Regulation of flowering and protective enzyme gene expression in <i>Mikania micrantha</i> by exogenous hormone CPPU

Liao Qi; Liu Minghuan; Tian Siqi; Mao Xiangzhong; Zhao Ning

doi:10.12171/j.1000-1522.20210221

Journal of Beijing Forestry University > 2022 > 44(7): 90-96. > DOI: 10.12171/j.1000-1522.20210221

Liao Qi, Liu Minghuan, Tian Siqi, Mao Xiangzhong, Zhao Ning. Regulation of flowering and protective enzyme gene expression in Mikania micrantha by exogenous hormone CPPU[J]. Journal of Beijing Forestry University, 2022, 44(7): 90-96. DOI: 10.12171/j.1000-1522.20210221

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Regulation of flowering and protective enzyme gene expression in Mikania micrantha by exogenous hormone CPPU

1.
College of Life Science, Southwest Forestry University, Kunming 650224, Yunnan, China
2.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, Yunnan, China

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Received Date: June 08, 2021
Revised Date: June 30, 2021
Available Online: July 01, 2022
Published Date: July 24, 2022

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Abstract

Abstract

Objective Mikania micrantha is a class I alien invasive harmful plant, which relies on seeds to spread rapidly, causing significant ecological and economic losses. By exploring the mechanism of N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) on the reproductive regulation of M. micrantha, a new strategy was provided to curb the trend of M. micrantha’s rapid spread.
Method The number of inflorescences and florets of M. micrantha treated with CPPU was counted anatomically. The mechanism of inhibition of flowering by CPPU was analyzed by RNA-seq, and determined the enzyme activities of protective enzymes in flowering stage of M. micrantha.
Result (1) CPPU could effectively inhibit the flower formation of M. micrantha, with the increase of CPPU concentration, the number of inflorescences and florets decreased. After being treated with 5 mg/L CPPU, the number of inflorescences and florets decreased by 34.50% and 36.70%, respectively. There was no flower after 75 mg/L of CPPU treatment. (2) Transcriptome analysis revealed differential regulation of protective enzyme genes, it was found that, after being treated with 75 mg/l CPPU, a large number of DEGs related to pod and PAL were up-regulated; (3) the results of the protective enzyme activity indicated that CPPU treatment decreased the enzyme activity of PAL (phenylalanine ammonia-lyase), POD (peroxidase) and CAT (catalase), but increased the protective enzyme activity of PPO (polyphen oloxidase tyrosinase phenlase).
Conclusion 75 mg/L of CPPU can control the expression of protective enzyme gene of M. micrantha, which cause membrane lipid peroxidation damage and inhibit the flowering process, thus it can effectively control the reproduction and spread of M. micrantha.
- Mikania micrantha,
- CPPU,
- flowering,
- reproductive regulation

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References

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Regulation of flowering and protective enzyme gene expression in Mikania micrantha by exogenous hormone CPPU