Effects of drying and storage methods on contents of secondary metabolites in plant leaves

Liang Changming; Tian Di; Zhao Changti; Chen Bingrui; Mo Yien; Xia Qinglin; Huo Yifei

doi:10.12171/j.1000-1522.20240288

Journal of Beijing Forestry University > 2024 > 46(12): 82-91. > DOI: 10.12171/j.1000-1522.20240288

Liang Changming, Tian Di, Zhao Changti, Chen Bingrui, Mo Yien, Xia Qinglin, Huo Yifei. Effects of drying and storage methods on contents of secondary metabolites in plant leaves[J]. Journal of Beijing Forestry University, 2024, 46(12): 82-91. DOI: 10.12171/j.1000-1522.20240288

Citation:

PDF (1973 KB)

Effects of drying and storage methods on contents of secondary metabolites in plant leaves

State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China

More Information

Received Date: August 02, 2024
Revised Date: November 17, 2024
Accepted Date: December 06, 2024
Available Online: December 08, 2024

Graphical Abstract

Abstract

Abstract

Objective
This study aimed to provide guidance on pretreatment methods for plant leaves during field sampling, preservation, and experimentation by detecting changes in the contents of total phenol, flavonoid, and tannin in plant leaves after different pretreatments.
Method
The leaves of herbaceous plants, shrubs, broadleaved trees, and coniferous trees were selected as research objects. The drying pretreatment methods included vacuum freeze-drying, oven drying, microwave drying, natural sun drying, and natural shade drying. The storage pretreatment methods involved storage temperatures of −20 and −80 ℃ and storage durations of 15 and 30 d. The effects of different pretreatment methods on contents of total phenol, flavonoid, and tannin in plant leaves were compared.
Result
(1) After different drying treatments in the leaves of 8 plant species, the maximum content of total phenol in leaves mostly appeared in vacuum freeze-drying and microwave drying; the maximum flavonoid content of leaves mostly appeared in vacuum freeze-drying, oven drying and microwave drying; the maximum tannin content of leaves mostly appeared in vacuum freeze-drying and microwave drying. (2) The contents of total phenol, flavonoid and tannin in leaves of 8 species decreased with the increase of storage time, and the contents of secondary metabolites measured at −80 ℃ were mostly higher than those stored at −20 ℃.
Conclusion
In experiments detecting the content of foliar secondary metabolites, we recommend to complete the measurement as soon as possible after sample collection. Vacuum freeze-drying or microwave drying methods can be used for sample drying pretreatment. If long-distance transportation is required after sampling from field experiment sites, and storage conditions at −80 ℃ cannot be guaranteed, short-term transportation and storage at −20 ℃ is acceptable for measurement of the content of foliar secondary metabolites. Microwave drying, as a fast, safe, and efficient drying method, has strong applicability in the pretreatment of plant samples in laboratories.
- secondary metabolites,
- pretreatment methods,
- plant leaves,
- drying methods,
- storage methods

FullText(HTML)

References (38)

