusi[J]. Journal of Beijing Forestry University, 2022, 44(5): 105-114. DOI: 10.12171/j.1000-1522.20210060

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Types and quantitative analysis of anthocyanins in F₁ hybrid among varieties in Rhododendron subgenus Tsutsusi

Shanghai Botanical Garden, Shanghai Urban Plant Resources Development and ApplicationEngineering Research Center, Shanghai 200231, China

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Received Date: February 18, 2021
Revised Date: March 19, 2021
Available Online: April 01, 2022
Published Date: May 24, 2022

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Abstract

Abstract

Objective Color is the major ornamental characteristics of plants, anthocyanins are the key compounds of flower colors. The types and contents of anthocyanins can provide insight into the mechanism of petal color.
Method In this study, 36 samples of three pairs of Rhododendron subgenus Tsutsusi parents and their hybrids were subjected to anthocyanins profiling in petals and their separation characteristics using UPLC –Q –TOF –MS.
Result This study identified 17 compounds, including peonindin 3-O-galactoside, delphinidin 3-galactoside, malvidin 3-O-galactoside, and petunidin 3-galactoside, which were detected for the first time in Rhododendron subgenus Tsutsusi. A majority of anthocyanins in Rhododendron subgenus Tsutsusi are cyanidin, peonidin, and petunidin in the form of arabinosides. Cyanidin 3-arabinoside, cyanidin 3-O-glucoside and peonidin are responsible for the red-colored petals in Rhododendron subgenus Tsutsusi. Delphinidin 3-arabinoside, delphinidin 3-O-glucoside, malvidin 3-arabinoside, and malvidin 3-O-glucoside contribute to the petal’s purple color, while petunidin 3-O-arabinoside contributes to their purplish red color. The anthocyanin segregation analysis revealed that peonidin and cyanidin had transgressive inheritance, whereas malvidin 3-arabinoside, malvidin 3-O-glucoside, and petunidin 3-O-arabinoside had paternal inheritance.
Conclusion In this study, we demonstrate the coloring mechanism of Rhododendron subgenus Tsutsusi, which provides a reference for parent selection in breeding.
- Rhododendron subgenus Tsutsusi,
- anthocyanin,
- floral color,
- HPLC-MS

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References (34)

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Types and quantitative analysis of anthocyanins in F₁ hybrid among varieties in Rhododendron subgenus Tsutsusi