Growth promotion and disease resistance function of Trichoderma ghanaiana ACCC30153

Objective To provide a theoretical basis and technical support for the field application of Trichoderma ghanense ACCC30153, the growth promotion and disease resistance function of it were analyzed.

Method The function of T. ghanense ACCC30153 on growth and disease resistance was preliminarily confirmed by the observation on poplar which irrigated with the conidium suspension of it. Then, the function of T. ghanense ACCC30153 was further determined by measuring the physiological and biochemical indexes and the expression differences of related functional genes.

Result (1) Irrigating different concentrations of T. ghanense ACCC30153 conidia suspension can promote the growth of poplar. The results showed that average plant height of poplar seedlings increased by 63.99% after 60 d of treatment with T. ghanense ACCC30153 conidia suspension. Moreover, physiological index measurement showed that the chlorophyll content in the leaves was significantly increased, and the net photosynthetic rate (P_n), transpiration rate (E), stomatal conductance (G_s) and intercellular CO₂ (C_i) were significantly higher than those in the control group after the administration of T. ghanense ACCC30153. (2) The disease resistance ability of poplar leaves irrigated with or without T. ghanense ACCC30153 conidia suspension were compared, the results showed that the disease resistance ability of poplar irrigated with T. ghanense ACCC30153 conidia suspension were higher than that without irrigating with T. ghanense ACCC30153 conidia suspension. The analysis of the activity of enzymes related to disease resistance (including PAL, SOD, CAT and POD) showed that T. ghanense ACCC30153 improved the ability of poplar disease resistance by increasing the activity of defense enzymes in the leaves. (3)The confrontation experiment showed that T. ghanense ACCC30153 had obvious antagonistic effect on various pathogenic fungi, and the real-time fluorescence quantitative RT-qPCR results showed that the lysozyme and proteolytic enzyme secreted by Trichoderma were involved in the antagonistic effect of T. ghanense ACCC30153 on pathogenic fungi.

Conclusion This study showed that T. ghanense ACCC30153 had obvious growth promotion and disease resistance to poplar.