References

[1]	Salam U, Ullah S, Tang Z H, et al. Plant metabolomics: an overview of the role of primary and secondary metabolites against different environmental stress factors[J]. Life, 2023, 13(3): 706.
[2]	高丽君, 崔建华, 刘风云, 等. 植物次生代谢物的应用和开发[J]. 生物学通报, 2004, 39(7): 15−17. doi: 10.3969/j.issn.0006-3193.2004.07.007 Gao L J, Cui J H, Liu F Y, et al. Application and development of plant secondary metabolites[J]. Biological Bulletin, 2004, 39(7): 15−17. doi: 10.3969/j.issn.0006-3193.2004.07.007
[3]	董妍玲, 潘学武. 植物次生代谢产物简介[J]. 生物学通报, 2002(11): 17−19. doi: 10.3969/j.issn.0006-3193.2002.11.007 Dong Y L, Pan W X. A brief introduction of plant secondary metabolites[J]. Bulletin of Biology, 2002(11): 17−19. doi: 10.3969/j.issn.0006-3193.2002.11.007
[4]	华晓雨, 陶爽, 孙盛楠, 等. 植物次生代谢产物–酚类化合物的研究进展[J]. 生物技术通报, 2017, 33(12): 8. Hua X Y, Tao S, Sun S N, et al. Research progress on phenolic compounds of plant secondary metabolites[J]. Biotechnology Bulletin, 2017, 33(12): 8.
[5]	Samanta A, Das G, Das S K. Roles of flavonoids in plants[J]. Carbon, 2011, 100(6): 12−35.
[6]	Moyle J R, Burke J M, Fanatico A, et al. Palatability of tannin-rich sericea lespedeza fed to broilers[J]. Journal of Applied Poultry Research, 2012, 21(4): 891−896. doi: 10.3382/japr.2012-00559
[7]	王小菲, 高文强, 刘建锋, 等. 植物防御策略及其环境驱动机制[J]. 生态学杂志, 2015, 34(12): 3542−3552. Wang X F, Gao W Q, Liu J F, et al. Plant defensive strategies and environment-driven mechanisms[J]. Chinese Journal of Ecology, 2015, 34(12): 3542−3552.
[8]	宁夏化学分析测试协会. 枸杞中总酚含量的测定分光光度法: T/NAIA 097-2021[S]. 银川: 宁夏标准出版社, 2021. Ningxia Chemical Analysis and Testing Association. Determination of total phenolic content in Lycium barbarum L. spectrophotometric method: T/NAIA 097-2021[S]. Yinchuan: Ningxia Standards Press, 2021.
[9]	中华人民共和国农业部. 柑橘类水果及制品中总黄酮含量的测定: NY/T 2010-2011[S]. 北京: 中国标准出版社, 2011. The Ministry of Agriculture of the People's Republic of China. Determination of total flavonoids in citrus fruits and products: NY/T 2010-2011[S]. Beijing: Standards Press of China, 2011.
[10]	中华人民共和国国家质量监督检验检疫总局. 饲料中单宁的测定分光光度法: GB/T 27985-2011[S]. 北京: 中国标准出版社, 2011. General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China. Determination of tannins in feeds-spectrophotometric method: GB/T 27985-2011[S]. Beijing: Standards Press of China, 2011.
[11]	刘颖, 刘穗君, 李少华, 等. 烘干法水分测定仪测量物料含水率的预测及修正[J]. 包装工程, 2023, 44(增刊2): 82−86. Liu Y, Liu S J, Li S H, et al. Prediction and correction of material moisture content measured by drying moisture meter[J]. Packaging Engineering, 2023, 44(Suppl. 2): 82−86.
[12]	Parihar J S, Kumar S, Kumar L, et al. Development of novel cabinet solar dryer using UV sheet and its performance evaluation: an experimental study[J]. Solar Energy, 2022, 239: 1−9. doi: 10.1016/j.solener.2022.04.043
[13]	El-Sebaii A A, Shalaby S M. Solar drying of agricultural products: a review[J]. Renewable and Sustainable Energy Reviews, 2012, 16(1): 37−43. doi: 10.1016/j.rser.2011.07.134
[14]	Zahoor I, Mir T A, Ayoub W S, et al. Recent applications of microwave technology as novel drying of food:review[J]. Food and Humanity, 2023, 1: 92−103. doi: 10.1016/j.foohum.2023.05.001
