Analysis of insect resistance ability of the serine protease inhibitor PtrSPI from <i>Populus tomentosa</i>

Yu Wenjing; Yang Shuai; Huang Ying; Diao Guiping

doi:10.12171/j.1000-1522.20210163

Journal of Beijing Forestry University > 2021 > 43(9): 51-58. > DOI: 10.12171/j.1000-1522.20210163

Yu Wenjing, Yang Shuai, Huang Ying, Diao Guiping. Analysis of insect resistance ability of the serine protease inhibitor PtrSPI from Populus tomentosa[J]. Journal of Beijing Forestry University, 2021, 43(9): 51-58. DOI: 10.12171/j.1000-1522.20210163

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Analysis of insect resistance ability of the serine protease inhibitor PtrSPI from Populus tomentosa

1.
School of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China
2.
Forestry Protection Institute, Heilongjiang Academy of Forestry, Harbin 150040, Heilongjiang, China

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Received Date: April 29, 2021
Revised Date: June 16, 2021
Available Online: July 13, 2021
Published Date: October 14, 2021

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Abstract

Abstract

Objective To lay the foundation of the development of new insect-resistant biological pesticides in trees, the insect-resistant function of the serine protease inhibitor PtrSPI of Populus tomentosa was studied.
Method To analyze the function of serine protease inhibitor gene PtrSPI, the promoter and the expression pattern under feeding stress of Lymantria dispar larvaes of the gene PtrSPI in P. tomentosa were studied. To discuss the anti-insect ability of PtrSPI, the food intake, the body mass, the mortality and the serine protease enzyme activities of L. dispar larvae were studied after using the eukaryotic recombinant protein PtrSPI feeding L. dispar larvaes.
Result The results showed that 5 elements related to plant resistance to disease and insects were in the promoter region of the serine protease inhibitor gene PtrSPI of P. tomentosa; the expression pattern of the gene PtrSPI in P. tomentosa leaves was firstly decreased and then increased after being fed by L. dispar larvae, and the peak was 2.03 times of the control at 30 h; under the high concentration of recombinant protein (300 and 500 mg/mL), the food intake and the body mass of L. dispar larvaes were significantly inhibited, the mortality of L. dispar larvaes was up to more than 60% after 4 and 6 days, respectively, and the activities of the serine protease in L. dispar larvaes were significantly increased at the beginning of feeding.
Conclusion This study verifies the insect resistance of the serine protease inhibitor PtrSPI of P. tomentosa, and it provides theoretical basis and research materials for further research and development of new non-pollution and insect-resistance biological pesticides.
- serine protease inhibitor,
- Populus tomentosa,
- insect-resistant function,
- biopesticide,
- Lymantria dispar

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Analysis of insect resistance ability of the serine protease inhibitor PtrSPI from Populus tomentosa