Objective This paper aims to verify the synergistic effects of combined application of Paxillus involutus and biochar on Populus davidiana × P. alba seedlings by exploring their growth-promoting functions, so as to provide data support for subsequent research, and offer a theoretical basis for high-quality seedling cultivation and ecological environment improvement in northeastern China.
Method Using Populus davidiana × P. alba seedlings as the research objects, four treatments were established: blank control, sole application of Paxillus involutus, sole application of biochar, and combined application of Paxillus involutus and biochar. Plant physiological and biochemical experimental methods were employed to measure growth and physiological indices, including seedling height, ground diameter, leaf area, biomass, photosynthetic pigments, cellulose, soluble protein, and soluble sugar content, as well as root growth under each treatment.
Result Growth indices: seedling height, ground diameter, and leaf area significantly increased. Compared with blank control, the combined application treatment improved these indices by 23.2%, 16.7%, and 17.06%, respectively. The sole application of Paxillus involutus increased them by 17.5%, 29.5%, and 16.23%, respectively. Physiological indics: chlorophyll a, cellulose, soluble sugar, and soluble protein content in leaves significantly rose. The combined application treatment enhanced these above by 10.03%, 6.23%, 19.63%, and 85.51%, respectively, while chlorophyll b decreased by 1.77%. The sole Paxillus involutus treatment increased them by 6.72%, 5.84%, 9.37%, and 45.6%, respectively, with chlorophyll b declining by 6.32%. Additionally, the combined application markedly improved root growth in Populus davidiana × P. alba. The roots of Populus davidiana × P. alba seedlings treated with both biochar and Paxillus involutus showed a 145.51% increase in root length and an 86.82% increase in root surface area compared with control.
Conclusion The combined application of Paxillus involutus and biochar can significantly promote the growth and physiological activity of Populus davidiana × P. alba seedlings, demonstrating a notable synergistic effect. The findings of this study provide both theoretical and practical foundations for improving seedling quality, enhancing afforestation survival rates, and promoting the sustainable development of ecological forestry.
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