[15]	胡静, 何劲, 杨丽梅, 等. 微波干燥法快速测定工业无水硫酸钠的水不溶物含量[J]. 盐科学与化工, 2023, 52(12): 37−40, 44. doi: 10.3969/j.issn.2096-3408.2023.12.010 Hu J, He J, Yang L M, et al. Rapid determination of water-insoluble matter in industrial anhydrous sodium sulfate by microwave drying method[J]. Journal of Salt Science and Chemical Industry, 2023, 52(12): 37−40, 44. doi: 10.3969/j.issn.2096-3408.2023.12.010
[16]	Colucci D, Maniaci R, Fissore D, et al. Monitoring of the freezing stage in a freeze-drying process using IR thermography[J]. International Journal of Pharmaceutics, 2019, 566: 488−499. doi: 10.1016/j.ijpharm.2019.06.005
[17]	Kulus D. Managing plant genetic resources using low and ultra-low temperature storage: a case study of tomato[J]. Biodiversity and Conservation, 2019, 28(5): 1003−1027.
[18]	陈青, 王玉珠, 梁延群, 等. 干燥方式对树莓总酚、总黄酮含量及其体外抗氧化活性的影响[J]. 北方园艺, 2019(13): 130−134. doi: 10.11937/bfyy.20184526 Chen Q, Wang Y Z, Liang Y Q, et al. Effect of different drying methods on total polyphenols, flavonoids content and antioxidant activity of Rubus corchorifolius L.[J]. Northern Horticulture, 2019(13): 130−134. doi: 10.11937/bfyy.20184526
[19]	Park C H, Yeo H J, Park C, et al. The effect of different drying methods on primary and secondary metabolites in Korean mint flower[J]. Agronomy, 2021, 11(4): 698. doi: 10.3390/agronomy11040698
[20]	吴兆喜, 王黔阳, 朱克松, 等. 不同干燥方式对百蕊草中两种黄酮醇苷含量影响[J]. 云南农业大学学报(自然科学), 2016, 31(4): 696−699. Wu Z X, Wang Q Y, Zhu K S, et al. Effect of different drying methods on the two flavonol glycosides content in Thesium chinense Turcz[J]. Journal of Yunnan Agricultural University (Natural Science), 2016, 31(4): 696−699.
[21]	Park M H, Seo J M, Kim S J, et al. Changes in the quality and secondary metabolites of kohlrabi during storage[J]. Korean Journal of Food Preservation, 2014, 21(5): 601−608. doi: 10.11002/kjfp.2014.21.5.601
[22]	Force L E, O’Hare T J, Wong L S, et al. Impact of cold storage on glucosinolate levels in seed-sprouts of broccoli, rocket, white radish and kohlrabi[J]. Postharvest Biology and Technology, 2007, 44(2): 175−178. doi: 10.1016/j.postharvbio.2006.11.014
[23]	石晓晨, 王蕾, 王尧尧, 等. 干燥方式对山楂总黄酮总有机酸含量的影响[J]. 山东科学, 2018, 31(5): 14−19. doi: 10.3976/j.issn.1002-4026.2018.05.003 Shi X Q, Wang L, Wang Y Y, et al. The effects of four different drying methods on total flavonoids and total organic acid contents in hawthorn[J]. Shandong Science, 2018, 31(5): 14−19. doi: 10.3976/j.issn.1002-4026.2018.05.003
[24]	Rahimmalek M, Goli S A H. Evaluation of six drying treatments with respect to essential oil yield, composition and color characteristics of Thymys daenensis subsp. daenensis. Celak leaves[J]. Industrial Crops and Products, 2013, 42: 613−619. doi: 10.1016/j.indcrop.2012.06.012
[25]	买买提依明, 徐立, 武春, 等. 新疆药桑黄酮和多酚的提取与分析研究初报[J]. 北方蚕业, 2008, 29(4): 12−14. doi: 10.3969/j.issn.1673-9922.2008.04.005 Mai M T Y M, Xu L, Wu C, et al. Xinjiang mulberry flavonoids and polyphenols extraction and analysis of the preliminary report[J]. North Sericulture, 2008, 29(4): 12−14. doi: 10.3969/j.issn.1673-9922.2008.04.005
[26]	李佳潞, 党辉, 杨梦洁, 等. 不同品种柿子的多酚单宁黄酮含量及其抗氧化活性研究[J]. 农产品加工, 2023(3): 57−61. Li J L, Dang H, Yang M J, et al. Contents of polyphenols tannins flavonoids and their antioxidant activities in different varieties of persimmon[J]. Farm Products Processing, 2023(3): 57−61.
[27]	王旭娟. 高粱单宁测定方法研究[D]. 郑州:河南工业大学, 2020. Wang X J. Study on the determination method of sorghum tannin[D]. Zhengzhou: Henan University of Technology, 2020.
