Distribution and identification of phenolic constituents in the sapwood and heartwood of <i>Eucalyptus cloeziana</i>

Luo Jia; Ma Ruoke; Qiao Mengji; Fu Yunlin

doi:10.12171/j.1000-1522.20220372

Journal of Beijing Forestry University > 2023 > 45(6): 127-136. > DOI: 10.12171/j.1000-1522.20220372

Luo Jia, Ma Ruoke, Qiao Mengji, Fu Yunlin. Distribution and identification of phenolic constituents in the sapwood and heartwood of Eucalyptus cloeziana[J]. Journal of Beijing Forestry University, 2023, 45(6): 127-136. DOI: 10.12171/j.1000-1522.20220372

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Distribution and identification of phenolic constituents in the sapwood and heartwood of Eucalyptus cloeziana

College of Forestry, Guangxi University, Nanning 530000, Guangxi, China

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Received Date: September 12, 2022
Revised Date: March 16, 2023
Accepted Date: May 09, 2023
Available Online: May 11, 2023
Published Date: June 24, 2023

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Abstract

Abstract

Objective This paper aims to investigate the distribution of phenolic substances in the cell structure of sapwood and heartwood of Eucalyptus cloeziana and the main phenolic components of heartwood accumulation.
Method The distribution of phenolic substances in sapwood and heartwood was characterized by laser confocal Raman spectroscopy, and then the changes of phenolic components in xylem and their contents were identified by ultra performance liquid chromatography-mass spectrometry.
Result The Raman spectroscopy absorption intensity of heartwood was significantly higher than that of sapwood, and the phenolic content at the cell corner was significantly higher than that of the intercellular level and secondary wall. Further determination of the phenolic content of the sapwood and heartwood of E. cloeziana showed that the total polyphenol content in the heartwood was 22.378 mg/g higher than that of the sapwood, indicating that a large amount of phenolic substances was deposited at the cell corners during the formation of the heartwood. A total of 21 phenolic components were identified for the first time by UPLC-MS/MS from methanolic extracts of E. cloeziana wood, mainly containing phenolic acids and derivatives, flavonoids, ellagic acid derivatives, galloyglucose derivatives and hydrolysable tannins, with relatively high contents of ellagic acid, ellagic acid-rhamnoside, gallic acid, ellagic acid-pentoside and methylphloroglucinol-O-galloyl-glucose. The relative contents of ellagic acid and gallic acid were significantly higher in the heartwood than in the sapwood, and the quantitative analysis showed that ellagic acid was 0.560 and 9.283 mg/g in the sapwood and heartwood, respectively, and gallic acid was 0.019 and 0.043 mg/g in the sapwood and heartwood, respectively.
Conclusion It is clear that the phenolic composition is concentrated in the cell corners during the formation of the heartwood of E. cloeziana, and that ellagic acid is the main component accumulated in the heartwood.
- Eucalyptus cloeziana,
- heartwood,
- sapwood,
- phenolic,
- UPLC-MS/MS,
- extract

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References

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Distribution and identification of phenolic constituents in the sapwood and heartwood of Eucalyptus cloeziana

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