- Scopus
- Chinese Science Citation Database (CSCD)
- A Guide to the Core Journal of China
- CSTPCD
- F5000 Frontrunner
- RCCSE
| Citation: |
Zhang Xiaomeng, Yin Kexin, An Keyue, Zhao Ziyan, Zhao Rui, Chen Shaoliang, Zhao Nan, Zhou Xiaoyang. Overexpression of Populus euphratica PeCPK7 enhancing salt tolerance in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2024, 46(11): 62-75. DOI: 10.12171/j.1000-1522.20230302
|
This study aimed to reveal the physiological and molecular regulation mechanisms of PeCPK7 in plant salt stress tolerance.
According to the CDS of PeCPK7 in NCBI genome database, the PeCPK7 was cloned, DNAMAN was used for amino acid sequence alignment, and Mega 7 software was used for evolutionary tree construction. The mechanism of PeCPK7 in salt stress was studied from the level of physiology, biochemistry and molecular biology by Arabidopsis thaliana overexpressed lines (PeCPK7-OE1, PeCPK7-OE2 and PeCPK7-OE3), the wild-type (WT) and vector control (VC) as experimental materials.
(1) PeCPK7 was highly similar to CPK7 in other species, and was closely related to PtrCPK7 family of Populus trichocarpa. (2) In salt-treated Populus euphratica seedlings, the relative expression of PeCPK7 reached the maximum after 6 h of salt stress, and recovered to the initial state at 48 h. (3) PeCPK7 was localized in the cytoplasm. (4) After salt treatment, the survival rate and root length of overexpression lines were significantly higher than those of WT and VC. (5) Na+ and H2O2 accumulated in the root were significantly lower than those of WT and VC, and Ca2+ was significantly higher than WT and VC. The Na+ efflux and K+ influx were significantly higher than WT and VC. (6) Under salt stress, the activity of antioxidant enzymes (SOD, POD, CAT) of overexpression lines were significantly higher than WT and VC. (7) The decrease of chlorophyll content, PSII. maximum optical quantum efficiency, actual photosynthetic quantum yield, relative electron transfer rate and the photosynthetic rates of overexpression lines were higher than those of WT and VC, while the intercellular CO2 concentration was lower than WT and VC.
Overexpression of PeCPK7 gene can improve salt stress tolerance of Arabidopsis thaliana, which will provide a theoretical basis for improving plant salt tolerance through genetic engineering.
| [1] |
Zhu J K. Abiotic stress signaling and responses in plants[J]. Cell, 2016, 167(2): 313−324. doi: 10.1016/j.cell.2016.08.029
|
| [2] |
Yang Y, Guo Y. Elucidating the molecular mechanisms mediating plant salt-stress responses[J]. New Phytologist, 2018, 217(2): 523−539. doi: 10.1111/nph.14920
|
| [3] |
Zhu J K. Plant salt tolerance[J]. Trends in Plant Science, 2001, 6(2): 66−71. doi: 10.1016/S1360-1385(00)01838-0
|
| [4] |
Valmonte G R, Arthur K, Higgins C M, et al. Calcium-dependent protein kinases in plants: evolution, expression and function[J]. Plant and Cell Physiology, 2014, 55(3): 551−569. doi: 10.1093/pcp/pct200
|
| [5] |
Luo Q, Feng J, Yang G, et al. Functional characterization of BdCIPK31 in plant response to potass ium deficiency stress[J]. Plant Physiology and Biochemistry, 2022, 192: 243−251. doi: 10.1016/j.plaphy.2022.10.014
|
| [6] |
Kim K N, Cheong Y H, Grant J J, et al. CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis[J]. Plant Cell, 2003, 15(2): 411−423. doi: 10.1105/tpc.006858
|
| [7] |
Zou J J, Li X D, Ratnasekera D, et al. Arabidopsis calcium-dependent protein kinases 8 and catalase3 function in abscisic acid-mediated signaling and H2O2 homeostasis in stomatal guard cells under drought stress[J]. Plant Cell, 2015, 27(5): 1445−1460. doi: 10.1105/tpc.15.00144
|
| [8] |
Grossi C E M, Santin F, Quintana S A, et al. Calcium-dependent protein kinase 2 plays a positive role in the salt stress response in potato[J]. Plant Cell Reports, 2022, 41(3): 535−548. doi: 10.1007/s00299-021-02676-7
|
| [9] |
Xu J, Tian Y S, Peng R H, et al. AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis[J]. Planta, 2010, 231(6): 1251−1260. doi: 10.1007/s00425-010-1122-0
|
| [10] |
Ma S Y, Wu W H. AtCPK23 functions in Arabidopsis responses to drought and salt stresses[J]. Plant Molecular Biology, 2007, 65(4): 511−518. doi: 10.1007/s11103-007-9187-2
|
| [11] |
Zhu S Y, Yu X C, Wang X J, et al. Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis[J]. Plant Cell, 2007, 19(10): 3019−3036. doi: 10.1105/tpc.107.050666
|
| [12] |
孙阳. 密胡杨抗逆生理生态初步研究[D]. 阿拉尔: 塔里木大学, 2021.