[28]	贾敬鸾, 陈晓松, 陈正华. 云杉针叶的扫描电镜观察[J]. 云南大学学报(自然科学版), 1995, 32(增刊1): 25−27. Jia J L, Chen X S, Chen Z H. Observation of spruce needles by scanning electron microscopy[J]. Journal of Yunnan University (Natural Sciences Edition), 1995, 32(Suppl. 1): 25−27.
[29]	周晓剑, 杜官本. 刨花微波干燥特性研究[J]. 西南林业大学学报, 2007, 27(5): 73−77. doi: 10.11929/j.issn.2095-1914.2007.05.018 Zhou X J, Du G B. Study on microwave drying characteristics of wood flakes[J]. Journal of Southwest Forestry University, 2007, 27(5): 73−77. doi: 10.11929/j.issn.2095-1914.2007.05.018
[30]	喻芬, 万娜, 李远辉, 等. 中药材干燥过程中的理化性质变化规律与机制分析[J]. 中草药, 2021, 52(7): 2144−2153. Yu F, Wan N, Li Y H, et al. Analysis on change rule and mechanism in physical and chemical properties of Chinese herbal medicines during drying[J]. Chinese Traditional and Herbal Drugs, 2021, 52(7): 2144−2153.
[31]	Mathieu Y, Vidal J D, Arribas M L, et al. Molecular oxygen lignin depolymerization: an insight into the stability of phenolic monomers[J]. ChemSusChem, 2020, 13(17): 4743−4758. doi: 10.1002/cssc.202001295
[32]	刘曜儒, 邓小银, 宁华清, 等. 不同干燥技术对罗汉果干品质的影响[J]. 轻工科技, 2017, 33(5): 18−19. Liu Y R, Deng X Y, Ning H Q, et al. Effect of different drying techniques on dry quality of monahan fruit[J]. Light Industry Science and Technology, 2017, 33(5): 18−19.
[33]	Vinu R, Polisetti S, Madras G. Dye sensitized visible light degradation of phenolic compounds[J]. Chemical Engineering Journal, 2010, 165(3): 784−797. doi: 10.1016/j.cej.2010.10.018
[34]	Liu D, Li P, Han Y, et al. Low temperature storage of southern rice black-streaked dwarf virus-infected rice plants cannot sustain virus transmission by the vector[J]. Journal of Economic Entomology, 2016, 109(1): 57. doi: 10.1093/jee/tov277
[35]	Mulinacci N, Innocenti M, Bellumori M, et al. Storage method, drying processes and extraction procedures strongly affect the phenolic fraction of rosemary leaves: an HPLC/DAD/MS study[J]. Talanta, 2011, 85(1): 167−176. doi: 10.1016/j.talanta.2011.03.050
[36]	王顺民, 谭玉霞, 韩永斌, 等. 热风与微波及其联合干燥对菠菜干制效果的影响[J]. 食品科学, 2012, 33(20): 80−84. Wang S M, Tan Y X, Han Y B, et al. Effect of hot air, microwave and combined drying on the drying effect of spinach[J]. Food Science, 2012, 33(20): 80−84.
[37]	Agustian P, Ruriani E. Some properties of aloe vera gel powder prepared by microwave and oven drying[C]//Proceedings of IOP Conference Series: Earth and Environmental Science. Yogyakarta: IOP Publishing, 2024: 012052.
[38]	杜传来, 叶洪梅, 康永真. 香椿芽微波干燥特性的研究[J]. 保鲜与加工, 2007(1): 35−38. doi: 10.3969/j.issn.1009-6221.2007.01.013 Du C L, Ye H M, Kang Y Z. Study on microwave drying characteristics of Chinese toon[J]. Preservation and Processing, 2007(1): 35−38. doi: 10.3969/j.issn.1009-6221.2007.01.013

Cited By

Cited by

Periodical cited type(2)

1.	黎浩然，曾志斌，季璇，李泓昊. 一个珍贵的岭南明代木结构建筑病害诊断及修缮案例剖析. 广州建筑. 2025(01): 91-96 .
2.	周海宾，韩旭，黄磊，王卫滨，王双永. 古建筑木构件开裂机制、评估与加固研究进展. 木材科学与技术. 2024(01): 13-22 .

Other cited types(1)

Get Citation

PDF

XML

Article views (189) PDF downloads (43) Cited by(3)

Turn off MathJax

Article Contents

Abstract

References

Effects of drying and storage methods on contents of secondary metabolites in plant leaves

Abstract

References

Cited by

Periodical cited type(2)

Other cited types(1)

Catalog

Export File

Citation

Format

Content