Advanced search
    Liu Jiaqi, Zhao Lijuan, Liu Lei, Huang Ruifen, Han Youzhi, Zhang Zhiwei, Cui Shaopeng, Gao Ruihe. Physiological adaptation mechanism of 4-age wintering larvae of Monochamus alternatus (Coleoptera: Cerambycidae) in response to low temperature stress[J]. Journal of Beijing Forestry University. DOI: 10.12171/j.1000-1522.20220438
    Citation: Liu Jiaqi, Zhao Lijuan, Liu Lei, Huang Ruifen, Han Youzhi, Zhang Zhiwei, Cui Shaopeng, Gao Ruihe. Physiological adaptation mechanism of 4-age wintering larvae of Monochamus alternatus (Coleoptera: Cerambycidae) in response to low temperature stress[J]. Journal of Beijing Forestry University. DOI: 10.12171/j.1000-1522.20220438

    Physiological adaptation mechanism of 4-age wintering larvae of Monochamus alternatus (Coleoptera: Cerambycidae) in response to low temperature stress

    • Objective In order to determine the physiological adaptation mechanism of the 4-age wintering larva of M. alternatus to low temperature stress.
      Method The 4-age wintering larva of M. alternatus were collected from Dexing, Jiangxi province, in mid-March of the 2021, seven temperature gradients of 25 ℃ (control), 4, 0, −5, −10, −15 and −20 ℃ and three time gradients of 30 min, 1 h and 3 h were set, the cold tolerance, the content of cold-resistant substances, the activity of antioxidant enzymes and the damage of midgut under low temperature stress were determined.
      Result (1) Near the supercooling point (−9.27 ± 0.90) ℃, the 50% lethal time was about 1.9 hours, and the 99% lethal time was about 2.8 days, Under low temperature stress below the supercooling point (−15 ℃), the lethal time of 50% and 99% is about 1/3 of that under low temperature treatment of −10 ℃. (2) After low temperature stress treatment, the water content of the 4-age wintering larvae did not change significantly, the fat content and glycogen content were significantly decreased after 3 h at 0 ℃ (P < 0.05), and the sorbitol content was significantly increased after 1 h at −5 ℃ (P < 0.05), and significantly decreased after 1 h at −20 ℃ (P < 0.05). (3) After low temperature stress treatment, the activities of antioxidant enzymes (SOD, POD, CAT) were significantly increased (P < 0.05). (4) The midgut of the 4-age wintering larvae of M. alternatus was decomposed after −15 ℃ treatment for 3 h, and vacuoles appeared between cells. After −20 ℃ treatment for 3 h, the midgut cells were ruptured, and the cell vacuoles escaped and filled the whole intestinal cavity, and the vacuoles disappeared.
      Conclusion This study clarified the physiological response of oxidative stress of the fourth instar overwintering larvae to low temperature stress, which could enrich and improve the study on cold tolerance mechanism of M. alternatus.
    • loading

    Catalog

      Turn off MathJax
      Article Contents

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return