Sun Y. Preliminary study on stress resistance physiology and ecology of Euphratica [D]. Alar: Tarim University, 2021.
|
| [13] |
Chen S, Hawighorst P, Sun J, et al. Salt tolerance in populus: significance of stress signaling networks, mycorrhization, and soil amendments for cellular and whole-plant nutrition[J]. Environmental and Experimental Botany, 2014, 107: 113−124. doi: 10.1016/j.envexpbot.2014.06.001
|
| [14] |
Jia H, Liu G, Li J, et al. Genome resequencing reveals demographic history and genetic architecture of seed salinity tolerance in Populus euphratica[J]. Journal of Experimental Botany, 2020, 71(14): 4308−4320. doi: 10.1093/jxb/eraa172
|
| [15] |
Zhao R, Yin K, Chen S. Hydrogen sulphide signalling in plant response to abiotic stress[J]. Plant Biology (Stuttgart), 2022, 24(4): 523−531. doi: 10.1111/plb.13367
|
| [16] |
Zhang Y, Yin K, Yao J, et al. Populus euphratica GLABRA3 binds PLDdelta promoters to enhance salt tolerance[J]. International Journal of Molecular Sciences, 2023, 24(9): 8208.
|
| [17] |
Yao J, Shen Z, Zhang Y, et al. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance[J]. Journal of Experimental Botany, 2020, 71(4): 1527−1539. doi: 10.1093/jxb/erz493
|
| [18] |
张一南, 王洋, 张会龙, 等. 过表达胡杨PeRIN4基因拟南芥提高质膜H+-ATPase活性和耐盐性[J]. 北京林业大学学报, 2017, 39(11): 1−8.
Zhang Y N, Wang Y, Zhang H L, et al. Over-expression of PeRIN4 enhanced salinity tolerance through up-regulation of PM H+-ATPase in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2017, 39(11): 1−8.
|
| [19] |
Zhang H, Deng C, Wu X, et al. Populus euphratica remorin 6.5 activates plasma membrane H+-ATPases to mediate salt tolerance[J]. Tree Physiology, 2020, 40(6): 731−745. doi: 10.1093/treephys/tpaa022
|
| [20] |
Colin L, Ruhnow F, Zhu J K, et al. The cell biology of primary cell walls during salt stress[J]. Plant Cell, 2023, 35(1): 201−217. doi: 10.1093/plcell/koac292
|
| [21] |
Jiang S, Zhang D, Wang L, et al. A maize calcium-dependent protein kinase gene, ZmCPK4, positively regulated abscisic acid signaling and enhanced drought stress tolerance in transgenic Arabidopsis[J]. Plant Physiology and Biochemistry, 2013, 71: 112−120. doi: 10.1016/j.plaphy.2013.07.004
|
| [22] |
Ding C, Zhang W, Li D, et al. Effect of overexpression of JERFs on intracellular K+/Na+ balance in transgenic poplar (Populus alba × P. berolinensis) under salt stress[J]. Front Plant Science, 2020, 11: 1192. doi: 10.3389/fpls.2020.01192
|
| [23] |
Latz A, Mehlmer N, Zapf S, et al. Salt stress triggers phosphorylation of the Arabidopsis vacuolar K+ channel TPK1 by calcium-dependent protein kinases (CDPKs)[J]. Molecular Plant, 2013, 6(4): 1274−1289. doi: 10.1093/mp/sss158
|
| [24] |
Chen Y, Zhou X, Chang S, et al. Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice[J]. Biochemical and Biophysical Research Communications, 2017, 493(4): 1450−1456. doi: 10.1016/j.bbrc.2017.09.166
|
| [25] |
Zhang Y, Zhang Y, Yu J, et al. NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis[J]. BMC Genomics, 2019, 20(1): 990. doi: 10.1186/s12864-019-6325-6
|
| [26] |
侯梦娟, 朱新霞, 孔辉, 等. GhCDPK4基因的克隆和功能分析[J]. 西北农业学报, 2022, 31(2): 217−223.
Hou M J, Zhu X X, Kong H, et al. Cloning and functional analysis of GhCDPK4 gene [J]. Journal of Northwest Agricultural Sciences, 2019, 31(2): 217−223.
|
| [27] |
Wan Y, Zhang Y, Zhang M, et al. Shade effects on growth, photosynthesis and chlorophyll fluorescence parameters of three Paeonia species[J]. Peer Journal, 2020, 8: e9316. doi: 10.7717/peerj.9316
|
| [28] |
Makhtoum S, Sabouri H, Gholizadeh A, et al. Genomics and physiology of chlorophyll fluorescence parameters in Hordeum vulgare L. under drought and salt stresses[J]. Plants (Basel), 2023, 12(19): 3515.
|
| [29] |
Lu L, Wu X, Tang Y, et al. Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress[J]. Front Plant Science, 2022, 13: 1052463. doi: 10.3389/fpls.2022.1052463
|
| [30] |
潘欣. 转OsMAPK4、OsCDPK7基因耐盐碱水稻的筛选与抗性分析[D]. 哈尔滨: 东北农业大学, 2011.
Pan X. Screening and resistance analysis of saline-alkali resistant rice with OsMAPK4 and OsCDPK7 genes [D]. Harbin: Northeast Agricultural University, 2011.
|
| [31] |
申芳嫡. 转TaCPK7和OpIAP-p35基因小麦的分子检测及纹枯病抗性鉴定[D]. 咸阳: 西北农林科技大学, 2016.
Shen F D. Molecular detection and resistance identification of wheat with TaCPK7 and OPIA-P35 genes [D]. Xianyang: Northwest A&F University, 2016.
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: